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Recombinant Mouse Slit1 Protein, CF

Catalog #: 5199-SL
5 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
5199-SL-050
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Citations (5)
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse Slit1 Protein, CF Summary

Product Specifications

Purity
>80%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to enhance neurite outgrowth of dissociated E13 chick embryonic dorsal root ganglia (DRG) neurons. Able to significantly enhance neurite outgrowth when immobilized as a 3 μL droplet containing 90 ng on a nitrocellulose-coated microplate.
Source
Chinese Hamster Ovary cell line, CHO-derived mouse Slit1 protein
Cys34-Glu900, with a C-terminal 10-His tag
Accession #
Q80TR4
N-terminal Sequence
Analysis
Cys34
Predicted Molecular Mass
97.6 kDa
SDS-PAGE
110 kDa, under reducing conditions

Product Datasheets

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5199-SL

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

5199-SL

Formulation Lyophilized from a 0.2 μm filtered solution in MOPS and NaCl.
Reconstitution Reconstitute at 200 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Slit1

Slit1 is a member of the Slit family of large secreted axon guidance molecules that are ligands for Robo receptors (1, 2). Like other mammalian family members, the 1531 amino acid (aa), ~200 kDa Slit1 contains a 33 aa signal sequence followed by 23 leucine-rich repeats (LRR, aa 34 - 900) and 9 EGF-like sequences (aa 930 - 1451) (2). Mammalian Slits also contain a laminin-G domain between EGF6 and EGF7 (aa 1163 - 1336), and a C-terminal cysteine-rich domain (cysteine knot; aa 1456 - 1531) (2). Heparin sulfates are required for interaction of Robo with Slit LRR domains (2, 3). Mouse Slit1 shares 99, 96, 89, 87 and 80% aa identity with rat, human, canine, Xenopus and zebrafish Slit1, respectively, within the LRR domains. Mouse Slits 1, 2 and 3 share 68 - 74% aa identity within the LRR domains. Slit1 and Slit2 (or in some cases Slit3) are expressed in complementary locations during development of the optic and olfactory tracts and the forebrain, and appear to work together to mediate Robo guidance of retinal, olfactory, hippocampal and motor axons (1, 4 - 9). Deletion of either Slit1 or Slit2 has less effect than deletion of both, which allows axons to wander from tracts and inappropriately cross or recross the midline (4, 5, 7 - 9). In the injured spinal cord, presence of Slit1 along with Slit3 and Netrin-1 may be responsible for failure of axons to regenerate in the adult CNS (10). Slit1 also promotes dendrite growth and branching of cortical neurons indicating it may exert important influence on the final morphology of cortical neurons (11). Although Slit1 has mainly been found in the fetal and adult brain, it is also detected in the heart and kidney. The C-terminal cysteine knot, which may mediate interaction with other proteins, is absent in the rat brain splicing variant, Slit1 alpha (12).

References
  1. Yuan, W. et al. (1999) Dev. Biol. 212:290.
  2. Hohenester, E. (2008) Biochem. Soc. Trans. 36:251.
  3. Hussain, S-A. et al. (2006) J. Biol. Chem. 281:39693.
  4. Thompson, H. et al. (2006) Dev. Biol. 296:476.
  5. Plump, A. S. et al. (2002) Neuron 33:219.
  6. Cho, J. H. et al. (2007) J. Neurosci. 27:9094.
  7. Nguyen-Ba-Charvet, K. T. et al. (2002) J. Neurosci. 22:5473.
  8. Bagri, A. et al. (2002) Neuron 33:233.
  9. DiMeglio, T. et al. (2008) J. Neurosci. 28:6285.
  10. Wehrle, R. et al. (2005) Eur. J. Neurosci. 22:2134.
  11. Whitford, K. L. et al. (2002) Neuron 33:47.
  12. Tanno, T. et al. (2004) J. Biochem. 136:575.
Long Name
SLIT Homolog 1
Entrez Gene IDs
6585 (Human); 20562 (Mouse)
Alternate Names
KIAA0813; MEGF4; MEGF4slit (Drosophila) homolog 1; MGC164811; Multiple EGF-like domains protein 4; Multiple epidermal growth factor-like domains protein 4; SLIL1; slit homolog 1 (Drosophila); slit homolog 1 protein; Slit1; Slit-1slit1; SLIT3

Citations for Recombinant Mouse Slit1 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. SponGee: A Genetic Tool for Subcellular and Cell-Specific cGMP Manipulation
    Authors: O Ros, Y Zagar, S Ribes, S Baudet, K Loulier, S Couvet, D Ladarre, A Aghaie, A Louail, C Petit, Y Mechulam, Z Lenkei, X Nicol
    Cell Rep, 2019-06-25;27(13):4003-4012.e6.
    Species: Mouse
    Sample Types: Transfected Whole Cells
    Applications: Bioassay
  2. Robo signalling controls pancreatic progenitor identity by regulating Tead transcription factors
    Authors: S Escot, D Willnow, H Naumann, S Di Frances, FM Spagnoli
    Nat Commun, 2018-11-30;9(1):5082.
    Species: Human
    Sample Types: Transfected Whole Cells
    Applications: Bioassay
  3. Robo1 modulates proliferation and neurogenesis in the developing neocortex.
    Authors: Yeh M, Gonda Y, Mommersteeg M, Barber M, Ypsilanti A, Hanashima C, Parnavelas J, Andrews W
    J Neurosci, 2014-04-16;34(16):5717-31.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  4. Spontaneous activity regulates Robo1 transcription to mediate a switch in thalamocortical axon growth.
    Authors: Mire E, Mezzera C, Leyva-Diaz E, Paternain AV, Squarzoni P, Bluy L, Castillo-Paterna M, Lopez MJ, Peregrin S, Tessier-Lavigne M, Garel S, Galceran J, Lerma J, Lopez-Bendito G
    Nat. Neurosci., 2012-07-08;15(8):1134-43.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  5. The chemorepellent Slit3 promotes monocyte migration.
    Authors: Geutskens SB, Hordijk PL, van Hennik PB
    J. Immunol., 2010-11-15;185(12):7691-8.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay

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