Recombinant Mouse SLITRK2 Protein, CF

Catalog #: 9965-SK Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
9965-SK-050

Recombinant Mouse SLITRK2 Protein SDS-PAGE

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse SLITRK2 Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability to inhibit neurite outgrowth of dissociated E13 chick embryonic dorsal root ganglia (DRG) neurons. Able to significantly inhibit neurite outgrowth when immobilized as a 3 µL droplet containing 200-400 ng on a nitrocellulose-coated microplate.

Source

Mouse myeloma cell line, NS0-derived mouse SLITRK2 protein
Arg22-Leu622, with a C-terminal 6-His tag

Accession #

Q810C0

N-terminal Sequence
Analysis

Arg22

Predicted Molecular Mass

69 kDa

SDS-PAGE

86-98 kDa, reducing conditions

Product Datasheets

9965-SK

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9965-SK

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 250 μg/mL in water.
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	12 months from date of receipt, ≤ -20 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Scientific Data

SDS-PAGE

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2 μg/lane of Recombinant Mouse SLITRK2 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® blue staining, showing bands at 86-98 kDa respectively.

Reconstitution Calculator

Background: SLITRK2

SLITRK2 (Slit and Trk-like family member 2) is an approximately 110 kDa type I transmembrane member of the SLITRK family of proteins which contain a slit-like extracellular region and a Trk-like cytoplasmic region (1). The extracellular domain (ECD) of mature mouse SLITRK2 contains 6 leucine rich repeats (LRR) followed by a C-terminal LRR domain, followed by 6 more LRR flanked by a pair of N- and C-terminal LRR domains (2, 3). Within the ECD, mouse SLITRK2 shares 98% and 99% amino acid sequence identity with human and rat SLITRK2, respectively. SLITRK2 is expressed in multiple regions of the brain, particularly the cerebral cortex and hippocampus (4). It can suppress neurite outgrowth and promote the formation of excitatory and inhibitory presynaptic structures (3-5). The synaptogenic function is dependent on the interaction of SLITRK2 with select isoforms of PTP-sigma (5, 6). In humans, mutations of SLITRK2 are associated with bipolar disorder and schizophrenia (7, 8).

References