Recombinant Mouse ST7/LRP12 Protein, CF

Catalog #: 7910-S7 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
7910-S7-050

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse ST7/LRP12 Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. When Recombinant Mouse ST7/LRP12 is immobilized at 2 μg/mL (100 μL/well), the concentration of Recombinant Human LRPAP (Catalog # 4296-LR) that produces 50% of the optimal binding response is approximately 0.5-2.5 μg/mL.

Source

Chinese Hamster Ovary cell line, CHO-derived mouse ST7/LRP12 protein
Glu33-Thr491, with a C-terminal 6-His tag

Accession #

Q8BUJ9

N-terminal Sequence
Analysis

Glu33

Predicted Molecular Mass

52.4 kDa

SDS-PAGE

65-80 kDa, reducing conditions

Product Datasheets

7910-S7

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

7910-S7

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 200 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: ST7/LRP12

ST7 (Suppressor of Tumorigenicity 7), also known as RAY1, TSG7 and FAM4A1, is a type I transmembrane protein belonging to the LDLR (low density lipoprotein receptor) superfamily and is designated LRP12 (1‑3). The mouse ST7 cDNA encodes 858 amino acids (aa) including a 32 aa signal sequence, a 460 aa extracellular domain (ECD) containing two CUB domains and five LDLR class A domains, a 21 aa transmembrane domain, and a 345 aa cytoplasmic domain containing motifs implicated in endocytosis and signal transduction (1, 2). Mouse ST7 shares 97% aa sequence homology rat, and 95% with human, bovine, equine and porcine ST7 within the ECD. A mouse ST7 splicing variant lacks aa 27‑45, and another potential variant has an alternate start site at aa 188. ST7 is widely expressed in normal tissues, especially on fibroblasts (1, 4). Highest mRNA levels are detected in heart and skeletal muscle (1). ST7 was originally proposed to be a tumor suppressor protein, but it is not consistently down‑regulated in a variety of cancers, either by mutation or loss of heterozygosity (1, 4‑7). In certain cancers, expression may even be up‑regulated (8). Expression may be associated with down‑regulated expression of extracellular matrix molecules that are involved in remodeling, such as SPARC, IGFBP5 and several matrix metalloproteinases, and modulation of in vivo tumorigenicity (4, 5).

References

Qing, J. et al. (1999) Oncogene 18:335.
Battle, M.A. et al. (2003) Biochemistry 42:7270.
Vincent, J.B. et al. (2002) Genomics 80:283.
Zenclusen, J.C. et al. (2001) Nat. Genet. 27:392.
Hooi, C.F. et al. (2006) Oncogene 25:3924.
Sivasundaram, K. et al. (2003) Oncol. Rep. 10:1737.
Dong, S.M. and D. Sidransky (2002) Clin. Cancer Res. 8:2939.
Garnis, C. et al. (2004) Oncogene 23:2582.

Long Name

Low Density Lipoprotein Receptor-related 12

Entrez Gene IDs

29967 (Human); 239393 (Mouse)

Alternate Names

DKFZp762O2113; FAM4A1; LRP12; Protein FAM4A1; Protein HELG; RAY1; SEN4; ST7; subfamily A, member 1; suppression of tumorigenicity 7 (breast); suppression of tumorigenicity 7; suppressor of tumorigenicity 7 protein; TSG7