Recombinant Mouse VISTA/B7-H5/PD-1H Fc Chimera Protein, CF

Catalog #: 7005-B7 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
7005-B7-050

Recombinant Mouse VISTA/B7-H5/PD-1H Fc Chimera Protein Bioactivity

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse VISTA/B7-H5/PD-1H Fc Chimera Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE with silver staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability to inhibit anti-CD3 antibody induced IL-2 secretion in human T lymphocytes. The ED₅₀ for this effect is 0.5-3 μg/mL.

Source

Mouse myeloma cell line, NS0-derived mouse VISTA/B7-H5/PD-1H protein

Mouse VISTA/B7-H5/PD-1H (Phe33-Ala191) Accession # NP_001153044	IEGRMDP	Mouse IgG_2A (Glu98-Lys330)
N-terminus		C-terminus

Accession #

Q9D659

N-terminal Sequence
Analysis

Phe33

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

45 kDa (monomer)

SDS-PAGE

60-70 kDa, reducing conditions

Product Datasheets

7005-B7

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

7005-B7

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity

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Recombinant Mouse VISTA/B7-H5/PD-1H Fc Chimera (Catalog # 7005-B7) inhibits anti-CD3 antibody-induced IL-2 secretion in human T lymphocytes. The ED₅₀ for this effect is 0.5-3 μg/mL.

Reconstitution Calculator

Background: VISTA/B7-H5/PD-1H

Platelet receptor Gi24, also known as Dies1, VISTA, SISP1 and B7-H5, is a 55-65 kDa transmembrane glycoprotein with homology to B7-like immune co-stimulatory molecules (1, 2). It is distinct from HHLA2 which is also known as B7-H5 (3, 4). Mature mouse Gi24 contains a 159 amino acid (aa) extracellular domain (ECD) with one V-type Ig-like domain, a 21 aa transmembrane segment, and a 97 aa cytoplasmic domain. Within the ECD, mouse Gi24 shares 70% and 78% aa sequence identity with human and rat Gi24, respectively (5). The 30 kDa ECD can be shed by MT1-MMP, with a 25-30 kDa fragment remaining in the membrane (6). Gi24 promotes both MT1-MMP expression and the MT1-MMP mediated activation of MMP-2 (6). Gi24 supports the differentiation of embryonic stem cells (ESC) and enhances BMP-4 induced signaling in ESC, but it is also down-regulated following BMP-4 exposure (7, 8). It binds to BMP-4 directly and also associates with the type I BMP receptor Activin RIB/ALK-4 (7, 8). Gi24 is expressed on the surface of naïve CD4⁺ T cells and regulatory T cells (5). It is up-regulated in vivo on activated monocytes and dendritic cells (8). Gi24 inhibits CD4⁺ and CD8⁺ T cell proliferation and their production of IL-2 and IFN-gamma (5). Its expression on tumor cells attenuates the anti-tumor immune response and enables more rapid tumor progression (5). In contrast, Gi24 limits disease progression in the autoimmune disease model EAE (5). Gi24 is additionally expressed on mature adipocytes, particularly in fasting mice (9).

References

Jung, K. and I. Choi (2013) Immune Netw. 13:184.
Wilcox, R.A. et al. (2012) Eur. J. Haematol. 88:465.
Zhu, Y. et al. (2013) Nat. Commun. 4:2043.
Zhao, R. et al. (2013) Proc. Natl. Acad. Sci. USA 110:9879.
Wang, L. et al. (2011) J. Exp. Med. 208:577.
Sakr, M.A. et al. (2010) Cancer Sci. 101:2368.
Aloia, L. et al. (2010) J. Biol. Chem. 285:7776.
Parisi, S. et al. (2012) FASEB J. 26:3957.
Ren, G. et al. (2013) PLoS ONE 8:e65531.

Entrez Gene IDs

64115 (Human); 74048 (Mouse); 690899 (Rat); 102116468 (Cynomolgus Monkey)

Alternate Names

4632428N05Rik; B7H5; B7-H5; C10orf54; chromosome 10 open reading frame 54; Dies1; Gi24; PD1H; PD-1H; platelet receptor Gi24; PP2135; SISP1; stress induced secreted protein 1; VISTA; VSIR