Recombinant Mouse VSIG3 Fc Chimera Protein, CF

Catalog #: 9367-VS Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
9367-VS-050

Recombinant Mouse VSIG3 Fc Chimera Protein Bioactivity

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse VSIG3 Fc Chimera Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability to inhibit anti-CD3 antibody induced IL-17 secretion by human peripheral blood mononuclear cells (PBMC). The ED₅₀ for this effect is 1-5 μg/mL

Source

Mouse myeloma cell line, NS0-derived mouse VSIG3 protein

Mouse VSIG3 (Leu23-Gly245) Accession # P0C673	IEGRMDP	Mouse IgG_2a (Glu98-Lys330)
N-terminus		C-terminus

Accession #

P0C673

N-terminal Sequence
Analysis

Leu23

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

51 kDa

SDS-PAGE

60-68 kDa, reducing conditions

Product Datasheets

9367-VS

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9367-VS

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 200 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity

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Recombinant Mouse VSIG3 Fc Chimera (Catalog # 9367-VS) inhibits anti-CD3 antibody induced IL-17 secretion in human PBMC. The ED₅₀ for this effect is 1-5 μg/mL.

Reconstitution Calculator

Background: VSIG3

VSIG3 (V-set and immunoglobulin domain containing 3) also known as IgSF11 (Immunoglobulin superfamily member 11), is an approximately 50 kDa type I transmembrane adhesion protein of the Ig superfamily. Mature mouse VSIG3 consists of a 218 amino acid (aa) extracellular domain (ECD) containing one V-type Ig-like domain and one Ig-like C2-type domain, a 21 aa transmembrane domain, and a 167 aa cytoplasmic domain. Within the ECD, mouse VSIG3 shares 95% and 98% aa identity with human and rat VSIG3, respectively. VSIG3 is expressed on epithelial and endothelial cells, neurons and glial cells, and platelets (1). It localizes to epithelial tight junctions and mediates homophilic in trans cell adhesion (1-3). VSIG3 also localizes to neuronal postsynaptic densities where it recruits the GluA1 and GluA2 subunits of AMPA receptors and supports excitatory synaptic transmission (4). In zebrafish, VSIG3 is expressed in melanophores and their precursors and plays a role in the development and patterning of pigment cells (5). VSIG-3 expression is elevated in colorectal cancers and hepatocellular carcinomas as well as
intestinal-type gastric cancers. Suppression of VSIG-3 by siRNA retarded the growth of gastric cancer cells, suggesting that VSIG-3 is a good candidate for gastric cancer immunotherapy (6).

References

Raschperger, E. et al. (2004) J. Biol. Chem. 279:796.
Katoh, M. and M. Katoh (2003) Int. J. Oncol. 23:525.
Harada, H. et al. (2005) J. Cell. Physiol. 204:919.
Jang, S. et al. (2016) Nat. Neurosci. 19:84.
Eom, D.S. et al. (2012) PLoS Genet. 8:e1002899.
Watanabe T, et al. (2005) Cancer Sci. 96:498.

Long Name

V-Set and Immunoglobulin Domain Containing 3

Entrez Gene IDs

152404 (Human); 207683 (Mouse); 303926 (Rat); 102146257 (Cynomolgus Monkey)

Alternate Names

Brain and testis-specific immunoglobulin superfamily protein; BTIGSF; BT-IgSF; cancer/testis antigen 119; CT119; CXADR like 1; CXADRL1; IGSF11; Igsf13; immunoglobulin superfamily member 11; immunoglobulin superfamily, member 11; MGC35227; V-set and immunoglobulin domain containing 3; V-set and immunoglobulin domain-containing protein 3; VSIG3; VSIG3brain and testis-specific immunoglobin superfamily protein