Recombinant Porcine PD-1 Fc Chimera Protein, CF

Catalog #: 10484-PD Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
10484-PD-050

Recombinant Porcine PD-1 Fc Chimera Protein Binding Activity

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Porcine PD-1 Fc Chimera Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. When Recombinant Porcine PD‑1 Fc Chimera (Catalog # 10484-PD) is immobilized at 1 µg/mL (100 µL/well), Recombinant Human PD‑L1/B7‑H1 Fc Chimera (Catalog # 156-B7) that produces a 50% optimal binding response is found to be 0.03-0.3 µg/mL.

Source

Chinese Hamster Ovary cell line, CHO-derived porcine PD-1 protein

Porcine PD-1 (Leu25-Gln169) Accession # NP_001191308.1	IEGRMD	Human IgG₁ (Pro100-Lys330)
N-terminus		C-terminus

Accession #

NP_001191308.1

N-terminal Sequence
Analysis

Leu25

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

43 kDa

SDS-PAGE

60-75 kDa, under reducing conditions

Product Datasheets

10484-PD

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10484-PD

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity

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When Recombinant Porcine PD‑1 Fc Chimera (10484-PD) is immobilized at 1 µg/mL (100 µL/ well), Recombinant Human PD-L1/B7‑H1 Fc Chimera (156-B7) that produces a 50% optimal binding response is found to be 0.03‑0.3 µg/mL

SDS-PAGE

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2 μg/lane of Recombinant Porcine PD-1 Fc Chimera Protein (10484-PD) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 60-75 kDa and 120-150 kDa, respectively.

Reconstitution Calculator

Background: PD-1

Programmed Death-1 receptor (PD-1), also known as CD279, is type I transmembrane protein belonging to the CD28 family of immune regulatory receptors (1). Other members of this family include CD28, CTLA-4, ICOS, and BTLA (2-5). PD-1 consists of an extracellular domain (ECD) with one immunoglobulin-like V-type domain, a transmembrane domain, and a cytoplasmic region with two tyrosine resideus. These tyrosine residues form the immunoreceptor tyrosine-based inhbitory motif (ITIM) and immunoreceptor tyrosine-based switch motif (ITSM) tha are important for mediating PD-1 signaling. The extracellular domain (ECD) of porcine PD-1 shares 68% and 60% amino acid sequence identity with the human and rat PD-1, respectively. PD-1 acts as a monomeric receptor and interacts in a 1:1 stoichiometric ratio with its ligands PD-L1 (B7-H1) and PD-L2 (B7-DC) (6, 7). PD-1 is expressed on activated T cells, B cells, monocytes, and dendritic cells while PD-L1 expression is constitutive on the same cells and on non-hematopoietic cells such as lung endothelial cells and hepatocytes (8, 9). Ligation of PD-L1 with PD-1 induces co-inhibitory signals on T cells promoting their apoptosis, anergy, and functional exhaustion (10). Thus, the PD-1: PD-L1 interaction is a key regulator of the threshold of immune response and peripheral immune tolerance (11). Blockade of the PD-1: PD-L1 interaction by either antibodies or genetic manipulation accelerates tumor eradication and shows potential for improving cancer immunotherapy (12-14).

References