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Recombinant Rat CNTF Protein

Catalog #: 557-NT
37 Citations 1 Review Datasheet / COA / SDS

Carrier Free

Catalog # Availability Size / Price Qty
557-NT-010/CF
557-NT-050/CF

With Carrier

Catalog # Availability Size / Price Qty
557-NT-010
557-NT-050
R&D Systems Recombinant Proteins and Enzymes
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Product Details
Citations (37)
FAQs
Reviews (1)
Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Rat CNTF Protein Summary

Product Specifications

Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The ED50 for this effect is 3‑15 ng/mL. Measured by its ability to support the survival and stimulate neurite outgrowth of dissociated chick embryonic dorsal root ganglia (DRG) neurons. Davies, A.M. (1989) in Neurotrophic Factor Bioassay Using Dissociated Neurons, Nerve Growth Factor. Rush, R.A. (eds): John Willey and Sons, Ltd. 95. The ED50 for this effect is 0.1-0.3 ng/mL.
Source
E. coli-derived rat CNTF protein
Ala2-Met200
Accession #
P20294.1
N-terminal Sequence
Analysis
Ala2
Predicted Molecular Mass
22.8 kDa

Product Datasheets

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557-NT (with carrier)

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COA
SDS

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557-NT/CF (carrier free)

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COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

557-NT

Formulation Lyophilized from a 0.2 μm filtered solution in Tris, NaCl, TCEP, EDTA, CHAPS and Trehalose with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

557-NT/CF

Formulation Lyophilized from a 0.2 μm filtered solution in Tris, NaCl, TCEP, EDTA, CHAPS and Trehalose.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CNTF

Ciliary neurotrophic factor (CNTF) is a polypeptide initially purified from chick embryo ocular tissue and identified as a trophic factor for embryonic chick ciliary parasympathetic neurons in culture. Subsequent studies have demonstrated that CNTF is a survival factor for additional neuronal cell types including: dorsal root ganglion sensory neurons, sympathetic ganglion neurons, embryonic motor neurons, major pelvic ganglion neurons and hippocampal neurons. CNTF has also been shown to prevent the degeneration of motor axons after axotomy.

The cDNA for CNTF encodes a 200 amino acid residue polypeptide that lacks a signal sequence. CNTF is highly conserved across species and exhibits cross-species activities. Human and rat CNTF share approximately 83% homology in their protein sequence. CNTF is structurally related to IL‑6, IL-11, LIF, and OSM. All of these four helix bundle cytokines share gp130 as a signal-transducing subunit in their receptor complexes. For a list of CNTF references, see the R&D Systems’ cytokine catalog.

Long Name
Ciliary Neurotrophic Factor
Entrez Gene IDs
1270 (Human); 12803 (Mouse); 25707 (Rat)
Alternate Names
ciliary neurotrophic factor; CNTF; HCNTF

Citations for Recombinant Rat CNTF Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

37 Citations: Showing 1 - 10
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  1. A phenotypic screening platform for identifying chemical modulators of astrocyte reactivity
    Authors: Clayton, BLL;Kristell, JD;Allan, KC;Cohn, EF;Karl, M;Jerome, AD;Garrison, E;Maeno-Hikichi, Y;Sturno, AM;Kerr, A;Shick, HE;Sepeda, JA;Freundt, EC;Sas, AR;Segal, BM;Miller, RH;Tesar, PJ;
    Nature neuroscience
    Species: Murine polyomavirus strain A3
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Pervasive environmental chemicals impair oligodendrocyte development
    Authors: EF Cohn, BLL Clayton, M Madhavan, S Yacoub, Y Federov, K Paul-Fried, TJ Shafer, PJ Tesar
    bioRxiv : the preprint server for biology, 2023-02-12;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Midkine expression by stem-like tumor cells drives persistence to mTOR inhibition and an immune-suppressive microenvironment
    Authors: Y Tang, DJ Kwiatkowsk, EP Henske
    Nature Communications, 2022-08-26;13(1):5018.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  4. Oligodendrocyte differentiation alters tRNA modifications and codon optimality-mediated mRNA decay
    Authors: S Martin, KC Allan, O Pinkard, T Sweet, PJ Tesar, J Coller
    Nature Communications, 2022-08-25;13(1):5003.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  5. Latrotoxin-Induced Neuromuscular Junction Degeneration Reveals Urocortin 2 as a Critical Contributor to Motor Axon Terminal Regeneration
    Authors: G D'Este, M Stazi, S Negro, A Megighian, F Lista, O Rossetto, C Montecucco, M Rigoni, M Pirazzini
    International Journal of Molecular Sciences, 2022-01-21;23(3):.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  6. Non-canonical Targets of HIF1a Impair Oligodendrocyte Progenitor Cell Function
    Authors: KC Allan, LR Hu, MA Scavuzzo, AR Morton, AS Gevorgyan, EF Cohn, BLL Clayton, IR Bederman, S Hung, CF Bartels, M Madhavan, PJ Tesar
    Cell Stem Cell, 2020-10-21;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  7. The palmitoyl acyltransferases ZDHHC5 and ZDHHC8 are uniquely present in DRG axons and control retrograde signaling via the Gp130/JAK/STAT3 pathway
    Authors: KM Collura, J Niu, SS Sanders, A Montersino, SM Holland, GM Thomas
    J. Biol. Chem., 2020-09-21;0(0):.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  8. CNTF Prevents Development of Outer Retinal Neovascularization Through Upregulation of CxCl10
    Authors: F Bucher, E Aguilar, KV Marra, J Rapp, J Arnold, S Diaz-Aguil, C Lange, H Agostini, G Schlunck, A Stahl, M Friedlande
    Invest. Ophthalmol. Vis. Sci., 2020-08-03;61(10):20.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  9. Spinal Motoneuron TMEM16F Acts at C-boutons to Modulate Motor Resistance and Contributes to ALS Pathogenesis
    Authors: C Soulard, C Salsac, K Mouzat, C Hilaire, J Roussel, A Mezghrani, S Lumbroso, C Raoul, F Scamps
    Cell Rep, 2020-02-25;30(8):2581-2593.e7.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  10. Roles of Collagen XXV and Its Putative Receptors PTPsigma/delta in Intramuscular Motor Innervation and Congenital Cranial Dysinnervation Disorder
    Authors: H Munezane, H Oizumi, T Wakabayash, S Nishio, T Hirasawa, T Sato, A Harada, T Yoshida, T Eguchi, Y Yamanashi, T Hashimoto, T Iwatsubo
    Cell Rep, 2019-12-24;29(13):4362-4376.e6.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Cell Culture
  11. IL-22 Binding Protein Promotes the Disease Process in Multiple Sclerosis
    Authors: H Lindahl, AO Guerreiro-, SK Bedri, M Linnerbaue, M Lindén, N Abdelmagid, K Tandre, C Hollins, L Irving, C Glover, C Jones, L Alfredsson, L Rönnblom, I Kockum, M Khademi, M Jagodic, T Olsson
    J. Immunol., 2019-07-10;0(0):.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Cell Culture
  12. The Parkinson's disease-linked Leucine-rich repeat kinase 2 (LRRK2) is required for insulin-stimulated translocation of GLUT4
    Authors: N Funk, M Munz, T Ott, K Brockmann, A Wenninger-, R Kühn, D Vogt-Weise, F Giesert, W Wurst, T Gasser, S Biskup
    Sci Rep, 2019-03-14;9(1):4515.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  13. MAP4K4 Activation Mediates Motor Neuron Degeneration in Amyotrophic Lateral Sclerosis
    Authors: C Wu, ME Watts, LL Rubin
    Cell Rep, 2019-01-29;26(5):1143-1156.e5.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  14. Neuroprotective Effects of FGF2 and Minocycline in Two Animal Models of Inherited Retinal Degeneration
    Authors: J Di Pierdom, R Scholz, FJ Valiente-S, MC Sánchez-Mi, M Vidal-Sanz, T Langmann, M Agudo-Barr, D García-Ayu, MP Villegas-P
    Invest. Ophthalmol. Vis. Sci., 2018-09-04;59(11):4392-4403.
    Species: Rat
    Sample Types: In Vivo
    Applications: In Vivo
  15. Inhibiting p38 MAPK alpha rescues axonal retrograde transport defects in a mouse model of ALS
    Authors: KL Gibbs, B Kalmar, ER Rhymes, AD Fellows, M Ahmed, P Whiting, CH Davies, L Greensmith, G Schiavo
    Cell Death Dis, 2018-05-22;9(6):596.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  16. Retinal de novo lipogenesis coordinates neurotrophic signaling to maintain vision
    Authors: R Rajagopal, S Zhang, X Wei, T Doggett, S Adak, J Enright, V Shah, G Ling, S Chen, J Yoshino, FF Hsu, CF Semenkovic
    JCI Insight, 2018-01-11;3(1):.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: Bioassay
  17. Novel combinatorial screening identifies neurotrophic factors for selective classes of motor neurons
    Authors: S Schaller, D Buttigieg, A Alory, A Jacquier, M Barad, M Merchant, D Gentien, P de la Gran, G Haase
    Proc. Natl. Acad. Sci. U.S.A, 2017-03-07;114(12):E2486-E2493.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  18. Embryonic Stem Cell-Derived Neurons Grown on Multi-Electrode Arrays as a Novel In vitro Bioassay for the Detection of Clostridium botulinum Neurotoxins
    Authors: SP Jenkinson, D Grandgirar, M Heidemann, A Tscherter, MA Avondet, SL Leib
    Front Pharmacol, 2017-02-23;8(0):73.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  19. CNTF Attenuates Vasoproliferative Changes Through Upregulation of SOCS3 in a Mouse-Model of Oxygen-Induced Retinopathy
    Invest Ophthalmol Vis Sci, 2016-08-01;57(10):4017-26.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  20. A system to study mechanisms of neuromuscular junction development and maintenance
    Development, 2016-05-25;0(0):.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: Bioassay
  21. Novel Kidins220/ARMS Splice Isoforms: Potential Specific Regulators of Neuronal and Cardiovascular Development.
    Authors: Schmieg N, Thomas C, Yabe A, Lynch D, Iglesias T, Chakravarty P, Schiavo G
    PLoS ONE, 2015-06-17;10(6):e0129944.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  22. Signal transducer and activator of transcription-3 maintains the stemness of radial glia at mid-neurogenesis.
    Authors: Hong S, Song M
    J Neurosci, 2015-01-21;35(3):1011-23.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  23. Bicaudal-D1 regulates the intracellular sorting and signalling of neurotrophin receptors.
    Authors: Terenzio M, Golding M, Russell M, Wicher K, Rosewell I, Spencer-Dene B, Ish-Horowicz D, Schiavo G
    EMBO J, 2014-06-11;33(14):1582-98.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  24. Jak/Stat signaling stimulates zebrafish optic nerve regeneration and overcomes the inhibitory actions of Socs3 and Sfpq.
    Authors: Elsaeidi, Fairouz, Bemben, Michael, Zhao, Xiao-Fen, Goldman, Daniel
    J Neurosci, 2014-02-12;34(7):2632-44.
    Species: Zebrafish
    Sample Types: Whole Tissue
    Applications: Bioassay
  25. Opposite effects of a high-fat diet and calorie restriction on ciliary neurotrophic factor signaling in the mouse hypothalamus.
    Authors: Severi I, Perugini J, Mondini E, Smorlesi A, Frontini A, Cinti S, Giordano A
    Front Neurosci, 2013-12-27;7(0):263.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  26. Ciliary neurotrophic factor promotes motor reinnervation of the musculocutaneous nerve in an experimental model of end-to-side neurorrhaphy.
    Authors: Dubovy P, Raska O, Klusakova I, Stejskal L, Celakovsky P, Haninec P
    BMC Neurosci, 2011-06-22;12(0):58.
    Species: Rat
    Sample Types: In Vivo
    Applications: In Vivo
  27. Specification of transplantable astroglial subtypes from human pluripotent stem cells.
    Authors: Krencik R, Weick JP, Liu Y, Zhang ZJ, Zhang SC
    Nat. Biotechnol., 2011-05-22;29(6):528-34.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  28. Chronic intravitreous infusion of ciliary neurotrophic factor modulates electrical retinal stimulation thresholds in the RCS rat.
    Authors: Kent TL, Glybina IV, Abrams GW, Iezzi R
    Invest. Ophthalmol. Vis. Sci., 2008-01-01;49(1):372-9.
    Species: Rat
    Sample Types: In Vivo
    Applications: In Vivo
  29. Intraocular CNTF reduces vision in normal rats in a dose-dependent manner.
    Authors: McGill TJ, Prusky GT, Douglas RM, Yasumura D, Matthes MT, Nune G, Donohue-Rolfe K, Yang H, Niculescu D, Hauswirth WW, Girman SV, Lund RD, Duncan JL, Lavail MM
    Invest. Ophthalmol. Vis. Sci., 2007-12-01;48(12):5756-66.
    Species: Rat
    Sample Types: In Vivo
    Applications: In Vivo
  30. The expression of L-type voltage-gated calcium channels in retinal photoreceptors is under circadian control.
    Authors: Ko ML, Liu Y, Dryer SE, Ko GY
    J. Neurochem., 2007-08-07;103(2):784-92.
    Species: Chicken
    Sample Types: Whole Cells
    Applications: Bioassay
  31. Microglia-derived interleukin-6 and leukaemia inhibitory factor promote astrocytic differentiation of neural stem/progenitor cells.
    Authors: Nakanishi M, Niidome T, Matsuda S, Akaike A, Kihara T, Sugimoto H
    Eur. J. Neurosci., 2007-02-01;25(3):649-58.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  32. gp130 signaling in proopiomelanocortin neurons mediates the acute anorectic response to centrally applied ciliary neurotrophic factor.
    Authors: Janoschek R, Plum L, Koch L, Munzberg H, Diano S, Shanabrough M, Muller W, Horvath TL, Bruning JC
    Proc. Natl. Acad. Sci. U.S.A., 2006-07-03;103(28):10707-12.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  33. Protective effects of neurotrophic factors on tumor necrosis factor-related apoptosis-inducing ligand (TRAIL)-mediated apoptosis of murine adrenal chromaffin cell line tsAM5D.
    Authors: Murata T, Tsuboi M, Hikita K, Kaneda N
    J. Biol. Chem., 2006-06-13;281(32):22503-16.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  34. NT-3 and CNTF exert dose-dependent, pleiotropic effects on cells in the immature dorsal root ganglion: neuregulin-mediated proliferation of progenitor cells and neuronal differentiation.
    Authors: Hapner SJ, Nielsen KM, Chaverra M, Esper RM, Loeb JA, Lefcort F
    Dev. Biol., 2006-05-19;297(1):182-97.
    Species: Avian
    Sample Types: Whole Cells
    Applications: Bioassay
  35. Novel regulators of kidney development from the tips of the ureteric bud.
    Authors: Schmidt-Ott KM, Yang J, Chen X, Wang H, Paragas N, Mori K, Li JY, Lu B, Costantini F, Schiffer M, Bottinger E, Barasch J
    J. Am. Soc. Nephrol., 2005-05-25;16(7):1993-2002.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: Bioassay
  36. Different aspects of gliosis in retinal Muller glia can be induced by CNTF, insulin, and FGF2 in the absence of damage.
    Authors: Fischer AJ, Omar G, Eubanks J, McGuire CR, Dierks BD, Reh TA
    Mol. Vis., 2004-12-22;10(0):973-86.
    Species: Chicken
    Sample Types: In Vivo
    Applications: In Vivo
  37. Ciliary neurotrophic factor improves nerve conduction and ameliorates regeneration deficits in diabetic rats.
    Authors: Mizisin AP, Vu Y, Shuff M, Calcutt NA
    Diabetes, 2004-07-01;53(7):1807-12.
    Species: Rat
    Sample Types: In Vivo
    Applications: In Vivo

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Recombinant Rat CNTF Protein
By Michelle Chen on 05/10/2017
Application: CellProlif