Recombinant Rat Notch-1 Fc Chimera Protein, CF

Catalog #: 1057-TK
4 Citations Datasheet / COA / SDS

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1057-TK-050

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Citations (4)

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Rat Notch-1 Fc Chimera Protein, CF Summary

Product Specifications

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. Immobilized rrNotch-1/Fc Chimera can bind rrJagged-1/Fc Chimera with an apparent K_D<5 nM.

Source

Mouse myeloma cell line, NS0-derived rat Notch-1 protein

Rat Notch-1 Arg20 - Glu488 (Ala208Thr & Asp334Glu) Accession #Q07008.2	IEGRMD	Human IgG₁ (Pro100 - Lys330)
N-terminus		C-terminus

Accession #

Q07008.2

N-terminal Sequence
Analysis

Arg20

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

76 kDa (monomer)

SDS-PAGE

117 kDa, reducing conditions

Product Datasheets

1057-TK

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1057-TK

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 500 μg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: Notch-1

Rat Notch-1 is a 300 kDa, type I transmembrane glycoprotein involved in a number of early-event developmental processes (1). In both vertebrates and invertebrates, Notch signaling is important for specifying cell fates and for defining boundaries between different cell types. The molecule is synthesized as a 2531 amino acid (aa) precursor that contains an 18 aa signal sequence, a 1705 aa extracellular region, a 23 aa transmembrane (TM) segment and a 785 aa cytoplasmic domain (2). The large Notch-1 extracellular domain has 36 EGF-like repeats followed by three notch/Lin-12 repeats. Of the 36 EGF-like repeats, the 11^th and 12^th EGF-like repeats have been shown to be both necessary and sufficient for binding the ligands Delta and Serrate, in Drosophila (3). The Notch-1 cytoplasmic domain contains six ankyrin repeats, a glutamine-rich domain and a PEST sequence. The Notch-1 receptor undergoes post-translational proteolytic cleavage by a furin-like enzyme to form a heterodimer of the 1635 aa ligand binding extracellular region and the 877 aa transmembrane protein (4). Upon ligand binding, additional sequential proteolysis by TNF-converting enzyme and the Presenilin-dependent gamma -secretase results in the release of the Notch intracellular domain (NCID) which translocates into the nucleus where it functions as a transcription activator to initiate transcription of Notch-responsive genes (5). An alternative Notch signaling pathway that is mediated by the full-length form of Notch that has not been cleaved by the furin-like enzyme has also been reported (6). The rat Notch-1 extracellular domain shows 86% and 97% aa identity to human and mouse Notch-1 extracellular domains respectively. It also exhibits 56% and 50% aa identity with rat Notch-2 and Notch-3 extracellular domains, respectively.

References

Weinmaster, G. (2000) Curr. Opin. Genet. Dev. 10:363.
Weinmaster, G. et al. (1991) Development 113:199.
Rebay, I. et al. (1991) Cell 67:687.
Rogeat, F. et al. (1998) Proc. Natl. Acad. Sci. USA 95:8108.
Mumm, J.S. and R. Kopan (2000) Dev. Biol. 228:151.
Bush, G. et al. (2001) Dev. Biol. 229:494.

Entrez Gene IDs

4851 (Human); 25496 (Rat)

Alternate Names

EC 2.1.2.11; EC 3.4.21.68; hN1; Notch (Drosophila) homolog 1 (translocation-associated); notch 1Notch homolog 1, translocation-associated (Drosophila); Notch homolog 1, translocation-associated; Notch1; Notch-1; TAN1; TAN1neurogenic locus notch homolog protein 1; Translocation-associated notch protein TAN-1

Related Research Areas

Citations for Recombinant Rat Notch-1 Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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Selective regulation of Notch ligands during angiogenesis is mediated by vimentin
Authors: D Antfolk, M Sjöqvist, F Cheng, K Isoniemi, CL Duran, A Rivero-Mul, C Antila, R Niemi, S Landor, CVC Bouten, KJ Bayless, JE Eriksson, CM Sahlgren
Proc. Natl. Acad. Sci. U.S.A., 2017-05-22;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay
Delta/Notch-Like EGF-Related Receptor (DNER) Is Not a Notch Ligand
PLoS ONE, 2016-09-13;11(9):e0161157.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
Self-renewal of embryonic-stem-cell-derived progenitors by organ-matched mesenchyme.
Authors: Sneddon, Julie B, Borowiak, Malgorza, Melton, Douglas
Nature, 2012-10-07;491(7426):765-8.
Species: Human
Sample Types: Whole Cells
Applications: Cell Culture
Evidence for a role for notch signaling in the cytokine-dependent survival of activated T cells.
Authors: Bheeshmachar G, Purushotaman D, Sade H, Gunasekharan V, Rangarajan A, Sarin A
J. Immunol., 2006-10-15;177(8):5041-50.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay