Recombinant Rat PD-1 Fc Chimera Protein, CF

Catalog #: 9814-PD Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
9814-PD-100

Recombinant Rat PD-1 Fc Chimera Protein Binding Activity

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Rat PD-1 Fc Chimera Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. When Recombinant Rat PD-L1/B7-H1 Fc Chimera (Catalog # 9835-B7) is coated at 1 μg/mL (100 μL/well), the concentration of Recombinant Rat PD-1 Fc Chimera (Catalog # 9814-PD) that produces 50% optimal binding response is typically 0.1-0.6 μg/mL.

Source

Mouse myeloma cell line, NS0-derived rat PD-1 protein

Rat PD-1 (Leu25-Gln167) Accession # NP_001100397	IEGRMDP	Mouse IgG_2a (Glu98-Lys330)
N-terminus		C-terminus

Accession #

NP_001100397

N-terminal Sequence
Analysis

Leu25

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

43 kDa

SDS-PAGE

57-70 kDa, reducing conditions

Product Datasheets

9814-PD

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9814-PD

Formulation	Lyophilized from a 0.2 μm filtered solution in Tris, NaCl and EDTA with Sucrose and Mannitol.
Reconstitution	Reconstitute at 500 μg/mL in water.
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	12 months from date of receipt, ≤ -20 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity

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When Recombinant Rat PD-L1/B7-H1 Fc Chimera (Catalog # 9835-B7) is coated at 1 µg/mL, 100 µL/well, Recombinant Rat PD-1 Fc Chimera (Catalog # 9814-PD) binds with an ED₅₀ of 0.1-0.6 µg/mL.

SDS-PAGE

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2 μg/lane of Recombinant Rat PD‑1 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® blue staining, showing bands at 57-70 kDa and 110-140 kDa, respectively.

Reconstitution Calculator

Background: PD-1

Programmed Death-1 receptor (PD-1), also known as CD279, is type I transmembrane protein belonging to the CD28 family of immune regulatory receptors (1). Other members of this family include CD28, CTLA-4, ICOS, and BTLA (2-5). Mature rat PD-1 consists of a 142 amino acid (aa) extracellular domain (ECD), a 28 aa transmembrane segment, and a 95 aa cytoplasmic domain. The ECD of rat PD-1 shares 67% and 87% aa sequence identity with the human and mouse PD-1, respectively. The cytoplasmic tail contains two tyrosine residues that form the immunoreceptor tyrosine-based inhibitory motif (ITIM) and immunoreceptor tyrosine-based switch motif (ITSM) that are important for mediating PD-1 signaling. PD-1 acts as a monomeric receptor and interacts in a 1:1 stoichiometric ratio with its ligands PD-L1 (B7-H1) and PD-L2 (B7-DC) (6, 7). PD‑1 is expressed on activated T cells, B cells, monocytes, and dendritic cells while PD-L1 expression is constitutive on the same cells and on non-hematopoietic cells such as lung endothelial cells and hepatocytes (8, 9). Ligation of PD-L1 with PD-1 induces co-inhibitory signals on T cells promoting their apoptosis, anergy, and functional exhaustion (10). Thus, the PD-1: PD-L1 interaction is a key regulator of the threshold of immune response and peripheral immune tolerance (11). Blockade of the PD-1: PD-L1 interaction by either antibodies or genetic manipulation accelerates tumor eradication and shows potential for improving cancer immunotherapy (12-14).

References