Ro 5-3335
Chemical Name: 7-Chloro-1,3-dihydro-5-(1H-pyrrol-2-yl)-2H-1,4-benzodiazepin-2-one
Purity: ≥98%
Biological Activity
Ro 5-3335 is a core binding factor (CBF) inhibitor; preferentially kills human leukemia cell lines with CBF fusion proteins (IC50 = 1.1 μM). Represses RUNX1/CBFβ-dependent transactivation in reporter assays and inhibits transcriptional regulation by RUNX1 and CBFβ. Reduces leukemia burden in a mouse model. Attenuates RUNX1-dependent hematopoiesis in zebrafish embryos. Also a Tat antagonist; inhibits HIV-1 replication in vitro.Technical Data
The technical data provided above is for guidance only.
For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Background References
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The human immunodeficiency virus type 1 Tat antagonist, Ro 5-3335, predominantly inhibits transcription initiation from the viral promoter.
Cupelli et al.
J.Virol., 1995;69:2640 -
Identification of benzodiazepine Ro5-3335 as an inhibitor of CBF leukemia through quantitative high throughput screen against RUNX1-CBF� interaction.
Cunningham et al.
Proc.Natl.Acad.Sci.USA, 2012;109:14592 -
RUNX1/AML1: a central player in hematopoiesis.
Okuda et al.
Int. J. Hematol., 2001;74:252
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Citations for Ro 5-3335
The citations listed below are publications that use Tocris products. Selected citations for Ro 5-3335 include:
2 Citations: Showing 1 - 2
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LY6D marks pre-existing resistant basosquamous tumor subpopulations.
Authors: Jean Y Et al.
Nat Commun 2022;13:7520
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Runt-related Transcription Factor 1 (RUNX1) Binds to p50 in Macrophages and Enhances TLR4-triggeredInflammation and Septic Shock.
Authors: Luo Et al.
Journal of Biological Chemistry 2016;
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