Sample Activation Kit 1
Sample Activation Kit 1 Summary
INTENDED USE & DESCRIPTION
Sample Activation Kit 1 (Part # 993078) offers convenient, premixed reagents for the activation of samples containing TGF-beta. The kit contains 3 vials of 1N HCL (Part # 895094, 10 mL/vial) and 3 vials of 1.2N NaOH/0.5M HEPES (Part # 895096, 10 mL/vial) for activation of samples in all of R&D Systems’ TGF-beta related products, including DuoSet® ELISA Development Systems and Quantikine® ELISAs, the Luminex® Performance Assays.
STORAGE & STABILITY
Store unopened at room temperature. Do not use past kit expiration. Stable for up to 30 days at room temperature after opening provided this is within the expiration date of the kit. Contains no preservatives.
PROCEDURE & EXPECTED VALUES
Follow activation procedure provided with each application. The kit contains reagents in quantities sufficient for 5 ELISA plates worth of samples.
TECHNICAL HINTS & LIMITATIONS OF THE PROCEDURE
- Caution: May cause skin, eye, and respiratory irritation. Avoid breathing fumes. Wear protective gloves, clothing, and eye and face protection. Wash hands thoroughly after handling. Please refer to the MSDS on our website prior to use.
Specifications
Product Datasheets
Citations for Sample Activation Kit 1
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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A TGFbetaR inhibitor represses keratin-7 expression in 3D cultures of human salivary gland progenitor cells
Authors: EW Fowler, EJ van Venroo, RL Witt, X Jia
Scientific Reports, 2022-09-02;12(1):15008. 2022-09-02
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Exposure modality influences viral kinetics but not respiratory outcome of COVID-19 in multiple nonhuman primate species
Authors: AC Fears, BJ Beddingfie, NR Chirichell, N Slisarenko, SZ Killeen, RK Redmann, K Goff, S Spencer, B Picou, N Golden, CC Midkiff, DJ Bush, LM Branco, ML Boisen, H Gao, DC Montefiori, RV Blair, LA Doyle-Meye, K Russell-Lo, NJ Maness, CJ Roy
PloS Pathogens, 2022-07-05;18(7):e1010618. 2022-07-05
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Peripheral blood CD4posCD25posFoxP3pos cells and inflammatory cytokines as biomarkers of response in rheumatoid arthritis patients treated with CTLA4-Ig
Authors: G Elisa, B Tolusso, L Petricca, C Di Mario, MR Gigante, G Ferracciol, S Alivernini
Arthritis Research & Therapy, 2022-06-15;24(1):143. 2022-06-15
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Role of Betaglycan in TGF-beta Signaling and Wound Healing in Human Endometriotic Epithelial Cells and in Endometriosis
Authors: AN Mwaura, MA Riaz, JB Maoga, E Mecha, COA Omwandho, G Scheiner-B, I Meinhold-H, L Konrad
Biology, 2022-03-26;11(4):. 2022-03-26
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Fatty acid nitroalkene reversal of established lung fibrosis
Authors: A Koudelka, V Cechova, M Rojas, N Mitash, A Bondonese, C St Croix, MA Ross, BA Freeman
Redox Biology, 2021-12-29;50(0):102226. 2021-12-29
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Palladin isoforms 3 and 4 regulate cancer-associated fibroblast pro-tumor functions in pancreatic ductal adenocarcinoma
Authors: JI Alexander, DB Vendramini, R Francescon, T Luong, J Franco-Bar, N Shah, JC Gardiner, E Nicolas, KS Raghavan, E Cukierman
Scientific Reports, 2021-02-15;11(1):3802. 2021-02-15
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Age-induced accumulation of methylmalonic acid promotes tumour progression
Authors: AP Gomes, D Ilter, V Low, JE Endress, J Fernández-, A Rosenzweig, T Schild, D Broekaert, A Ahmed, M Planque, I Elia, J Han, C Kinzig, E Mullarky, AP Mutvei, J Asara, R de Cabo, LC Cantley, N Dephoure, SM Fendt, J Blenis
Nature, 2020-08-19;0(0):. 2020-08-19
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Inhibition of Myostatin Reduces Collagen Deposition in a Mouse Model of Oculopharyngeal Muscular Dystrophy (OPMD) With Established Disease
Authors: P Harish, L Forrest, S Herath, G Dickson, A Malerba, L Popplewell
Front Physiol, 2020-03-05;11(0):184. 2020-03-05
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A-Kinase Anchoring Proteins Diminish TGF-&beta1/Cigarette Smoke-Induced Epithelial-To-Mesenchymal Transition
Authors: H Zuo, M Trombetta-, IH Heijink, CHTJ van der Ve, L Hesse, KN Faber, WJ Poppinga, H Maarsingh, VO Nikolaev, AM Schmidt
Cells, 2020-02-03;9(2):. 2020-02-03
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Myoferlin regulates epithelial cancer cell plasticity and migration through autocrine TGF-?1 signaling
Authors: VR Barnhouse, JL Weist, VC Shukla, SN Ghadiali, DA Kniss, JL Leight
Oncotarget, 2018-04-10;9(27):19209-19222. 2018-04-10
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Average Rating: 5 (Based on 4 Reviews)
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Used this with DY243 and the ancillary kit. Very recommended
We use this in our lab along with the mouse TGF-beta ELISA kit. Activates the samples, straightforward to use, will yield enough for multiple plates.
20uL of rat plasma was acid activated with 10uL of 1N HCl for 10 mins at RT. Samples were neutralized with 10uL of 1.2N NaOH. non-acid treated plasma was tested at a 1:10 dilution and acid treated samples at 1:20.
