Simple Plex Human CXCL12/SDF-1 alpha Cartridge

Catalog #: SPCKB-PS-000299 Datasheet / COA / SDS

Simple Plex Human CXCL12/SDF-1 alpha assay kit for use on Ella instrument. Contains cartridge, sample diluent SD06, and wash buffer

Catalog #	Availability	Size / Price	Qty
SPCKB-PS-000299
SPCKA-PS-000299
SPCKC-PS-000299

Control Products Available

898082: Simple Plex Control for Human CXCL12/SDF-1 alpha

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All CXCL12/SDF-1 alpha Simple Plex Assays

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Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Simple Plex Human CXCL12/SDF-1 alpha Cartridge Summary

Sample Type & Volume Required Per Well*

Platelet-poor EDTA Plasma (5 uL), Platelet-poor Heparin Plasma (5 uL)

Sensitivity

6.57 pg/mL

Assay Range

6.0 - 1,476 pg/mL (Platelet-poor EDTA Plasma, Platelet-poor Heparin Plasma)

Specificity

Natural and recombinant human SDF-1 alpha.

Cross-reactivity

Cross-reactivity observed with 1 or more available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Product Summary

Simple Plex assay for the detection of human SDF-1 alpha in platelet-poor plasma (EDTA/Heparin)

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Platelet-poor EDTA Plasma, Platelet-poor Heparin Plasma

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	38	41
Mean (pg/mL)	27.1	1221	22.7	1049
Standard Deviation	1.56	66.5	3.05	77.4
CV%	5.8	5.4	13.4	7.4

Recovery

Recovery was not necessary as SDF-1 alpha shows natural linearity data from a neat sample to a 1:16 dilution.

Linearity

Samples containing and/or spiked with high concentrations of human SDF-1 alpha were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Scientific Data

N/A

Human SDF-1 alpha Simple Plex Assay Standard Curve

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Human SDF-1 alpha Ella Assay Standard Curve

Product Datasheets

Product Datasheet

COA

SDS

Preparation and Storage

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: CXCL12/SDF-1 alpha

CXCL12/Stromal Cell-derived Factor 1 (SDF-1) is a member of the CXC chemokine ligand superfamily. Six alternatively spliced CXCL12/SDF-1 isoforms have been identified including, CXCL12/SDF-1 alpha, CXCL12/SDF-1 beta, and CXCL12/SDF-1 gamma. CXCL12/SDF-1 alpha is 89 amino acids (aa) in length with a predicted molecular weight of 10 kDa, CXCL12/SDF-1 beta is 93 aa in length with a predicted molecular weight of 10.6 kDa, and CXCL12/SDF-1 gamma is 119 aa in length with a predicted molecular weight of 13.7 kDa. The mouse CXCL12/SDF-1 orthologs share 99% aa sequence identity with the human protein. The alpha and beta isoforms are ubiquitously expressed while the gamma isoform is primarily expressed in the heart. CXCL12/SDF-1 isoforms are chemotactic for T lymphocytes and monocytes. It is cardio protective and has also been shown to hinder CXCR4-mediated HIV infection of T cells.

Entrez Gene IDs:

6387 (Human); 20315 (Mouse); 493806 (Feline)

Alternate Names:

CXCL12 chemokine (C-X-C motif) ligand 12; CXCL12/SDF-1 alpha; IRH; PBSF; SCYB12; SDF1 alpha; SDF1; SDF1a; SDF1B; TLSF; TPAR1