Simple Plex Human Fas/TNFRSF6/CD95 Cartridge

Catalog #: SPCKB-PS-001042 Datasheet / COA / SDS

Simple Plex Human Fas/TNFRSF6/CD95 assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer

Catalog #	Availability	Size / Price	Qty
SPCKB-PS-001042
SPCKA-PS-001042
SPCKC-PS-001042

Control Products Available

898895: Simple Plex Control for Human Fas/TNFRSF6/CD95

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Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Simple Plex Human Fas/TNFRSF6/CD95 Cartridge Summary

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Cell Culture Supernates (10 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL)

Sensitivity

0.83 pg/mL

Assay Range

5.5 - 20,792 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)

Specificity

Natural and recombinant human Fas.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Product Summary

Simple Plex assay for the detection of human Fas in cell culture supernatant (CCS), serum and plasma (EDTA/Heparin).

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	16	16
Mean (pg/mL)	333	16033	330	17111
Standard Deviation	13	1372	22.4	1943
CV%	3.9	8.6	6.8	11.4

Recovery

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Supernates (n=5)	101	99-104

Linearity

Samples containing and/or spiked with high concentrations of human Fas were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Scientific Data

N/A

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Human Fas Simple Plex Assay Standard Curve

Product Datasheets

Product Datasheet

COA

SDS

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Fas/TNFRSF6/CD95

Fas (Fibroblast-associated), also known as Apo-1, CD95, and TNFRSF6, was originally identified as a cell-surface protein that binds to monoclonal antibodies that are cytolytic for various human cell lines. Alternatively spliced cDNAs encoding multiple Fas isoforms, including a soluble form of Fas, have been identified. Fas is highly expressed in epithelial cells, hepatocyes, activated mature lymphocytes, virus-transformed lymphocytes and other tumor cells. Fas expression has also been detected in mouse thymus, liver, heart, lung, kidney and ovary.

Long Name:

Fibroblast-associated

Entrez Gene IDs:

355 (Human); 14102 (Mouse); 246097 (Rat); 102140989 (Cynomolgus Monkey); 493881 (Feline)

Alternate Names:

Apo-1 antigen; Apo-1; apoptosis antigen 1; Apoptosis-mediating surface antigen FAS; APT1; APT1FASTM; CD95 antigen; CD95; CD95ALPS1A; Fas (TNF receptor superfamily, member 6); Fas AMA; Fas antigen; Fas; FAS1; FASLG receptor; TNFRSF6; TNFRSF6member 6; tumor necrosis factor receptor superfamily member 6