Simple Plex Human Growth Hormone Cartridge

Catalog #: SPCKB-PS-000492 Datasheet / COA / SDS

Simple Plex Human Growth Hormone assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer

Catalog #	Availability	Size / Price	Qty
SPCKB-PS-000492
SPCKA-PS-000492
SPCKC-PS-000492

Control Products Available

898092: Simple Plex Control for Human Growth Hormone

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Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Simple Plex Human Growth Hormone Cartridge Summary

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Serum (5 uL), EDTA Plasma (5 uL), Heparin Plasma (5 uL)

Sensitivity

0.67 pg/mL

Assay Range

1.7 - 16,610 pg/mL (Serum, EDTA Plasma, Heparin Plasma)

Specificity

Natural and recombinant human GH.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Product Summary

Simple Plex assay for the detection of human GH in serum and plasma (EDTA/Heparin)

Precision

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision	Inter-Assay Precision
Sample	1	2
n	32	32
Mean (pg/mL)	87.6	4425
Standard Deviation	6.63	397
CV%	7.6	9

Recovery

Recovery was not necessary as GH shows natural linearity data from a neat sample to a 1:16 dilution.

Linearity

Samples containing and/or spiked with high concentrations of human GH were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Scientific Data

N/A

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Human Growth Hormone Simple Plex Assay Standard Curve

Product Datasheets

Product Datasheet

COA

SDS

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Growth Hormone

Growth hormone (GH), also known as somatotropin, is a member of a family of growth factors that includes prolactin, placental lactogens, proliferins, and somatolactin. It is synthesized primarily by somatotropes in the anterior pituitary and is stored in secretory granules.

Entrez Gene IDs:

2688 (Human)

Alternate Names:

GH1; GHB5; GHIGHD1B; GHN; GH-N; GHNGrowth hormone; GH-Nsomatotropin; growth hormone 1Pituitary growth hormone; Growth Hormone; hGH-N; IGHD1A; IGHD2; Somatotropin