Simple Plex Human IFN-gamma (3rd Gen) Cartridge

Name: Simple Plex Human IFN-gamma (3rd Gen) Cartridge
Brand: R&D Systems
SKU: SPCKB-PS-002574
Rating: 5 (1 reviews)

Catalog #: SPCKB-PS-002574 1 Review Datasheet / COA / SDS

Simple Plex Human IFN gamma 3rd gen assay kit contains cartridge, sample diluent SD13, and wash buffer. Part # starting with ST01 ships in 2-3 days. Sample type indicated with PS (Plasma/Serum)

Catalog #	Availability	Size / Price	Qty
SPCKB-PS-002574
SPCKA-PS-002574
SPCKC-PS-002574
ST01B-PS-002574

Control Products Available

899077: Simple Plex Control for Human IFN-gamma (3rd Gen)

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All IFN-gamma Simple Plex Assays

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Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Simple Plex Human IFN-gamma (3rd Gen) Cartridge Summary

Sample Type & Volume Required Per Well*

Cell Culture Supernates (5 uL), Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL), Cerebrospinal Fluid (25 uL)

Sensitivity

0.05 pg/mL

Assay Range

0.2 - 4,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Cerebrospinal Fluid)

Specificity

Natural and recombinant human IFN-gamma.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Product Summary

Simple Plex assay for the detection of human IFN-gamma in cell culture supernatant (CCS), serum, plasma (EDTA/Heparin), and cerebrospinal fluid (CSF) on Ella automated benchtop ELISA.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Cerebrospinal Fluid

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	38	39
Mean (pg/mL)	21.1	1175	23.4	1192
Standard Deviation	1.48	33.4	2.52	99.4
CV%	7	2.8	10.8	8.3

Recovery

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
Cerebrospinal Fluid (n=6)	94	91-98
EDTA Plasma (n=4)	89	88-90
Heparin Plasma (n=4)	90	83-99
Serum (n=4)	95	83-102

Linearity

Samples containing and/or spiked with high concentra- tions of human IFN-gamma were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Scientific Data

N/A

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IFN-gamma Assay Standard Curve

Product Datasheets

Product Datasheet

COA

SDS

Preparation and Storage

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IFN-gamma

IFN-gamma (Interferon-gamma) is the prototype proinflammatory cytokine and is produced by a variety of immune cells under inflammatory conditions, notably by T cells and NK cells. It plays a key role in host defense by promoting the development and activation of Th1 cells, chemoattraction and activation of monocytes and macrophages, upregulation of antigen presentation molecules, and immunoglobulin class switching in B cells. It also exhibits antiviral, antiproliferative, and apoptotic effects. In addition, IFN-gamma functions as an anti-inflammatory mediator by promoting the development of regulatory T cells and inhibiting Th17 cell differentiation. IFN-gamma dimers signal through a receptor complex of two IFN-gamma R1 and two IFN-gamma R2 subunits.

Long Name:

Interferon gamma

Entrez Gene IDs:

3458 (Human); 15978 (Mouse); 25712 (Rat); 396991 (Porcine); 281237 (Bovine); 403801 (Canine); 493965 (Feline)

Alternate Names:

IFG; IFI; IFNG; IFNgamma; IFN-gamma; Immune interferon; interferon gamma; interferon, gamma