Simple Plex Human MAdCAM-1 Cartridge

Catalog #: SPCKB-PS-000510 Datasheet / COA / SDS

Simple Plex Human MAdCAM-1 assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer

Catalog #	Availability	Size / Price	Qty
SPCKB-PS-000510
SPCKA-PS-000510
SPCKC-PS-000510

Control Products Available

898520: Simple Plex Control for Human MAdCAM-1

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Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Simple Plex Human MAdCAM-1 Cartridge Summary

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Cell Culture Supernates (10 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL), Urine (10 uL)

Sensitivity

0.65 pg/mL

Assay Range

3.4 - 12,800 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine)

Specificity

Natural and recombinant human MAdCAM-1.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Product Summary

Simple Plex assay for the detection of human Mucosal Addressin Cell Adhesion Molecule-1 (MAdCAM-1) in cell culture supernatant (CCS), serum, plasma (EDTA/Heparin), and urine.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	53	53
Mean (pg/mL)	129	6645	121	5721
Standard Deviation	11.7	632	13.3	628
CV%	9.1	9.5	11	11

Recovery

Recovery was not necessary as MAdCAM-1 shows natural linearity data from a neat sample to a 1:16 dilution.

Sample Type	Average % Recovery	Range %
Cell Culture Supernates (n=7)	99	87-115

Linearity

Samples containing and/or spiked with high concentrations of human MAdCAM-1 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Scientific Data

N/A

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Human MadCAM-1 Simple Plex Assay Standard Curve

Product Datasheets

Product Datasheet

COA

SDS

Preparation and Storage

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: MAdCAM-1

Mucosal addressin cell adhesion molecule-1 (MAdCAM-1) is an immunoglobulin (Ig) cell adhesion molecule. MAdCAM-1 is involved in lymphocyte homing to mucosal sites and is expressed on high endothelial venules (HEV) of both mesenteric lymph nodes and Peyers patches. It has also been found on sinus-lining cells of the spleen.

Long Name:

Mucosal Addressin Cell Adhesion Molecule 1

Entrez Gene IDs:

8174 (Human); 17123 (Mouse)

Alternate Names:

hMAdCAM-1; MACAM1; MAdCAM1; MAdCAM-1; mucosal addressin cell adhesion molecule 1; mucosal addressin cell adhesion molecule-1; mucosal vascular addressin cell adhesion molecule 1