Simple Plex Human Perforin Cartridge

Catalog #: SPCKB-PS-006982 Datasheet / COA / SDS

Simple Plex Cartridge Kit for quantitation of Perforin. Includes wash buffer and sample diluent. For use with the Ella automated immunoassay system

Catalog #	Availability	Size / Price	Qty
SPCKB-PS-006982
SPCKA-PS-006982
SPCKC-PS-006982

Control Products Available

897018: Simple Plex Control for Human Perforin

Product Details

Supplemental Products

Reviews

Summary Precision Recovery Linearity Product Datasheets Preparation and Storage Background Related Research Areas

Simple Plex Human Perforin Cartridge Summary

Sensitivity

3.14 pg/mL

Assay Range

3.1 - 12,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)

Specificity

Natural and recombinant human Perforin.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Product Summary

Simple Plex assay for the detection of human Perforin in cell culture supernatant (CCS), serum, and plasma (EDTA/Heparin)

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested sixteen times in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	16	16
Mean (pg/mL)	92.9	3901	90.7	4145
Standard Deviation	2.07	74.9	7.41	371
CV%	2.2	1.9	8.2	8.9

Recovery

Recovery was not necessart as Perforin shows natural linearity data from a neat sample to a 1:16 dilution.

Linearity

Samples containing and/or spiked with high concentrations of human Perforin were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Product Datasheets

Product Datasheet

COA

Preparation and Storage

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Perforin

Perforin (PRF1) is a 70 kD cytolytic protein that is the major protein constituent of cytoplasmic granules of cytotoxic T lymphocytes (CTLs) and natural killer (NK) cells. Specifically, perforin enters the target cell's plasma membrand and creates a intercellular pore to mediate targeted cell lysis. PRF1 antibodies are useful tools for lysis research on both CTLs and NK cells.

Long Name:

Perforin 1 (Pore Forming Protein)

Entrez Gene IDs:

5551 (Human); 18646 (Mouse); 50669 (Rat)

Alternate Names:

Cytolysin; FLH2; HPLH2; HPLH2lymphocyte pore forming protein; Lymphocyte pore-forming protein; MGC65093; P1; P1PFN1; perforin 1 (pore forming protein); Perforin; perforin-1; PFP; PFPcytolysin; PRF1