Simple Plex Human Reg3A Cartridge

Catalog #: SPCKB-PS-000524 Datasheet / COA / SDS

Simple Plex Human Reg3A assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer

Catalog #	Availability	Size / Price	Qty
SPCKB-PS-000524
SPCKA-PS-000524
SPCKC-PS-000524

Control Products Available

898241: Simple Plex Control for Human Reg3A

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Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Simple Plex Human Reg3A Cartridge Summary

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Serum (5 uL), EDTA Plasma (5 uL), Heparin Plasma (5 uL)

Sensitivity

1.93 pg/mL

Assay Range

9.8 - 15,000 pg/mL (Serum, EDTA Plasma, Heparin Plasma)

Specificity

Natural and recombinant human Reg3A.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Product Summary

Simple Plex assay for the detection of human Reg3A in serum and plasma (EDTA/Heparin)

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	24	22
Mean (pg/mL)	91	4305	82.1	4011
Standard Deviation	3.36	416	6.38	405
CV%	3.7	9.7	7.8	10.1

Recovery

Recovery was not necessary as Reg3A shows natural linearity data from a neat sample to a 1:16 dilution.

Linearity

Samples containing and/or spiked with high concentrations of human Reg3A were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Scientific Data

N/A

Human Reg3A Simple Plex Assay Standard Curve

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Human Reg3A Ella Assay Standard Curve

Product Datasheets

Product Datasheet

COA

SDS

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Reg3A

The Reg family constitutes a subset of the calcium-dependent C-type lectin superfamily. These small, secreted proteins have been implicated in a range of physiological processes including acting as acute phase reactants, lectins, survival/growth factors for insulin-producing pancreatic beta-cells, neural cells, and epithelial cells of the digestive system. Studies also indicate a role for Reg family members in tumor formation and indicate their potential for use as biomarkers of carcinogenesis.

Reg3A also named lithostathine 3 and pancreatitis-associated protein 2, belongs to the Regenerating gene family of C-type lectins. Reg3 is overexpressed during the acute phase of pancreatitis.

Long Name:

Regenerating Islet-derived IIIa

Entrez Gene IDs:

5068 (Human); 19694 (Mouse); 171162 (Rat)

Alternate Names:

hepatocarcinoma-intestine-pancreas; HIPpancreatitis-associated protein; Human proislet peptide; Lithostathine 3; pancreatic beta cell growth factor; Pancreatitis-associated protein 1; PAP homologous protein; PAP1FLJ18565; PAP2; PAPPAP-H; PBCGF; proliferation-inducing protein 34; proliferation-inducing protein 42; Reg III-alpha; REG3; Reg3A; REG-3-alpha; regenerating islet-derived 3 alpha; regenerating islet-derived protein 3-alpha; Regenerating islet-derived protein III-alpha; REG-III