Simple Plex Mouse IL-12 p70 Cartridge
Simple Plex Mouse IL-12 p70 Cartridge Summary
*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.
Product Summary
Precision
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine
Intra-Assay Precision | Inter-Assay Precision | |||
---|---|---|---|---|
Sample | 1 | 2 | 1 | 2 |
n | 16 | 16 | 51 | 53 |
Mean (pg/mL) | 24 | 1105 | 23.9 | 1088 |
Standard Deviation | 0.67 | 28 | 2.52 | 77.9 |
CV% | 2.8 | 2.5 | 10.6 | 7.2 |
Recovery
Recovery at three different spiked concentrations within the range of the assay was evaluated.
Sample Type | Average % Recovery | Range % |
---|---|---|
Cell Culture Media (n=2) | 104 | 102-106 |
EDTA Plasma (n=4) | 111 | 109-114 |
Heparin Plasma (n=4) | 96 | 91-98 |
Serum (n=4) | 106 | 103-108 |
Urine (n=4) | 90 | 82-93 |
Linearity
Product Datasheets
Preparation and Storage
Background: IL-12 p70
Interleukin 12 (IL-12), also known as natural killer cell stimulatory factor (NKSF) or cytotoxic lymphocyte maturation factor (CLMF), is a heterodimeric pleiotropic cytokine made up of a 40 kDa (p40) subunit and a 35 kDa (p35) subunit. The IL-12 p40 subunit is shared by IL-23, another heterodimeric cytokine that has biological activities similar to, as well as distinct from, IL-12. IL-12 is produced by macrophages and B cells and has been shown to have multiple effects on T cells and natural killer (NK) cells. While mouse IL-12 is active on both human and mouse cells, human IL-12 is not active on mouse cells.
FAQs
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