Name: Simple Plex Mouse Lipocalin-2 Cartridge
Brand: R&D Systems
SKU: SPCKB-MP-007400
Price: 830 USD

Simple Plex Mouse Lipocalin-2 Cartridge Summary

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Bronchoalveolar Lavage Fluid (50 nL), Cell Culture Supernates (50 nL), Serum (25 nL), EDTA Plasma (25 nL), Heparin Plasma (25 nL), Urine (50 nL)

Sensitivity

4.24 Not Assigned

Assay Range

4.2 - 2,584 Not Assigned (Bronchoalveolar Lavage Fluid, Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine)

Specificity

Natural and recombinant mouse Lipocalin-2/NGAL

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Product Summary

Simple Plex assay for the detection of mouse Lipocalin-2 in cell culture supernatant (CCS), serum, plasma (EDTA/Heparin), urine, and bronchoalveolar lavage fluid (BALF)

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Bronchoalveolar Lavage Fluid, Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	16	16
Mean (pg/mL)	18.9	928	17.7	918
Standard Deviation	1.36	42.9	1.86	91.7
CV%	7.2	4.6	10.5	10

Recovery

Recovery was not necessary as Lipocalin-2 shows natural linearity data from a neat sample to a 1:16 dilution.

Linearity

Samples containing and/or spiked with high concentrations of Lipocalin-2 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Product Datasheets

Product Datasheet

COA

SDS

Preparation and Storage

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Lipocalin-2/NGAL

The Lipocalin family comprises a diverse group of mostly secreted soluble proteins that bind hydrophobic ligands and act as transporters, carrying small molecules to specific cells. Lipocalins are related by possessing an 8-stranded beta-barrel structure. Lipocalin-1, also named tear lipocalin (TL), von Ebners gland protein (VEG) and tear pre-albumin, binds a large number of hydrophobic molecules and exhibits cysteine proteinase inhibitor and endonuclear activities. Lipocalin-2, also known as neutrophil gelatinase-associated lipocalin (NGAL), is a component of granules in neutrophils from tissues that are normally exposed to microorganisms and is upregulated during inflammation. Lipocalin-2 can form homodimers and can heterodimerize with the neutrophil gelatinase MMP-9.

Long Name:

Neutrophil Gelatinase-associated Lipocalin

Entrez Gene IDs:

3934 (Human); 16819 (Mouse); 170496 (Rat); 102137149 (Cynomolgus Monkey)

Alternate Names:

24p3; 25 kDa alpha-2-microglobulin-related subunit of MMP-9; HNL; LCN2; lipocalin 2 (oncogene 24p3); lipocalin 2; Lipocalin2; Lipocalin-2; migration-stimulating factor inhibitor; MSFI; neutrophil gelatinase-associated lipocalin; NGAL; NGALlipocalin-2; Oncogene 24p3; p25; Siderocalin; Uterocalin