Simple Plex Rat Lipocalin-2/NGAL Cartridge

Catalog #: SPCKB-RP-000546 Datasheet / COA / SDS

Simple Plex Cartridge Kit for quantitation of Lipocalin-2. Includes wash buffer and sample diluent. For use with the Ella automated immunoassay system

Catalog #	Availability	Size / Price	Qty
SPCKB-RP-000546
SPCKA-RP-000546
SPCKC-RP-000546

Control Products Available

898391: Simple Plex Control for Rat Lipocalin-2/NGAL

Simple Plex Cartridge Kit for 16 samples, 1 analyte for use with Rat Plasma / Serum.

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Summary Precision Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Simple Plex Rat Lipocalin-2/NGAL Cartridge Summary

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Serum (10 uL), EDTA Plasma (10 uL), Urine (10 uL)

Sensitivity

0.69 pg/mL

Assay Range

2.6 - 10,000 pg/mL (Serum, Rat Serum, EDTA Plasma, Rat EDTA Plasma, Urine, Rat Urine)

Specificity

Natural and recombinant rat Lipocalin-2/NGAL.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Product Summary

Simple Plex assay for the detection of rat Lipocalin-2 in serum, plasma (EDTA), and urine.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Serum, EDTA Plasma, Urine

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	21	19
Mean (pg/mL)	48.7	2552	51.5	2683
Standard Deviation	1.87	249	3.88	230
CV%	3.8	9.8	7.5	8.6

Linearity

Samples containing and/or spiked with high concentrations of rat Lipocalin-2 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Scientific Data

N/A

Rat Lipocalin-2/NGAL Simple Plex Assay Standard Curve

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Rat Lipocalin-2/NGAL Ella Assay Standard Curve

Product Datasheets

Product Datasheet

COA

Preparation and Storage

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Lipocalin-2/NGAL

The Lipocalin family comprises a diverse group of mostly secreted soluble proteins that bind hydrophobic ligands and act as transporters, carrying small molecules to specific cells. Lipocalins are related by possessing an 8-stranded beta-barrel structure. Lipocalin-1, also named tear lipocalin (TL), von Ebners gland protein (VEG) and tear pre-albumin, binds a large number of hydrophobic molecules and exhibits cysteine proteinase inhibitor and endonuclear activities. Lipocalin-2, also known as neutrophil gelatinase-associated lipocalin (NGAL), is a component of granules in neutrophils from tissues that are normally exposed to microorganisms and is upregulated during inflammation. Lipocalin-2 can form homodimers and can heterodimerize with the neutrophil gelatinase MMP-9.

Long Name:

Neutrophil Gelatinase-associated Lipocalin

Entrez Gene IDs:

3934 (Human); 16819 (Mouse); 170496 (Rat); 102137149 (Cynomolgus Monkey)

Alternate Names:

24p3; 25 kDa alpha-2-microglobulin-related subunit of MMP-9; HNL; LCN2; lipocalin 2 (oncogene 24p3); lipocalin 2; Lipocalin2; Lipocalin-2; migration-stimulating factor inhibitor; MSFI; neutrophil gelatinase-associated lipocalin; NGAL; NGALlipocalin-2; Oncogene 24p3; p25; Siderocalin; Uterocalin