Viral MCV-type II Chemokine-like Protein Biotinylated Antibody

Catalog #: BAF697 Datasheet / COA / SDS

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BAF697

All MCV-type II Chemokine-like Protein Antibodies

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Viral MCV-type II Chemokine-like Protein Biotinylated Antibody Summary

Species Reactivity

Viral

Specificity

Detects viral MCV-type II Chemokine-like Protein in Western blots. In Western blots, no cross-reactivity with ________ is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant viral MCV-type II Chemokine-like Protein

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Viral MCV-type II Chemokine-like Protein (Catalog # 697-M3)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MCV-type II Chemokine-like Protein

Molluscum contagiosum virus (MCV) is a human cutaneous poxvirus which causes benign proliferative lesions of the skin in normal and immunocompromised individuals. Based on endonuclease cleavage patterns of viral DNA, MCV isolates can be classified into two different types, MCV type I (MCVI) and MCV type II (MCVII). Like other DNA viruses such as poxviruses and herpesviruses, MCV has been shown to encode homologs of cellular immunomodulatory proteins. These viral proteins enable the virus to evade the host immune system and enhance the ability of the virus to propogate and survive.

MC148R2 is a CC chemokine-like protein encoded by a MCV type II gene. MC148R2 cDNA encodes a putative 104 amino acid (aa) protein containing a 24 aa signal peptide that is cleaved to produce an 80 aa mature protein. The mature protein shares 89% homology to the MCV type I chemokine-like protein, MC148R1. Compared to MIP-beta, MC148R2 retains the 4 conserved cysteine residues but has a 5 aa residue deletion at the amino-terminus region which is important for receptor activation. The MCV type I and type 2 proteins do not exhibit chemotactic properties, however these proteins have been shown in vitro to inhibit the monocyte chemotaxis induced by human MIP-1 alpha as well as the binding of radiolabeled I-309 to hCCR8 transfected cells.

Long Name

Molluscum Contagiosum Virus Type II

Alternate Names

MCVtype II; MCV-type II