Zebrafish VEGF Antibody

Catalog #: MAB1247
2 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB1247
MAB1247-SP

Cell Proliferation Induced by VEGF165and Neutralization by Zebrafish VEGF Antibody.

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All VEGF Antibodies

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Citations (2)

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Zebrafish VEGF Antibody Summary

Species Reactivity

Zebrafish

Specificity

Detects zebrafish VEGF in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) VEGF 121 165 206 rhVEGF-B 186 rhVEGF-C, rhVEGF-D, recombinant mouse (rm) VEGF 115 rmVEGF 165 rmVEGF-B 186 rmVEGF-D, or recombinant rat VEGF 164 is observed.

Source

Monoclonal Mouse IgG_2B Clone # 211615

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant zebrafish VEGF 165 Ala24-Arg188 Accession # O73682.1

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Zebrafish VEGF₁₆₅ (Catalog # 1247-ZV)

Neutralization

Measured by its ability to neutralize VEGF₁₆₅-induced proliferation in HUVEC human umbilical vein endothelial cells. The Neutralization Dose (ND₅₀) is typically 0.2-0.5 µg/mL in the presence of 100 ng/mL Recombinant Zebraship VEGF₁₆₅.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization

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Cell Proliferation Induced by VEGF₁₆₅and Neutralization by Zebrafish VEGF Antibody. Recombinant Zebrafish VEGF₁₆₅(Catalog # 1247-ZV) stimulates proliferation in HUVEC human umbilical vein endothelial cells in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Zebrafish VEGF₁₆₅(100 ng/mL) is neutrallized (green line) by increasing concentrations of Mouse Anti-Zebrafish VEGF Monoclonal Antibody (Catalog # MAB1247). The ND₅₀ is typically 0.2-0.5 µg/mL.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

Background: VEGF

Vascular endothelial growth factor (VEGF), also known as vascular permeability factor (VPF) and VEGF-A, is a potent mediator of both angiogenesis and vasculogenesis in the fetus and adult. It is a member of the PDGF family that is characterized by the presence of eight conserved cysteine residues. In human, at least eight alternate splice isoforms of VEGF-A, ranging from 206 amino acids (aa) to 121 aa in length, are known. In zebrafish, two VEGF isoforms, a 165 aa and a 121 aa isoform, have been reported. Mature zebrafish VEGF 165 shares 64%, 62% and 62% aa sequence identity with frog, human, and mouse VEGF 165 respectively. There are two tyrosine kinase receptors for VEGF reported in mammals termed VEGF R1 and VEGF R2/FLK-1. One receptor has been identified in zebrafish (FLK-1), and this may actually represent the orthologue to the early common ancestor for mammalian VEGF R1 and R2. All receptors are type I transmembrane proteins that show seven immunoglobulin-like domains extracellularly and a split kinase domain intracellularly. In addition to the tyrosine kinase receptors, neuropilin-1 (NRP-1) has been reported to be a coreceptor for VEGF binding. It is proposed that the presence of NRP-1 lowers the concentration of VEGF necessary for activation of VEGF R2. NRP-1 has been reported in both zebrafish and human. VEGF regulates multiple biological functions in endothelial cells, including cell proliferation, migration and survival. These functions of VEGF are mediated partly through the induction of nitric oxide and prostacyclin, as well as upregulation of metalloproteinases. Together with other vascular-specific growth factors such as the Angiopoietins, VEGF have separate but complementary roles in angiogenesis and vasculogenesis (1-7).

References

Laing, D. et al. (1998) Biochem. Biophys. Acta 1397:14.
Laing, D. and R. Ge (1998) GenBank Accession # AAC14713.
Laio, W. et al. (1997) Development 124:381.
Lee, P. et al. (2002) Proc. Natl. Acad. Sci. USA 99:10470.
Thurston, G. (2002) J. Anat. 200:575.
Zachary, I. and G. Gliki (2001) Cardiovasc. Res. 49:568.
Robinson, C.J. and S.E. Stringer (2001) J. Cell. Sci. 114:853.

Long Name

Vascular Endothelial Growth Factor

Entrez Gene IDs

7422 (Human); 22339 (Mouse); 83785 (Rat); 281572 (Bovine); 403802 (Canine); 493845 (Feline); 30682 (Zebrafish)

Alternate Names

MVCD1; VAS; vascular endothelial growth factor A; Vascular permeability factor; Vasculotropin; VEGF; VEGFA; VEGF-A; VEGFMGC70609; VPF; VPFvascular endothelial growth factor

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Citations for Zebrafish VEGF Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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CUL2 is required for the activity of hypoxia-inducible factor and vasculogenesis.
Authors: Maeda Y, Suzuki T, Pan X, Chen G, Pan S, Bartman T, Whitsett JA
J. Biol. Chem., 2008-03-27;283(23):16084-92.
Species: Zebrafish
Sample Types: Tissue Homogenates
Applications: Western Blot
Microarray gene expression profiling of cell lines from primary and metastatic tongue squamous cell carcinoma: possible insights from emerging technology.
Authors: Vigneswaran N, Sacks P, Gilcrease M, Zacharias W
J. Oral Pathol. Med., 2005-02-01;34(2):77-86.
Species: Human
Sample Types: Whole Cells
Applications: ICC