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Learn MoreMarginal Zone B Cell Markers
Click on one of the B cell subsets shown in the buttons below to see the human and mouse markers that are commonly used to identify each cell type.
Overview
Marginal zone B cells in mice are located in the outer white pulp of the spleen between the marginal sinus and the red pulp, which allows them to rapidly respond to blood-borne pathogens and participate in both T cell-independent and T cell-dependent immune responses. They develop following migration of transitional 2 (T2) B cells to the spleen, which then mature into either marginal zone B cells or follicular B cells. Marginal zone B cell development is driven by a weak B cell receptor (BCR) signal, along with Notch2 signaling, and BAFF R-mediated canonical NF kappa B signaling. Unlike follicular B cells, marginal zone B cells in mice are immobile, appear to have a long lifespan, and can rapidly mature into plasma cells. In contrast, marginal zone cells in humans are freely recirculating cells that are found in a number of other locations in addition to the spleen. In contrast to follicular B cells, marginal zone B cells express high levels of IgM, and low levels of IgD and CD23/Fc epsilon RII. In addition, both mouse and human marginal zone B cells express high levels of CD1 (CD1d in mice, CD1c in humans), and CD21.
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Data Examples
Detection of Marginal Zone and Follicular B-2 Cells in Mouse Splenocytes. (A) C57BL/6 mouse splenocytes were stained with a PE-conjugated Rat Anti-Mouse CD19 Monoclonal Antibody (Novus Biologicals, Catalog # NBP2-24966) and an Alexa Fluor 488-conjugated Rat Anti-Mouse CD43 Monoclonal Antibody (Novus Biologicals, Catalog # NBP1-43413AF488). CD19+/ CD43– cells were gated. (B) Marginal zone B-2 cells (CD19mid/CD1dhigh/CD23–/CD21high/CD43–) and follicular B-2 cells (CD19mid/CD1dmid/CD23+/CD21low/CD43–) were detected in the CD19+/CD43– population by staining with a fluorochrome-conjugated anti-mouse CD21 monoclonal antibody and an Alexa Fluor® 700-conjugated Rat Anti-Mouse CD1d Monoclonal Antibody (Novus Biologicals, Catalog # NBP1-43461AF700). CD1dmid/CD21low and CD1dhigh/CD21high cells were gated. (C) Marginal zone B-2 cells (CD1dhigh/CD21high) and follicular B-2 cells (CD1dmid/CD21low) were stained for CD21 and CD23 using a fluorochrome-conjugated anti-mouse CD21 monoclonal antibody and an Alexa Fluor® 594-conjugated Rat Anti-Mouse CD23/ Fc epsilon RII Monoclonal Antibody (R&D Systems, Catalog # FAB6900T).
Detection of IgD and IgM on Marginal Zone and Follicular B-2 Cells from Mouse Splenocytes. (A) C57BL/6 mouse splenocytes were stained with an Alexa Fluor® 488-conjugated Rat Anti-Mouse CD43 Monoclonal Antibody (Novus Biologicals, Catalog # NBP1-43413AF488) and a PE-conjugated Rat Anti-Mouse CD19 Monoclonal Antibody (Novus Biologicals, Catalog # NBP2-24966). CD19mid/CD43– cells were gated. (B) Marginal zone B-2 cells (CD19mid/CD1dhigh/CD23–/CD21high/ CD43–) and follicular B-2 cells (CD19mid/CD1dmid/CD23+/CD21low/CD43–) were detected in CD19+/CD43– population by staining with a fluorochrome-conjugated anti-mouse CD21 monoclonal antibody and an Alexa Fluor® 594-conjugated Rat Anti-Mouse CD23/Fc epsilon RII Monoclonal Antibody (R&D Systems, Catalog # FAB6900T). CD21low/CD23+ and CD21high/CD23– cells were gated. (C) Expression of IgM and IgD on marginal zone B-2 (CD19mid/CD23–/CD21high/CD43–/IgMhigh/IgDlow) and follicular B-2 (CD19mid/ CD23+/CD21low/CD43–/IgMlow/IgDhigh) cells was detected using an Alexa Fluor® 647-conjugated rat anti-mouse IgD monoclonal antibody and a PE-Cy7-conjugated Rat Anti-Mouse IgM Monoclonal Antibody (Novus Biologicals, Catalog # NBP1-42940).