- What Fc blocking reagent does R&D Systems recommend for Flow Cytometry?
- The Fc blocking reagent ideally should be IgG from the same species as the cells which are being stained.
Flow Cytometry Controls
- Are your fluorescently labeled antibodies beginning with catalog # FAB just the Fab fragments?
- FAB is our naming convention. This does not mean that these antibodies are just the Fab fragments. Unless otherwise indicated on the product datasheet, they are whole, IgG antibodies.
- At what concentration should the isotype control antibody be used in a flow cytometry experiment?
- Ideally the concentration of the isotype control antibody should match the concentration of the analyte specific antibody. One could also titrate both the isotype control and the analyte specific antibodies and choose the concentration for each that gives the best signal to noise ratio.
- What Fc blocking reagent does R&D Systems recommend for Flow Cytometry?
- The Fc blocking reagent ideally should be IgG from the same species as the cells which are being stained.