Human CD20 (Research Grade Rituximab Biosimilar) Biotinylated Antibody

Catalog #: FAB9575B Datasheet / COA / SDS

Recombinant Monoclonal Antibody For Research Use Only

Catalog #	Availability	Size / Price	Qty
FAB9575B-100

Detection of MS4A1/CD20 in HEK293 Human cell line Transfected with Human MS4A1/CD20 and eGFP by Flow Cytometry.

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Human CD20 (Research Grade Rituximab Biosimilar) Biotinylated Antibody Summary

Species Reactivity

Human

Specificity

Detects Human CD20 based on Rituximab therapeutic antibody. This non-therapeutic antibody uses the same variable region sequence as the therapeutic antibody Rituximab. This product is for research use only.

Source

Recombinant Monoclonal Human IgG₁ Clone # Hu2

Purification

Protein A or G purified from cell culture supernatant

Immunogen

Human CD20

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Biotin

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Immunocytochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of MS4A1/CD20 in HEK293 Human cell line Transfected with Human MS4A1/CD20 and eGFP by Flow Cytometry. HEK293 Human cell line transfected with (A) Human MS4A1/CD20 or (B) irrelevant transfectants and eGFP was stained with Human Anti-Human MS4A1/CD20 (Rituximab Biosimilar) Biotinylated Monoclonal Antibody (Catalog # FAB9575B) followed by APC-conjugated Streptavidin (Catalog # F0050). View our protocol for Staining Membrane-associated Proteins.

Immunocytochemistry

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CD20 in Daudi Human Cell Line. CD20 was detected in unfixed live Daudi human Burkitt's lymphoma cell line using Human Anti-Human CD20 (Research Grade Rituximab Biosimilar) Biotinylated Monoclonal Antibody (Catalog # FAB9575B) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI (blue). Specific staining was localized to plasma membrane. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: CD20

Rituximab is a biosimilar directed against the extracellular domain of CD20. The biotinylated antibody can be used in flow cytometry or various assay format to measure CD20 expression and can be detected by conjugated streptavidin.

Long Name

Cluster of Differentiation 20

Entrez Gene IDs

931 (Human); 12482 (Mouse); 25316 (Rat); 485430 (Canine); 102118491 (Cynomolgus Monkey)

Alternate Names

B1; B-lymphocyte antigen CD20; B-lymphocyte cell-surface antigen B1; B-lymphocyte surface antigen B1; Bp35; Bp35MGC3969; CD20 antigen; CD20 receptor; CD20; CD20S7; CVID5; LEU-16; Leukocyte surface antigen Leu-16; Ly-44; Membrane-spanning 4-domains subfamily A member 1; membrane-spanning 4-domains, subfamily A, member 1; MS4A1; MS4A2; S7