Human CD31/PECAM-1 Antibody Summary
Extracellular domain
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
Detection of Human CD31/PECAM‑1 by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human CD31/PECAM-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF806) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for CD31/PECAM-1 at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
CD31/PECAM‑1 in HUVECs. CD31/PECAM-1 was detected in immersion fixed HUVEC human umbilical vein endothelial cells using 10 µg/mL Sheep Anti-Human CD31/PECAM-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF806) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Detection of Human CD31/PECAM‑1 by Simple WesternTM. Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line and HepG2 human hepatocellular carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for CD31/PECAM-1 at approximately 173-186 kDa (as indicated) using 10 µg/mL of Sheep Anti-Human CD31/PECAM-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF806) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
CD31/PECAM‑1 Specificity is Shown by Immunocytochemistry in Knockout Cell Line. CD31/PECAM‑1 was detected in immersion fixed THP‑1 human acute monocytic leukemia cell line but is not detected in CD31/PECAM‑1 knockout (KO) THP‑1 human cell line using Sheep Anti-Human CD31/PECAM‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF806) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; NL010) and counterstained with DAPI (blue). Specific staining was localized to cell membrane. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Detection of CD31/PECAM‑1 in human liver. CD31/PECAM‑1 was detected in immersion fixed paraffin-embedded sections of human liver using Sheep Anti-Human CD31/PECAM‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF806) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to endothelial cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Detection of CD31/PECAM‑1 in human liver. CD31/PECAM‑1 was detected in immersion fixed paraffin-embedded sections of human liver using Sheep Anti-Human CD31/PECAM‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF806) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to endothelial cells and sinusoids. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CD31/PECAM-1
The CD31 adhesion molecule, also known as PECAM-1, is expressed in large amounts on endothelial cells at intercellular junctions and on T cell subsets, and to a lesser extent on platelets and most other leukocytes such as monocytes and neutrophils. CD31 binds to itself homotypically, and also to the leukocyte integrin alpha v beta 3 heterotypically. CD31 is required for the transendothelial migration of leukocytes through intercellular junctions of vascular endothelial cells. CD31 has been found in human plasma, and the presence of this circulating isoform is suggested to modulate the transendothelial migration of leukocytes.
Product Datasheets
Citations for Human CD31/PECAM-1 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
36
Citations: Showing 1 - 10
Filter your results:
Filter by:
-
Multisensory gamma stimulation promotes glymphatic clearance of amyloid
Authors: Murdock, MH;Yang, CY;Sun, N;Pao, PC;Blanco-Duque, C;Kahn, MC;Kim, T;Lavoie, NS;Victor, MB;Islam, MR;Galiana, F;Leary, N;Wang, S;Bubnys, A;Ma, E;Akay, LA;Sneve, M;Qian, Y;Lai, C;McCarthy, MM;Kopell, N;Kellis, M;Piatkevich, KD;Boyden, ES;Tsai, LH;
Nature
Species: Human hepegivirus
Sample Types: Organoid
Applications: Immunohistochemistry -
Human iPSC-derived Committed Cardiac Progenitors Generate Cardiac Tissue Grafts in a Swine Ischemic Cardiomyopathy Model without Triggering Ventricular Arrhythmias
Authors: Raval, AN;Schmuck, EG;Roy, S;Saito, Y;Zhou, T;Conklin, J;Hacker, TA;Koonce, C;Boyer, M;Stack, K;Hebron, E;Nagle, SK;Hsieh, PCH;Kamp, TJ;
bioRxiv : the preprint server for biology
Species: Human hepegivirus
Sample Types: Whole Cells
Applications: Immunocytochemistry -
Multi-omics profiling visualizes dynamics of cardiac development and functions
Authors: Y Gu, Y Zhou, S Ju, X Liu, Z Zhang, J Guo, J Gao, J Zang, H Sun, Q Chen, J Wang, J Xu, Y Xu, Y Chen, Y Guo, J Dai, H Ma, C Wang, G Jin, C Li, Y Xia, H Shen, Y Yang, X Guo, Z Hu
Cell Reports, 2022-12-27;41(13):111891.
Species: Mouse
Sample Types: Whole Cells
-
Heightened apoptotic priming of vascular cells across tissues and life span predisposes them to cancer therapy-induced toxicities
Authors: JKE Spetz, MHC Florido, CS Fraser, X Qin, J Choiniere, SJ Yu, R Singh, M Friesen, LL Rubin, JE Salem, JJ Moslehi, KA Sarosiek
Science Advances, 2022-11-09;8(45):eabn6579.
Species: Human, Mouse
Sample Types: Whole Cells, Whole Tissue
Applications: ICC, IHC -
A Personalized Glomerulus Chip Engineered from Stem Cell-Derived Epithelium and Vascular Endothelium
Authors: Y Roye, R Bhattachar, X Mou, Y Zhou, MA Burt, S Musah
Micromachines, 2021-08-16;12(8):.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Key molecular alterations in endothelial cells in human glioblastoma uncovered through single-cell RNA sequencing
Authors: Y Xie, L He, R Lugano, Y Zhang, H Cao, Q He, M Chao, B Liu, Q Cao, J Wang, Y Jiao, Y Hu, L Han, Y Zhang, H Huang, L Uhrbom, C Betsholtz, L Wang, A Dimberg, L Zhang
JCI Insight, 2021-08-09;0(0):.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
SARS-CoV-2 Infects Syncytiotrophoblast and Activates Inflammatory Responses in the Placenta
Authors: LB Argueta, LA Lacko, Y Bram, T Tada, L Carrau, T Zhang, S Uhl, BC Lubor, V Chandar, C Gil, W Zhang, B Dodson, J Bastiaans, M Prabhu, CM Salvatore, YJ Yang, RN Baergen, BR tenOever, NR Landau, S Chen, RE Schwartz, H Stuhlmann
bioRxiv : the preprint server for biology, 2021-06-17;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Mechanisms of angiogenic incompetence in Hutchinson-Gilford progeria via downregulation of endothelial NOS
Authors: YG Gete, LW Koblan, X Mao, M Trappio, B Mahadik, JP Fisher, DR Liu, K Cao
Aging Cell, 2021-06-04;0(0):e13388.
Species: Human
Sample Types: Cell Lysates, Whole Cells
Applications: IHC, Western Blot -
3D iPSC modeling of the retinal pigment epithelium-choriocapillaris complex identifies factors involved in the pathology of macular degeneration
Authors: KV Manian, CA Galloway, S Dalvi, AA Emanuel, JA Mereness, W Black, L Winschel, C Soto, Y Li, Y Song, W DeMaria, A Kumar, I Slukvin, MP Schwartz, WL Murphy, B Anand-Apte, M Chung, DSW Benoit, R Singh
Cell Stem Cell, 2021-03-29;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Generation, functional analysis and applications of isogenic three-dimensional self-aggregating cardiac microtissues from human pluripotent stem cells
Authors: G Campostrin, V Meraviglia, E Giacomelli, RWJ van Helden, L Yiangou, RP Davis, M Bellin, VV Orlova, CL Mummery
Nature Protocols, 2021-03-26;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry, ICC -
Multi-layered Spatial Transcriptomics Identify Secretory Factors Promoting Human Hematopoietic Stem Cell Development
Authors: EI Crosse, S Gordon-Key, S Rybtsov, A Binagui-Ca, H Felchle, NC Nnadi, K Kirschner, T Chandra, S Tamagno, DJ Webb, F Rossi, RA Anderson, A Medvinsky
Cell Stem Cell, 2020-09-17;0(0):.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
Abnormal Microvascular Architecture, Fibrosis, and Pericyte Characteristics in the Calf Muscle of Peripheral Artery Disease Patients with Claudication and Critical Limb Ischemia
Authors: CJ Mietus, TJ Lackner, PS Karvelis, GT Willcockso, CM Shields, NG Lambert, P Koutakis, MA Fuglestad, H Hernandez, GR Haynatzki, JKS Kim, HK DeSpiegela, II Pipinos, GP Casale
J Clin Med, 2020-08-08;9(8):.
Species: Bovine, Human
Sample Types: Whole Cells, Whole Tissue
Applications: ICC, IHC -
A Human Pluripotent Stem Cell-based Platform to Study SARS-CoV-2 Tropism and Model Virus Infection in Human Cells and Organoids
Authors: L Yang, Y Han, BE Nilsson-Pa, V Gupta, P Wang, X Duan, X Tang, J Zhu, Z Zhao, F Jaffré, T Zhang, TW Kim, O Harschnitz, D Redmond, S Houghton, C Liu, A Naji, G Ciceri, S Guttikonda, Y Bram, DT Nguyen, M Cioffi, V Chandar, DA Hoagland, Y Huang, J Xiang, H Wang, D Lyden, A Borczuk, HJ Chen, L Studer, FC Pan, DD Ho, BR tenOever, T Evans, RE Schwartz, S Chen
Cell Stem Cell, 2020-06-19;27(1):125-136.e7.
Species: Human
Sample Types: Organoids, Whole Cells
Applications: ICC, IHC -
Conditioned medium produced by fibroblasts cultured in low oxygen pressure allows the formation of highly structured capillary-like networks in fibrin gels
Authors: C Caneparo, C Baratange, S Chabaud, S Bolduc
Sci Rep, 2020-06-09;10(1):9291.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Reconstruction of the human blood-brain barrier in vitro reveals a pathogenic mechanism of APOE4 in pericytes
Authors: JW Blanchard, M Bula, J Davila-Vel, LA Akay, L Zhu, A Frank, MB Victor, JM Bonner, H Mathys, YT Lin, T Ko, DA Bennett, HP Cam, M Kellis, LH Tsai
Nat. Med., 2020-06-08;26(6):952-963.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Human-iPSC-Derived Cardiac Stromal Cells Enhance Maturation in 3D Cardiac Microtissues and Reveal Non-cardiomyocyte Contributions to Heart Disease
Authors: E Giacomelli, V Meraviglia, G Campostrin, A Cochrane, X Cao, RWJ van Helden, A Krotenberg, M Mircea, S Kostidis, RP Davis, BJ van Meer, CR Jost, AJ Koster, H Mei, DG Míguez, AA Mulder, M Ledesma-Te, G Pompilio, L Sala, DCF Salvatori, RC Slieker, E Sommariva, AAF de Vries, M Giera, S Semrau, LGJ Tertoolen, VV Orlova, M Bellin, CL Mummery
Cell Stem Cell, 2020-05-26;26(6):862-879.e11.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
A Human Pluripotent Stem Cell-based Platform to Study SARS-CoV-2 Tropism and Model Virus Infection in Human Cells and Organoids
Authors: L Yang, Y Han, BE Nilsson-Pa, V Gupta, P Wang, X Duan, X Tang, J Zhu, Z Zhao, F Jaffré, T Zhang, TW Kim, O Harschnitz, D Redmond, S Houghton, C Liu, A Naji, G Ciceri, S Guttikonda, Y Bram, DT Nguyen, M Cioffi, V Chandar, DA Hoagland, Y Huang, J Xiang, H Wang, D Lyden, A Borczuk, HJ Chen, L Studer, FC Pan, DD Ho, BR tenOever, T Evans, RE Schwartz, S Chen
Cell Stem Cell, 2020;27(1):125-136.e7.
Species: Human
Sample Types: Organoids
Applications: IHC -
Mineral deposition and vascular invasion of hydroxyapatite reinforced collagen scaffolds seeded with human adipose-derived stem cells
Authors: HE Weiss-Bilk, MJ Meagher, JA Gargac, GL Niebur, RK Roeder, DR Wagner
Biomater Res, 2019-10-17;23(0):15.
Species: Human
Sample Types: Whole Cells
Applications: Flourescense Microscopy -
Maternal Western-style diet affects offspring islet composition and function in a non-human primate model of maternal over-nutrition
Authors: JM Elsakr, JC Dunn, K Tennant, SK Zhao, K Kroeten, RC Pasek, DL Takahashi, TA Dean, DR Velez Edwa, CE McCurdy, KM Aagaard, AC Powers, JE Friedman, P Kievit, M Gannon
Mol Metab, 2019-04-12;0(0):.
Species: Primate - Macaca fuscata (Japanese Macaque)
Sample Types: Whole Tissue
Applications: IHC-P -
Tissue-engineered 3D melanoma model with blood and lymphatic capillaries for drug development
Authors: J Bourland, J Fradette, FA Auger
Sci Rep, 2018-09-04;8(1):13191.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
Circulating Vascular Basement Membrane Fragments are Associated with the Diameter of the Abdominal Aorta and Their Expression Pattern is Altered in AAA Tissue
Authors: M Holsti, A Wanhainen, C Lundin, M Björck, G Tegler, J Svensson, M Sund
Eur J Vasc Endovasc Surg, 2018-04-12;0(0):.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-Fr -
Leucine-Rich ?-2-Glycoprotein-1 (LRG-1) Expression in Retinoblastoma
Authors: R Amer, L Tiosano, J Pe'er
Invest. Ophthalmol. Vis. Sci., 2018-02-01;59(2):685-692.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
Tissue-engineered 3D human lymphatic microvascular network for in vitro studies of lymphangiogenesis
Authors: L Gibot, T Galbraith, J Bourland, A Rogic, M Skobe, FA Auger
Nat Protoc, 2017-04-27;12(5):1077-1088.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
Laminin-guided highly efficient endothelial commitment from human pluripotent stem cells
Sci Rep, 2016-11-02;6(0):35680.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Aberrant bispecific antibody pharmacokinetics linked to liver sinusoidal endothelium clearance mechanism in cynomolgus monkeys
Authors: A Datta-Mann, JE Croy, L Schirtzing, S Torgerson, M Breyer, VJ Wroblewski
MAbs, 2016-07-01;8(5):969-82.
Species: Primate
Sample Types: Whole Cells
Applications: ICC -
TGF-beta1-induced EMT promotes targeted migration of breast cancer cells through the lymphatic system by the activation of CCR7/CCL21-mediated chemotaxis.
Authors: Pang M, Georgoudaki A, Lambut L, Johansson J, Tabor V, Hagikura K, Jin Y, Jansson M, Alexander J, Nelson C, Jakobsson L, Betsholtz C, Sund M, Karlsson M, Fuxe J
Oncogene, 2015-05-11;35(6):748-60.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
A BMP7 Variant Inhibits Tumor Angiogenesis In Vitro and In Vivo through Direct Modulation of Endothelial Cell Biology.
Authors: Tate C, Mc Entire J, Pallini R, Vakana E, Wyss L, Blosser W, Ricci-Vitiani L, D'Alessandris Q, Morgante L, Giannetti S, Larocca L, Todaro M, Benfante A, Colorito M, Stassi G, De Maria R, Rowlinson S, Stancato L
PLoS ONE, 2015-04-28;10(4):e0125697.
Species: Human
Sample Types: Whole Cells
Applications: IHC-Fr -
IGFBP-4 and -5 are expressed in first-trimester villi and differentially regulate the migration of HTR-8/SVneo cells.
Authors: Crosley, Erin J, Dunk, Caroline, Beristain, Alexande, Christians, Julian K
Reprod Biol Endocrinol, 2014-12-04;12(0):123.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P -
Consumption of a Western-style diet during pregnancy impairs offspring islet vascularization in a Japanese macaque model.
Authors: Pound L, Comstock S, Grove K
Am J Physiol Endocrinol Metab, 2014-05-20;307(1):E115-23.
Species: Primate - Macaca mulatta (Rhesus Macaque)
Sample Types: Whole Tissue
Applications: IHC-P -
A method to assess target gene involvement in angiogenesis in vitro and in vivo using lentiviral vectors expressing shRNA.
Authors: Blosser, Wayne, Vakana, Eliza, Wyss, Lisa V, Swearingen, Michelle, Stewart, Julie, Stancato, Louis, Tate, Courtney
PLoS ONE, 2014-04-23;9(4):e96036.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
miR-27a regulates endothelial differentiation of breast cancer stem like cells.
Authors: Tang, W, Yu, F, Yao, H, Cui, X, Jiao, Y, Lin, L, Chen, J, Yin, D, Song, E, Liu, Q
Oncogene, 2013-06-10;33(20):2629-38.
Species: Human
Sample Types: Cell Lysates, Whole Cells, Whole Tissue
Applications: ICC, IHC-Fr, Western Blot -
Type IV collagen stimulates pancreatic cancer cell proliferation, migration, and inhibits apoptosis through an autocrine loop.
Authors: Ohlund D, Franklin O, Lundberg E, Lundin C, Sund M
BMC Cancer, 2013-03-26;13(0):154.
Species: Human
Sample Types: Whole Cells, Whole Tissue
Applications: ICC, IHC-Fr -
Maternal high-fat diet impacts endothelial function in nonhuman primate offspring.
Authors: Fan, L, Lindsley, S R, Comstock, S M, Takahashi, D L, Evans, A E, He, G-W, Thornburg, K L, Grove, K L
Int J Obes (Lond), 2012-03-27;37(2):254-62.
Species: Primate - Macaca fuscata (Japanese Macaque)
Sample Types: Whole Tissue
Applications: IHC-P -
Proteomic analysis reveals presence of platelet microparticles in endothelial progenitor cell cultures.
Authors: Prokopi M, Pula G, Mayr U, Devue C, Gallagher J, Xiao Q, Boulanger CM, Westwood N, Urbich C, Willeit J, Steiner M, Breuss J, Xu Q, Kiechl S, Mayr M
Blood, 2009-04-15;114(3):723-32.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Signaling hierarchy regulating human endothelial cell development.
Authors: Kelly MA, Hirschi KK
Arterioscler. Thromb. Vasc. Biol., 2009-02-12;29(5):718-24.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Recapitulation of pancreatic neuroendocrine tumors in human multiple endocrine neoplasia type I syndrome via Pdx1-directed inactivation of Men1.
Authors: Shen HC, He M, Powell A, Adem A, Lorang D, Heller C, Grover AC, Ylaya K, Hewitt SM, Marx SJ, Spiegel AM, Libutti SK
Cancer Res., 2009-02-10;69(5):1858-66.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr
FAQs
No product specific FAQs exist for this product, however you may
View all Antibody FAQsReviews for Human CD31/PECAM-1 Antibody
Average Rating: 4.5 (Based on 2 Reviews)
Have you used Human CD31/PECAM-1 Antibody?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
Filter by: