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Human MIF Antibody

Catalog #: MAB289
17 Citations 6 Reviews Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB289-SP
MAB289-100
MAB289-500
Detection of Human MIF by Western Blot.
2 Images
Product Details
Citations (17)
FAQs
Supplemental Products
Reviews (6)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human MIF Antibody Summary

Species Reactivity
Human
Specificity
Detects human MIF in Western blots. In Western blots, this antibody shows approximately 25% cross-reactivity with recombinant mouse MIF.
Source
Monoclonal Mouse IgG1 Clone # 12302
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human MIF
Pro2-Ala115
Accession # P14174
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Simple Western
10 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human MIF antibody by Western Blot. View Larger

Detection of Human MIF by Western Blot. Western blot shows lysate of K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human MIF Monoclonal Antibody (Catalog # MAB289) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for MIF at approximately 12 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western Detection of Humean MIF antibody by Simple Western<sup>TM</sup>. View Larger

Detection of Humean MIF by Simple WesternTM. Simple Western lane view shows lysates of THP‑1 human acute monocytic leukemia cell line and U937 human histiocytic lymphoma cell line, loaded at 0.2 mg/mL. A specific band was detected for MIF at approximately 12 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human MIF Monoclonal Antibody (Catalog # MAB289). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MIF

MIF (or macrophage migration inhibitory factor) was the first lymphokine/cytokine to be recognized in the pregenomics era (1, 2). Regardless, it is one of the least understood of all inflammatory mediators (1, 3). Human MIF is a 12.5 kDa, 115 amino acid (aa) nonglycosylated polypeptide that is synthesized without a signal sequence (4-7). Secretion occurs nonclassically via an ABCA1 transporter (8). The initiating Met is removed, leaving Pro as the first amino acid. The molecule consists of two alpha -helices and six beta -strands, four of which form a beta -sheet. The two remaining beta -strands interact with other MIF molecules, creating a trimer (2, 9, 10). Structure-function studies suggest MIF is bifunctional with segregated topology. The N- and C-termini mediate enzyme activity (in theory). Phenylpyruvate tautomerase activity (enol-to-keto) has been demonstrated and is dependent upon Pro at position #1 (11). Amino acids 50-65 have also been suggested to contain thiol-protein oxidoreductase activity (12). MIF has proinflammatory cytokine activity centered around aa’s 49-65. On fibroblasts, MIF induces, IL-1, IL-8, and MMP expression; on macrophages, MIF stimulates NO production and TNF-alpha release following IFN-gamma activation (13, 14). MIF apparently acts through CD74 and CD44, likely in some form of trimeric interaction (15, 16). Human MIF is active on mouse cells (14). Human MIF is 90%, 94%, 95%, and 90% aa identical to mouse, bovine, porcine, and rat MIF, respectively.

References
  1. Norand, E.F. and M. Leech (2005) Front. Biosci. 10:12.
  2. Donn, R.P. and D.W. Ray (2004) J. Endocrinol. 182:1.
  3. Calandra, T. and T. Roger (2003) Nat. Rev. Immunol. 3:791.
  4. Kozak, C.A. et al. (1995) Genomics 27:405.
  5. Weiser, W.Y. et al. (1989) Proc. Natl. Acad. Sci. USA 86:7522.
  6. Paralkar, V. and G. Wistow (1994) Genomics 19:48.
  7. Wistow, G.J. et al. (1993) Proc. Natl. Acad. Sci. USA 90:1272.
  8. Flieger, O. et al. (2003) FEBS Lett. 551:78.
  9. Philo, J.S. et al. (2004) Biophys. Chem. 108:77.
  10. Sun, H-W. et al. (1996) Protein Eng. 9:631.
  11. Stamps, S.L. et. al. (2000) Biochemistry 39:9671.
  12. Nguyen, M.T. et al. (2003) J. Biol. Chem. 278:33654.
  13. Sato, A. et al. (2003) Dev. Comp. Immunol. 27:401.
  14. Bernhagen, J. et al. (1994) Biochemistry 33:14144.
  15. Leng, L. et al. (2003) J. Exp. Med. 197:1467.
  16. Meyer-Siegler, K.L. and P.L. Vera (2005) J. Urol. 173:615.
Long Name
Macrophage Migration Inhibitory Factor
Entrez Gene IDs
4282 (Human); 17319 (Mouse)
Alternate Names
EC 5.3.2.1; EC 5.3.3.12; GIFmacrophage migration inhibitory factor; GLIF; Glycosylation-inhibiting factor; L-dopachrome isomerase; L-dopachrome tautomerase; macrophage migration inhibitory factor (glycosylation-inhibiting factor); MIF; MMIF; Phenylpyruvate tautomerase

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Citations for Human MIF Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

17 Citations: Showing 1 - 10
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  1. Proteomics of Primary Uveal Melanoma: Insights into Metastasis and Protein Biomarkers
    Authors: GF Jang, JS Crabb, B Hu, B Willard, H Kalirai, AD Singh, SE Coupland, JW Crabb
    Cancers, 2021-07-14;13(14):.
    Species: Human
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  2. Migration Inhibitory Factor in Conditioned Medium from Human Umbilical Cord Blood-Derived Mesenchymal Stromal Cells Stimulates Hair Growth
    Authors: HA Oh, J Kwak, BJ Kim, HJ Jin, WS Park, SJ Choi, W Oh, S Um
    Cells, 2020-05-28;9(6):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  3. Ibudilast sensitizes glioblastoma to temozolomide by targeting Macrophage Migration Inhibitory Factor (MIF)
    Authors: W Ha, H Sevim-Nalk, AM Zaman, K Matsuda, M Khasraw, AK Nowak, L Chung, RC Baxter, KL McDonald
    Sci Rep, 2019-02-27;9(1):2905.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  4. Association of Macrophage Migration Inhibitory Factor Polymorphisms with Total Plasma IgE Levels in Patients with Atopic Dermatitis in Korea
    Authors: JS Kim, J Choi, HJ Hahn, YB Lee, DS Yu, JW Kim
    PLoS ONE, 2016-09-06;11(9):e0162477.
    Species: Human
    Sample Types: Plasma
    Applications: ELISA Development (Capture)
  5. Macrophage Migration Inhibitory Factor in Acute Adipose Tissue Inflammation.
    Authors: Kim B, Rongisch R, Hager S, Grieb G, Nourbakhsh M, Rennekampff H, Bucala R, Bernhagen J, Pallua N
    PLoS ONE, 2015-09-08;10(9):e0137366.
    Species: Human
    Sample Types: Tissue Homogenates, Whole Tissue
    Applications: ELISA Development (Capture), IHC-P
  6. iTRAQ Quantitative Proteomic Comparison of Metastatic and Non-Metastatic Uveal Melanoma Tumors.
    Authors: Crabb J, Hu B, Crabb J, Triozzi P, Saunthararajah Y, Tubbs R, Singh A
    PLoS ONE, 2015-08-25;10(8):e0135543.
    Species: Human
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  7. Macrophage migration inhibitory factor engages PI3K/Akt signalling and is a prognostic factor in metastatic melanoma.
    Authors: Oliveira, Camila S, de Bock, Charles, Molloy, Timothy, Sadeqzadeh, Elham, Geng, Xin Yan, Hersey, Peter, Zhang, Xu Dong, Thorne, Rick F
    BMC Cancer, 2014-08-29;14(0):630.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  8. A multiplex immunoassay for human adipokine profiling.
    Authors: Schipper HS, De Jager W, van Dijk ME, Meerding J, Zelissen PM, Adan RA, Prakken BJ, Kalkhoven E
    Clin. Chem., 2010-06-08;56(0):1320.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Luminex Development
  9. Macrophage migration inhibitory factor stimulates angiogenic factor expression and correlates with differentiation and lymph node status in patients with esophageal squamous cell carcinoma.
    Authors: Ren Y, Law S, Huang X, Lee PY, Bacher M, Srivastava G, Wong J
    Ann. Surg., 2005-07-01;242(1):55-63.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  10. Intracellular interaction of myosin light chain kinase with macrophage migration inhibition factor (MIF) in endothelium.
    Authors: Wadgaonkar R, Dudek SM, Zaiman AL, Linz-McGillem L, Verin AD, Nurmukhambetova S, Romer LH, Garcia JG
    J. Cell. Biochem., 2005-07-01;95(4):849-58.
    Species: Bovine, Human
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation
  11. Up-regulation of macrophage migration inhibitory factor in infants with acute neonatal necrotizing enterocolitis.
    Authors: Ren Y, Lin CL, Li Z, Chen XY, Huang X, Lui V, Nicholls J, Lan HY, Tam PK
    Histopathology, 2005-06-01;46(6):659-67.
    Species: Human
    Sample Types: Serum
    Applications: Neutralization
  12. Elevated macrophage migration inhibitory factor (MIF) levels in the urine of patients with focal glomerular sclerosis.
    Authors: Matsumoto K, Maruyama N, Maruyama T, Ohnishi Y, Nonaka S, Inoshita A, Ito K, Kitajima S, Abe M, Satomura A, Fujita T
    Clin. Exp. Immunol., 2005-02-01;139(2):338-47.
    Species: Human
    Sample Types: Urine, Whole Tissue
    Applications: ELISA Development, IHC
  13. Regulatory role of C5a on macrophage migration inhibitory factor release from neutrophils.
    Authors: Riedemann NC, Guo RF, Gao H, Sun L, Hoesel M, Hollmann TJ, Wetsel RA, Zetoune FS, Ward PA
    J. Immunol., 2004-07-15;173(2):1355-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  14. High concentrations of circulating macrophage migration inhibitory factor in patients with severe blunt trauma: Is serum macrophage migration inhibitory factor concentration a valuable prognostic factor?
    Authors: Chuang CC, Hung CJ, Tsai MC, Chuang YC
    Crit. Care Med., 2004-03-01;32(3):734-9.
    Species: Human
    Sample Types: Serum
    Applications: ELISA Development
  15. Estrogen modulates cutaneous wound healing by downregulating macrophage migration inhibitory factor.
    Authors: Ashcroft GS, Mills SJ, Lei K, Gibbons L, Jeong MJ, Taniguchi M, Burow M, Horan MA, Wahl SM, Nakayama T
    J. Clin. Invest., 2003-05-01;111(9):1309-18.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  16. Urinary levels of macrophage migration inhibitory factor in patients with IgA nephropathy.
    Authors: Matsumoto K, 2019, Kanmatsuse K
    7587, 2002-10-01;92(2):309-15.
    Species: Human
    Sample Types: Urine
    Applications: ELISA Development
  17. Human V gamma 2V delta 2 T cells augment migration-inhibitory factor secretion and counteract the inhibitory effect of glucocorticoids on IL-1 beta and TNF-alpha production.
    Authors: Wang L, Das H, Kamath A, Li L, Bukowski JF
    J. Immunol., 2002-05-15;168(10):4889-96.
    Species: Human
    Sample Types: Cell Culture Supernates

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Reviews for Human MIF Antibody

Average Rating: 4.2 (Based on 6 Reviews)

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Human MIF Antibody
By Anonymous on 01/14/2021
Application: Microarrays Sample Tested: EDTA Plasma Species: Human
Other Human MIF Antibody MAB289
Human MIF Antibody
By Anonymous on 07/19/2019
Application: ELISA Sample Tested: Serum and Plasma Species: Human
ELISA Human MIF Antibody MAB289
Human MIF Antibody
By Carly Garrison on 11/20/2018
Application: MiAr Sample Tested: EDTA Plasma Species: Human
Human MIF Antibody
By Anonymous on 11/14/2018
Application: Microarrays Sample Tested: EDTA Plasma Species: Human
Human MIF Antibody
By Anonymous on 11/08/2018
Application: ELISA Sample Tested: Serum and Plasma Species: Human
Human MIF Antibody
By Anonymous on 11/07/2017
Application: ELISA Sample Tested: Serum and Plasma Species: Human

This antibody was used as a matched pair with AF289 to build a sandwich ELISA to measure MIF in human serum and plasma. The antibody worked well.

ELISA Human MIF Antibody MAB289