Human/Mouse AMIGO2 Antibody

Catalog # Availability Size / Price Qty
MAB2374
MAB2374-SP
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Human/Mouse AMIGO2 Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects mouse AMIGO2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, 100% cross-reactivity with recombinant human (rh) AMIGO2 and no cross-reactivity with rhAMIGO1 or recombinant mouse AMIGO3 is observed.
Source
Monoclonal Rat IgG2A Clone # 290728
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse AMIGO2
Met39-Ala391
Accession # Q80ZD9
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Purity
Protein A or G purified from hybridoma culture supernatant

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human and mouse AMIGO2

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: AMIGO2

AMIGO2 is a member of the AMIGO family of type I transmembrane proteins that contain 6 leucine-rich repeats (LRRs) and one Ig domain in the extracellular region. AMIGO2 is expressed primarily in cerebellum, retina, liver, and lung. AMIGO family proteins are cell adhesion molecules that exhibit homophilic and heterophilic binding properties.

Long Name
Amphoterin-induced Gene and ORF 2
Entrez Gene IDs
347902 (Human); 105827 (Mouse); 102124161 (Cynomolgus Monkey)
Alternate Names
adhesion molecule with Ig-like domain 2; ALI1amphoterin induced gene 2; alivin 1; Alivin-1; AMIGO2; AMIGO-2; DEGAamphoterin-induced protein 2; differentially expressed in gastric adenocarcinoma; Differentially expressed in gastric adenocarcinomas; transmembrane protein AMIGO2

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