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Human/Mouse Oct-3/4 Antibody

Catalog #: MAB1759
38 Citations 1 Review Datasheet / COA / SDS
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MAB1759
MAB1759-SP
Detection of Human Oct-3/4 by Western Blot.
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Product Details
Citations (38)
FAQs
Supplemental Products
Reviews (1)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse Oct-3/4 Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human Oct-3/4 in Western blots and detects mouse Oct-3/4 in flow cytometry.
Source
Monoclonal Rat IgG2B Clone # 240408
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human Oct‑3/4
Met1-Asn265 (Met262Leu)
Accession # Q01860
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
8-25 µg/mL
See below
Intracellular Staining by Flow Cytometry
2.5 µg/106 cells
BG01V human embryonic stem cells and D3 mouse embryonic stem cell line fixed with paraformaldehyde and permeabilized with saponin

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human Oct-3/4 antibody by Western Blot. View Larger

Detection of Human Oct-3/4 by Western Blot. Western blot shows lysates of BG01V human embryonic stem cells and NTera-2 human testicular embryonic carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Rat Anti-Human/Mouse Oct-3/4 Monoclonal Antibody (Catalog # MAB1759) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Oct-3/4 at approximately 46 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry Oct-3/4 antibody in BG01V Human Embryonic Stem Cells by Immunocytochemistry (ICC). View Larger

Oct‑3/4 in BG01V Human Embryonic Stem Cells. Oct‑3/4 was detected in immersion fixed BG01V human embryonic stem cells using Rat Anti-Human/Mouse Oct‑3/4 Monoclonal Antibody (Catalog # MAB1759) at 10 µg/mL for 3 hours at room temperature. Cells were stained using Rhodamine Red-coupled anti-rat IgG (red) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Oct-3/4

Oct-3/4, also termed Oct-3 or Oct-4, is a POU transcription factor that is expressed in totipotent embryonic stem and germ cells.(2, 3) Oct-3/4 is required to sustain stem cell self-renewal and pluripotency.(4) It is considered a master regulator of pluripotency that controls lineage commitment and is the most widely recognized marker of totipotent embryonic stem cells.

References
  1. Takeda, J. et al. (1992) Nucleic Acids Res. 20:4613.
  2. Scholer, H.R. et al. (1989) EMBO J. 8:2543.
  3. Rosner, M.H. et al. (1990) Nature 345:686.
  4. Niwa, H. et al. (2000) Nat. Genet. 24:372.
Long Name
Octamer-binding Protein 3/4
Entrez Gene IDs
5460 (Human); 18999 (Mouse)
Alternate Names
3-Oct; Oct-3/4; OCT3;Oct-3;OCT4;Oct-4;OCT4;Oct3/4;OTF3;OTF-3;OTF4;POU domain protein;POU domain, class 5, transcription factor 1;POU5F1; Oct4; Otf3g; Otf4; Pou5f1

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Citations for Human/Mouse Oct-3/4 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

38 Citations: Showing 1 - 10
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  1. Generation of a DMD loss-of-function mutant human embryonic stem cell lines by CRISPR base editing
    Authors: Jin, H;Fu, H;Wang, J;Wang, Z;Liu, J;Han, F;Zheng, H;Jiang, Y;
    Stem cell research
    Species: Human hepegivirus
    Sample Types: Transfected Whole Cells
    Applications: Immunocytochemistry
  2. Generation of a PPP1CA knockout human pluripotent stem cell line via CRISPR/Cas9
    Authors: X Wang, J Sun, C Fu, C Liu
    Stem Cell Research, 2023-03-21;69(0):103077.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry, ICC
  3. Establishment of a PPP2CA homozygous knockout human embryonic stem cell line via CRISPR/Cas9 system
    Authors: T Ding, Z Gao, H Liu, Y Li, S Liu, L Jian
    Stem Cell Research, 2023-01-16;67(0):103029.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Generation of a homozygous TAZ knockout hESCs line by CRISPR/Cas9 system
    Authors: M Zhou, P Huang, R Bai, X Liu
    Stem Cell Research, 2022-09-20;64(0):102923.
    Species: Human
    Sample Types: Transfected Whole Cells
    Applications: ICC
  5. The Use of Trichostatin A during Pluripotent Stem Cell Generation Does Not Affect MHC Expression Level
    Authors: S Farahi, S Hosseini, H Ghanbarian, SM Hashemi, M Salehi, S Hosseini
    Stem Cells International, 2022-02-15;2022(0):9346767.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC/IF
  6. Brahma safeguards canalization of cardiac mesoderm differentiation
    Authors: SK Hota, KS Rao, AP Blair, A Khalilimey, KM Hu, R Thomas, K So, V Kameswaran, J Xu, BJ Polacco, RV Desai, N Chatterjee, A Hsu, JM Muncie, AM Blotnick, SAB Winchester, LS Weinberger, R Hüttenhain, IS Kathiriya, NJ Krogan, JJ Saucerman, BG Bruneau
    Nature, 2022-01-26;602(7895):129-134.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  7. LncCDH5-3:3 Regulates Apoptosis, Proliferation, and Aggressiveness in Human Lung Cancer Cells
    Authors: K Kwa?niak, J Czarnik-Kw, K Malysheva, K Pogoda, O Korchynsky, P Rybojad, B Karczmarek, J Tabarkiewi
    Cells, 2022-01-23;11(3):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  8. Generation of a homozygous S100A1 knockout human embryonic stem cell line (WAe009-A-73) by the CRISPR/Cas9 editing system
    Authors: S Zhang, T Guo, Y Song, M Jiang, Y Jiang, T Dong, S Zhang, H Wang, X Hou
    Stem Cell Research, 2021-12-20;59(0):102631.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry, ICC
  9. Establishment of a KCNQ1 homozygous knockout human embryonic stem cell line by episomal vector-based CRISPR/Cas9 system
    Authors: X Liu, S Zhang, Y Chang, F Wu, R Bai
    Stem Cell Research, 2021-07-22;55(0):102467.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry, ICC
  10. Generating dHAND homozygous knockout human embryonic stem cell line (WAe009-A-59) by episomal vector-based CRISPR/Cas9 system
    Authors: W Wang, R Bai, X Song
    Stem Cell Research, 2021-07-20;55(0):102471.
    Species: Human
    Sample Types: Whole Cell
    Applications: Flow Cytometry, ICC
  11. Generation of a TPM1 homozygous knockout embryonic stem cell line by CRISPR/Cas9 editing
    Authors: S Ma, Q Xu, R Bai, T Dong, Z Peng, X Liu
    Stem Cell Research, 2021-07-19;55(0):102470.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  12. Generation of a TLR2 homozygous knockout human embryonic stem cell line WAe001-A-64 using CRISPR/Cas9 editing
    Authors: A Getachew, Z Yang, X Huang, F Wu, YY Liu, C Yan, F Yang, Y Li
    Stem Cell Research, 2021-05-21;54(0):102401.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  13. Generation of a TBX5 homozygous knockout embryonic stem cell line (WAe009-A-45) by CRISPR/Cas9 genome editing
    Authors: T Zhao, R Bai, F Wu, WJ Lu, J Zhang
    Stem Cell Research, 2021-01-06;51(0):102156.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry, ICC
  14. Differentiation of induced pluripotent stem cells into definitive endoderm on Activin A-functionalized gradient surfaces
    Authors: L Andreasson, H Evenbratt, R Mobini, S Simonsson
    J Biotechnol, 2020-11-02;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay, ICC
  15. Generation of a Junctophilin-2 homozygous knockout human embryonic stem cell line (WAe009-A-36) by an episomal vector-based CRISPR/Cas9 system
    Authors: F Wu, X Li, R Bai, Y Li, J Gao, F Lan
    Stem Cell Res, 2020-08-03;48(0):101930.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  16. Generation of an integration-free induced pluripotent stem cell (iPSC) line (ZZUNEUi005-A) from a Wilson's disease patient harboring a homozygous Pro992Leu mutation in ATP7B gene
    Authors: L Wei, J Zhang, D Chen, L Feng, C Wu, R Wang, X Li
    Stem Cell Res, 2020-07-15;47(0):101909.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  17. Generation of an integration-free induced pluripotent stem cell (iPSC) line (ZZUNEUi002-A) from a patient with spinocerebellar ataxia type 3
    Authors: L Wei, J Zhang, D Chen, L Feng, C Wu, R Wang, X Li
    Stem Cell Res, 2020-06-29;47(0):101898.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  18. The self-renewal dental pulp stem cell microtissues challenged by a toxic dental monomer
    Authors: G Kaufman, NM Kiburi, D Skrtic
    Biosci. Rep., 2020-06-26;40(6):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  19. Generation of an integration-free induced pluripotent stem cell (iPSC) line (ZZUNEUi001-A) from a healthy male individual
    Authors: J Zhang, L Wei, R Wang, X Li, Z Liu
    Stem Cell Res, 2020-04-24;45(0):101809.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  20. Generation of an integration-free induced pluripotent stem cell (iPSC) line (ZZUNEUi004-A) from a Wilson's disease patient harboring a homozygous Pro992Leu mutation in ATP7B gene
    Authors: J Zhang, L Wei, D Chen, L Feng, C Wu, R Wang, X Li, H Liu
    Stem Cell Res, 2020-02-20;44(0):101741.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  21. Effect of ectopic high expression of transcription factor OCT4 on the "stemness" characteristics of human bone marrow-derived mesenchymal stromal cells
    Authors: X Guo, Y Tang, P Zhang, S Li, Y Chen, B Qian, H Shen, N Zhao
    Stem Cell Res Ther, 2019-06-03;10(1):160.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  22. Generation of a GDE heterozygous mutation human embryonic stem cell line WAe001-A-14 by CRISPR/Cas9 editing
    Authors: G Xu, D Guo, F Wu, N Abbas, K Lai, F Yuan, K You, Y Liu, Y Zhuang, Y Wu, Y Xu, Y Chen, F Yang, T Pan, YX Li
    Stem Cell Res, 2017-12-13;27(0):38-41.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  23. Generation of a SMO homozygous knockout human embryonic stem cell line WAe001-A-16 by CRISPR/Cas9 editing
    Authors: F Wu, G Gao, T Pan, Z Yang, G Xu, N Abbas, Y Liu, Y Chen, S Tan, K You, X Ke, Y Zhuang, X Lin, F Yang, YX Li
    Stem Cell Res, 2017-12-05;27(0):5-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  24. Generation of an ASGR1 homozygous mutant human embryonic stem cell line WAe001-A-6 using CRISPR/Cas9
    Authors: Y Xu, Y Wu, D Guo, G Gao, K Lai, F Yang, K Wang, H Wu, L Lai, J Li, K Xu, YX Li
    Stem Cell Res, 2017-05-19;22(0):29-32.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  25. HIF1? regulates single differentiated glioma cell dedifferentiation to stem-like cell phenotypes with high tumorigenic potential under hypoxia
    Authors: P Wang, C Lan, S Xiong, X Zhao, Y Shan, R Hu, W Wan, S Yu, B Liao, G Li, J Wang, D Zou, B Chen, H Feng, N Wu
    Oncotarget, 2017-04-25;8(17):28074-28092.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: ICC, Western Blot
  26. Spatial development of gingival fibroblasts and dental pulp cells: Effect of extracellular matrix
    Authors: G Kaufman, D Skrtic
    Tissue Cell, 2017-04-10;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  27. Molecular and cellular features of murine craniofacial and trunk neural crest cells as stem cell-like cells.
    Authors: Hagiwara, Kunie, Obayashi, Takeshi, Sakayori, Nobuyuki, Yamanishi, Emiko, Hayashi, Ryuhei, Osumi, Noriko, Nakazawa, Toru, Nishida, Kohji
    PLoS ONE, 2014-01-20;9(1):e84072.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  28. Suppression of OCT4B enhances sensitivity of lung adenocarcinoma A549 cells to cisplatin via increased apoptosis.
    Authors: Cortes-Dericks, Lourdes, Yazd, Ehsan Fa, Mowla, Seyed J, Schmid, Ralph A, Karoubi, Golnaz
    Anticancer Res, 2013-12-01;33(12):5365-73.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  29. The effect of human and mouse fibroblast feeder cells on cardiac differentiation of human pluripotent stem cells.
    Authors: Pekkanen-Mattila M, Ojala M, Kerkela E, Rajala K, Skottman H, Aalto-Setala K
    Stem Cells Int, 2012-01-19;2012(0):875059.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  30. Generation of HIV-1 resistant and functional macrophages from hematopoietic stem cell-derived induced pluripotent stem cells.
    Authors: Kambal A, Mitchell G, Cary W, Gruenloh W, Jung Y, Kalomoiris S, Nacey C, McGee J, Lindsey M, Fury B, Bauer G, Nolta JA, Anderson JS
    Mol. Ther., 2010-11-30;19(3):584-93.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  31. A defined glycosaminoglycan-binding substratum for human pluripotent stem cells.
    Authors: Klim JR, Li L, Wrighton PJ
    Nat. Methods, 2010-11-14;7(12):989-94.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  32. Innate immune suppression enables frequent transfection with RNA encoding reprogramming proteins.
    Authors: Angel M, Yanik MF
    PLoS ONE, 2010-07-23;5(7):e11756.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  33. Multipotent mesenchymal stem cells from human placenta: critical parameters for isolation and maintenance of stemness after isolation.
    Authors: Semenov O, Koestenbauer S, Riegel M, Zech N, Zimmermann R, Zisch A, Malek A
    Am J Obstet Gynecol, 2009-12-24;202(2):193.e1-193.e1.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  34. Histone deacetylase inhibition elicits an evolutionarily conserved self-renewal program in embryonic stem cells.
    Authors: Ware CB, Wang L, Mecham BH, Shen L, Nelson AM, Bar M, Lamba DA, Dauphin DS, Buckingham B, Askari B, Lim R, Tewari M, Gartler SM, Issa JP, Pavlidis P, Duan Z, Blau CA
    Cell Stem Cell, 2009-04-03;4(4):359-69.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  35. Octamer 4 small interfering RNA results in cancer stem cell-like cell apoptosis.
    Authors: Hu T, Liu S, Breiter DR, Wang F, Tang Y, Sun S
    Cancer Res., 2008-08-15;68(16):6533-40.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  36. Enhancing the reliability and throughput of neurosphere culture on hydrogel microwell arrays.
    Authors: Cordey M, Limacher M, Kobel S, Taylor V, Lutolf MP
    Stem Cells, 2008-07-31;26(10):2586-94.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  37. Nucleofection mediates high-efficiency stable gene knockdown and transgene expression in human embryonic stem cells.
    Authors: Hohenstein KA, Pyle AD, Chern JY, Lock LF, Donovan PJ
    Stem Cells, 2008-03-06;26(6):1436-43.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  38. Differential requirements for hematopoietic commitment between human and rhesus embryonic stem cells.
    Authors: Rajesh</LastName><ForeNam D</Initial, Rajesh D, Chinnasamy N, Mitalipov SM, Wolf DP, Slukvin I, Thomson JA, Shaaban AF
    Stem Cells, 2007-02-01;25(2):490-9.
    Species: Human, Primate - Macaca mulatta (Rhesus Macaque)
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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Human/Mouse Oct-3/4 Antibody
By Anonymous on 02/09/2022
Application: WB Sample Tested: NTera-2 human testicular embryonic carcinoma cell line Species: Human
Western Blot Human/Mouse Oct-3/4 Antibody MAB1759