Human/Mouse/Rat PLC-gamma 1 Antibody

Catalog #: AF3288 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF3288
AF3288-SP

Detection of Human/Mouse/Rat PLC-gamma 1 by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat PLC-gamma 1 Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects human, mouse, and rat PLC-gamma 1 in Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human PLC-gamma 1
Ser1091-Leu1290
Accession # P19174

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Purity

Immunogen affinity purified

Applications

Recommended Concentration

Sample

Western Blot

0.5 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human/Mouse/Rat PLC-gamma 1 by Western Blot. Western blot shows lysates of HEK293 human embryonic kidney cell line, Balb/3T3 mouse embryonic fibroblast cell line, and Nb2-11 rat lymphoma cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse/Rat PLC- gamma1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3288) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for PLC- gamma1 at approximately 155 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PLC-gamma 1

Phospholipase C gamma-1 (PLC-gamma 1) is activated by receptor tyrosine kinases in response to growth factors and hormones. Activated PLC-gamma 1 catalyzes the hydrolysis of phosphatidylinositol 4,5-biphosphate to produce the second messengers inositol 1,4,5-triphosphate (IP₃) and diacylglycerol (DAG). IP₃mobilizes the release of calcium while DAG activates protein kinase C. PLC-gamma 1 plays an important role in regulating cell proliferation and differentiation.

Long Name

Phospholipase C gamma 1

Entrez Gene IDs

5335 (Human); 18803 (Mouse); 25738 (Rat)

Alternate Names

EC 3.1.4.11; gamma 1 (formerly subtype 148); monophosphatidylinositol phosphodiesterase; NCKAP3,1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase gamma-1; phosphatidylinositol phospholipase C; phosphoinositidase C; phosphoinositide phospholipase C; Phosphoinositide phospholipase C-gamma-1; phospholipase C, gamma 1; phospholipase C-148; Phospholipase C-gamma-1; Phospholipase C-II; PLC11-phosphatidylinositol-4,5-bisphosphate phosphodiesterase gamma 1; PLC148; PLC-148; PLCG1; PLCgamma 1; PLC-gamma 1; PLC-gamma-1; PLC-II1-phosphatidyl-D-myo-inositol-4,5-bisphosphate; PPLCA; triphosphoinositide phosphodiesterase