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Human PLZF Antibody

Catalog #: AF2944
19 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF2944
AF2944-SP
Detection of Human PLZF by Western Blot.
2 Images
Product Details
Citations (19)
FAQs
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human PLZF Antibody Summary

Species Reactivity
Human
Specificity
Detects human PLZF in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human PLZF
Met1-Gln254
Accession # Q05516
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated
Purity
Immunogen affinity purified

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Simple Western
10 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human PLZF antibody by Western Blot. View Larger

Detection of Human PLZF by Western Blot. Western blot shows lysates of HUVEC human umbilical vein endothelial cells, HEL 92.1.7 human erythroleukemic cell line, LNCaP human prostate cancer cell line, and 293T human embryonic kidney cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human PLZF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2944) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for PLZF at approximately 85 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western Detection of Human PLZF antibody by Simple Western<sup>TM</sup>. View Larger

Detection of Human PLZF by Simple WesternTM. Simple Western lane view shows lysates of LNCaP human prostate cancer cell line, loaded at 0.2 mg/mL. A specific band was detected for PLZF at approximately 94 kDa (as indicated) using 10 µg/mL of Goat Anti-Human PLZF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2944) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PLZF

Human PLZF is a 74 kDa nuclear protein that belongs to the POK family of transcriptional repressors. It is a 673 amino acid protein that contains an N-terminal BTB domain, followed by an acidic domain, a proline-rich region and a C-terminal zinc-finger domain. PLZF forms homodimers with RAR alpha and LAZ3 with its zinc-finger region. Alternate splice forms exist which are tissue-specific and show a deletion of either the BTB domain, the acidic region, or the proline-rich region. Human PLZF shares 96%, 97%, 96%, and 96% amino acid identity with rat, mouse, canine, and bovine PLZF, respectively.

Long Name
Promyelocytic Leukemia Zinc Finger
Entrez Gene IDs
7704 (Human); 235320 (Mouse)
Alternate Names
PLZF; PLZFzinc finger and BTB domain-containing protein 16; ZBTB16; zinc finger and BTB domain containing 16; zinc finger protein 145 (Kruppel-like, expressed in promyelocytic leukemia); Zinc finger protein 145; Zinc finger protein PLZF; ZNF145; ZNF145Promyelocytic leukemia zinc finger protein

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Citations for Human PLZF Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

19 Citations: Showing 1 - 10
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  1. Inter- and trans-generational impacts of real-world PM2.5 exposure on male-specific primary hypogonadism
    Authors: Wei, X;Zhang, Z;Gu, Y;Zhang, R;Huang, J;Li, F;He, Y;Lu, S;Wu, Y;Zeng, W;Liu, X;Liu, C;Liu, J;Ao, L;Shi, F;Chen, Q;Lin, Y;Du, J;Jin, G;Xia, Y;Ma, H;Zheng, Y;Huo, R;Cao, J;Shen, H;Hu, Z;
    Cell discovery
    Species: Murine polyomavirus strain A3
    Sample Types: Whole Tissue
    Applications: Immunohistochemistry
  2. SRSF2 is required for mRNA splicing during spermatogenesis
    Authors: Lei, WL;Du, Z;Meng, TG;Su, R;Li, YY;Liu, W;Sun, SM;Liu, MY;Hou, Y;Zhang, CH;Gui, Y;Schatten, H;Han, Z;Liu, C;Sun, F;Wang, ZB;Qian, WP;Sun, QY;
    BMC biology
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Immunocytochemistry
  3. Divergent transcriptional regulation of astrocyte reactivity across disorders
    Authors: JE Burda, TM O'Shea, Y Ao, KB Suresh, S Wang, AM Bernstein, A Chandra, S Deverasett, R Kawaguchi, JH Kim, S McCallum, A Rogers, S Wahane, MV Sofroniew
    Nature, 2022-05-25;0(0):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  4. Intraovarian, Isoform-Specific Transcriptional Roles of Progesterone Receptor in Ovulation
    Authors: KM Smith, DT Dinh, LK Akison, M Nicholls, KR Dunning, A Morimoto, JP Lydon, DL Russell, RL Robker
    Cells, 2022-05-05;11(9):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  5. The netrin-1 receptor UNC5C contributes to the homeostasis of undifferentiated spermatogonia in adult mice
    Authors: V Barroca, C Racine, L Pays, P Fouchet, M Coureuil, I Allemand
    Stem Cell Research, 2022-02-25;60(0):102723.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. A proximity biotinylation-based approach to identify protein-E3 ligase interactions induced by PROTACs and molecular glues
    Authors: S Yamanaka, Y Horiuchi, S Matsuoka, K Kido, K Nishino, M Maeno, N Shibata, H Kosako, T Sawasaki
    Nature Communications, 2022-01-10;13(1):183.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  7. Specific deletion of protein phosphatase 6 catalytic subunit in Sertoli cells leads to disruption of spermatogenesis
    Authors: WL Lei, YY Li, TG Meng, Y Ning, SM Sun, CH Zhang, Y Gui, ZB Wang, WP Qian, QY Sun
    Cell Death & Disease, 2021-09-27;12(10):883.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  8. DAZL mediates a broad translational program regulating expansion and differentiation of spermatogonial progenitors
    Authors: MM Mikedis, Y Fan, PK Nicholls, T Endo, EK Jackson, SA Cobb, DG de Rooij, DC Page
    Elife, 2020-07-20;9(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  9. Biological and RNA regulatory function of MOV10 in mammalian germ cells
    Authors: K Fu, S Tian, H Tan, C Wang, H Wang, M Wang, Y Wang, Z Chen, Y Wang, Q Yue, Q Xu, S Zhang, H Li, J Xie, M Lin, M Luo, F Chen, L Ye, K Zheng
    BMC Biol., 2019-05-14;17(1):39.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-F
  10. PLZFposc-KITpos-delineated A1-A4-differentiating spermatogonia by subset and stage detection upon Bouin fixation
    Authors: RL Tang, LQ Fan
    Asian J. Androl., 2019-05-01;21(3):309-318.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  11. EXOSC10/Rrp6 is post-translationally regulated in male germ cells and controls the onset of spermatogenesis
    Authors: SP Jamin, FG Petit, C Kervarrec, F Smagulova, D Illner, H Scherthan, M Primig
    Sci Rep, 2017-11-08;7(1):15065.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  12. DDX4-EGFP transgenic rat model for the study of germline development and spermatogenesis
    Authors: K Gassei, Y Sheng, A Fayomi, P Mital, M Sukhwani, CC Lin, KA Peters, A Althouse, H Valli, KE Orwig
    Biol. Reprod., 2017-03-01;96(3):707-719.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC-P
  13. The histone demethylase UTX regulates the lineage-specific epigenetic program of invariant natural killer T cells
    Nat. Immunol, 2016-12-19;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  14. Multiple layers of transcriptional regulation by PLZF in NKT-cell development
    Authors: Ai-Ping Mao
    Proc Natl Acad Sci USA, 2016-06-20;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation
  15. CRISPR/Cas9 Promotes Functional Study of Testis Specific X-Linked Gene In Vivo.
    Authors: Li M, Huang R, Jiang X, Chen Y, Zhang Z, Zhang X, Liang P, Zhan S, Cao S, Songyang Z, Huang J
    PLoS ONE, 2015-11-24;10(11):e0143148.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  16. PAX7 expression defines germline stem cells in the adult testis.
    Authors: Aloisio G, Nakada Y, Saatcioglu H, Pena C, Baker M, Tarnawa E, Mukherjee J, Manjunath H, Bugde A, Sengupta A, Amatruda J, Cuevas I, Hamra F, Castrillon D
    J Clin Invest, 2014-08-18;124(9):3929-44.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  17. Reconstruction of mouse testicular cellular microenvironments in long-term seminiferous tubule culture.
    Authors: Makela J, Toppari J, Rivero-Muller A, Ventela S
    PLoS ONE, 2014-03-11;9(3):e90088.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  18. PLZF mediates the PTEN/AKT/FOXO3a signaling in suppression of prostate tumorigenesis.
    Authors: Cao, JingPing, Zhu, Shu, Zhou, Wei, Li, Jie, Liu, Chang, Xuan, HanQing, Yan, Jie, Zheng, Lin, Zhou, LiXin, Yu, JianXiu, Chen, GuoQiang, Huang, YiRan, Yu, Zhuo, Feng, LiXin
    PLoS ONE, 2013-12-10;8(12):e77922.
    Species: Human
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  19. BTB-ZF factors recruit the E3 ligase cullin 3 to regulate lymphoid effector programs.
    Authors: Mathew, Rebecca, Seiler, Michael, Scanlon, Seth T, Mao, Ai-ping, Constantinides, Michael, Bertozzi-Villa, Clara, Singer, Jeffrey, Bendelac, Albert
    Nature, 2012-10-21;491(7425):618-21.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: ICC, Western Blot

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