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Human ROBO3 Antibody

Catalog #: AF3076
11 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF3076
AF3076-SP
Product Details
Citations (11)
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human ROBO3 Antibody Summary

Species Reactivity
Human
Specificity
Detects ROBO3 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 60% cross-reactivity with recombinant mouse ROBO3 is observed, 5% cross-reactivity with recombinant human ROBO2 and recombinant rat ROBO1 is observed, and less than 1% cross‑reactivity with recombinant human ROBO4 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human ROBO3
Leu40-Ser545
Accession # Q96MS0
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Purity
Immunogen affinity purified

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human ROBO3 Fc Chimera (Catalog # 3076-RB)
Immunohistochemistry
5-15 µg/mL
Immersion fixed frozen sections of embryonic mouse spinal cord (E11.5)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ROBO3

Human ROBO3 is a 200 kDa member of the ROBO family of guidance molecules (1‑3). The term ROBO derives from round-about, a description of the circuitous pathway axons take in the absence of a functional ROBO gene (3, 4). Human ROBO3 is a type I transmembrane glycoprotein that is synthesized as a 1386 amino acid (aa) precursor. It contains a 20 aa signal sequence, an 871 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 474 aa cytoplasmic region (5, 6). The ECD contains five C2-type Ig-like domains (aa 64‑531) and three fibronectin (FN) type III domains (aa 555‑863). The cytoplasmic region contains three of four possible 15‑20 aa long CC (conserved cytoplasmic) motifs that are found in ROBO1 (7, 8). Human ROBO3 has multiple isoforms. An alternate start site generates a 1365 aa A isoform and a 1341 aa B isoform. These two mature forms differ only over the first 26 and 2 amino acids of the N-terminus, respectively. There are multiple point mutations and insertions in the ROBO3 gene. Three result in truncated forms. One is 456 aa in length and ends after the Ig-like domain # 4. A second is 770 aa in length and ends in the third FN domain. A third isoform is truncated after aa 1108 in the cytoplasmic region after CC2. At least one alternate splice form is also reported. It shows a 10 aa substitution between aa 1025‑1034, followed by truncation. Human ROBO3 ECD is 84% and 91% aa identical to the ECD in mouse and canine ROBO3, respectively. Normally, axons originating on one side of the spinal cord are inhibited from crossing to the other side by a SLIT2-ROBO1 interaction at the midline. ROBO3 is permissive for this event. It is unclear how this is accomplished. One possibility is that it binds directly to ROBO1, blocking SLIT activation. A second possibility involves ROBO3 binding to SLIT2 in a nonproductive interaction. However, only ROBO3 Form B is known to bind to SLIT2 (9‑11).

References
  1. Rajagopalan, S. et al. (2000) Neuron 28:767.
  2. Guthrie, S. (2004) Curr. Biol. 14:R632.
  3. Guthrie, S. (2001) Curr. Biol. 11:R300.
  4. Seeger, M. et al. (1993) Neuron 10:409.
  5. Jen, J.C. et al. (2004) Science 304:1509.
  6. Swiss-Prot. Accession # Q96MS0.
  7. Bashaw, G.J. et al. (2000) Cell 101:703.
  8. Kidd, T. et al. (1998) Cell 92:205.
  9. Camurri, L. et al. (2005) Mol. Cell. Neurosci. 30:485.
  10. Sabatier, C. et al. (2004) Cell 117:157.
  11. Mambetisaeva, E.T. et al. (2005) Dev. Dyn. 233:41.
Long Name
Roundabout Homolog 3
Entrez Gene IDs
64221 (Human); 19649 (Mouse)
Alternate Names
FLJ21044; HGPPS; HGPS; RBIG1horizontal gaze palsy with progressive scoliosis; retinoblastoma inhibiting gene 1; RIG1; ROBO3; roundabout (axon guidance receptor, Drosophila) homolog 3; roundabout homolog 3; roundabout, axon guidance receptor, homolog 3 (Drosophila); Roundabout-like protein 3

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Citations for Human ROBO3 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

11 Citations: Showing 1 - 10
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  1. Tace/ADAM17 is a bi-directional regulator of axon guidance that coordinates distinct Frazzled and Dcc receptor signaling outputs
    Authors: Y Zang, K Chaudhari, GJ Bashaw
    Cell Reports, 2022-12-06;41(10):111785.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation, Western Blot
  2. Phox2a Defines a Developmental Origin of the Anterolateral System in Mice and Humans
    Authors: RB Roome, FB Bourojeni, B Mona, S Rastegar-P, R Blain, A Dumouchel, C Salesse, WS Thompson, M Brookbank, Y Gitton, L Tessarollo, M Goulding, JE Johnson, M Kmita, A Chédotal, A Kania
    Cell Rep, 2020-11-24;33(8):108425.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC
  3. Chemokine receptor CXCR7 non-cell-autonomously controls pontine neuronal migration and nucleus formation
    Authors: Y Zhu, T Hirata, F Mackay, F Murakami
    Sci Rep, 2020-07-16;10(1):11830.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  4. Afadin Signaling at the Spinal Neuroepithelium Regulates Central Canal Formation and Gait Selection
    Authors: S Skarlatou, C Hérent, E Toscano, CS Mendes, J Bouvier, N Zampieri
    Cell Rep, 2020-06-09;31(10):107741.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  5. The Ciliary Protein Arl13b Functions Outside of the Primary Cilium in Shh-Mediated Axon Guidance
    Authors: J Ferent, S Constable, ED Gigante, PT Yam, LE Mariani, E Legué, KF Liem, T Caspary, F Charron
    Cell Rep, 2019-12-10;29(11):3356-3366.e3.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  6. CD36-Mediated Metabolic Rewiring of Breast Cancer Cells Promotes Resistance to HER2-Targeted Therapies
    Authors: WW Feng, O Wilkins, S Bang, M Ung, J Li, J An, C Del Genio, K Canfield, J DiRenzo, W Wells, A Gaur, RB Robey, JY Guo, RL Powles, C Sotiriou, L Pusztai, M Febbraio, C Cheng, WB Kinlaw, M Kurokawa
    Cell Rep, 2019-12-10;29(11):3405-3420.e5.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  7. Ndfip Proteins Target Robo Receptors for Degradation and Allow Commissural Axons to Cross the Midline in the Developing Spinal Cord
    Authors: M Gorla, C Santiago, K Chaudhari, AAK Layman, PM Oliver, GJ Bashaw
    Cell Rep, 2019-03-19;26(12):3298-3312.e4.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Immunoprecipitation
  8. The ciliopathy gene ftm/rpgrip1l controls mouse forebrain patterning via region-specific modulation of hedgehog/gli signaling
    Authors: A Andreu-Cer, I Anselme, A Karam, C Laclef, M Catala, S Schneider-
    J. Neurosci., 2019-01-28;0(0):.
    Species: Mouse
    Sample Types: Embryo
    Applications: IHC
  9. Floor-plate-derived netrin-1 is dispensable for commissural axon guidance
    Authors: C Dominici, JA Moreno-Bra, SR Puiggros, Q Rappeneau, N Rama, P Vieugue, A Bernet, P Mehlen, A Chédotal
    Nature, 2017-04-26;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  10. A-kinase Anchoring Protein 79/150 Recruits Protein Kinase C to Phosphorylate Roundabout Receptors.
    Authors: Samelson B, Gore B, Whiting J, Nygren P, Purkey A, Colledge M, Langeberg L, Dell'Acqua M, Zweifel L, Scott J
    J Biol Chem, 2015-04-16;290(22):14107-19.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  11. The chemorepellent Slit3 promotes monocyte migration.
    Authors: Geutskens SB, Hordijk PL, van Hennik PB
    J. Immunol., 2010-11-15;185(12):7691-8.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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