Human TMEM219 Antibody

Catalog #: AF7556 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF7556
AF7556-SP

Detection of Human TMEM219 by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human TMEM219 Antibody Summary

Species Reactivity

Human

Specificity

Detects human TMEM219 in direct ELISAs and Western blots.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human TMEM219
Leu41-Leu200
Accession # ACQ72822

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Immunohistochemistry

1-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human TMEM219 by Western Blot. Western blot shows lysates of human liver tissue, human skeletal muscle tissue, and human kidney tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human TMEM219 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7556) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for TMEM219 at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry

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TMEM219 in Human Prostate Gland. TMEM219 was detected in immersion fixed paraffin-embedded sections of human prostate gland using Sheep Anti-Human TMEM219 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7556) at 1 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in stromal cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TMEM219

TMEM219 (Transmembrane protein 219; also IGFBP3 Receptor) is a 30-35 kDa member of the 2-transmembrane group of the Pfam-A family of polytopic molecules. It is widely expressed, and appears to be a cell-surface receptor for IGFBP3. Only free IGFBP3 will bind to TMEM219; when complexed to IGF-I, IGFBP3 is unable to recognize the TMEM219 molecule. Ligation of TMEM219 inhibits cell proliferation and induces caspase-8 mediated apoptosis. It is suggested that procaspase-8 is normally sequestered by TMEM219. Following IGFBP3 binding, procaspase-8 is activated and released from TMEM219, resulting in the downstream activation of executioner caspases. Human TMEM219 is a 240 amino acid (aa) 2-transmembrane protein. It contains an N-terminal 20 aa cytoplasmic region, a 21 aa TM domain, a 164 aa extracellular region (aa 41-204), a second 21 aa TM domain, and a C-terminal 15 aa cytoplasmic tail. Over aa 41-200, human TMEM219 shares 82% aa sequence identity with mouse TMEM219.

Long Name

Transmembrane Protein 219

Entrez Gene IDs

124446 (Human); 68742 (Mouse)

Alternate Names

IGFBP3R; IGFBP-3R; Insulin-Like Growth Factor Binding Protein-3 Receptor; Insulin-Like Growth Factor-Binding Protein 3 Receptor; LOC124446; TMEM219; Transmembrane Protein 219