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Mouse IGFALS/ALS Antibody

Catalog #: AF1436
1 Citation Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF1436
AF1436-SP
Detection of Mouse IGFALS/ALS by Western Blot.
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Citations (1)
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Mouse IGFALS/ALS Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse IGFALS/ALS in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse IGFALS/ALS
Thr28-Cys603
Accession # P70389
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Mouse IGFALS/ALS antibody by Western Blot. View Larger

Detection of Mouse IGFALS/ALS by Western Blot. Western blot shows lysates of mouse spleen tissue, mouse liver tissue, and mouse bone marrow. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse IGFALS/ALS Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1436) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for IGFALS/ALS at approximately 80-90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IGFALS/ALS

ALS (Acid labile subunit) is an 84-86 kDa glycoprotein member of the leucine rich repeat superfamily of molecules.  It is secreted by hepatocytes in response to growth hormone stimulation, and forms a 140-150 kDa ternary complex with IGFBP3 (or IGFBP5) and IGF-I.  This complex increases the half-life of IGF-I and retains it in the circulation for use in multiple tissues.  Mature mouse ALS is 580 amino acids (aa) in length (aa 24-603).  It contains twenty-one 20-23 aa LRRs that participate in protein-protein interactions.  There are two potential isoform variants that show N-terminal extensions.  One contains a 66 aa substitution for aa 1-5, while another possesses an 89 aa substitution again for aa 1-5.  Over aa 28-603, mouse ALS shares 79% and 93% aa identity with human and rat ALS, respectively.        

Long Name
IGF-binding Protein Acid Labile Subunit
Entrez Gene IDs
3483 (Human); 16005 (Mouse)
Alternate Names
ACLSD; ALS; IGFALS

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Citation for Mouse IGFALS/ALS Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Early postnatal nutrition determines somatotropic function in mice.
    Authors: Kappeler L, De Magalhaes Filho C, Leneuve P, Xu J, Brunel N, Chatziantoniou C, Le Bouc Y, Holzenberger M
    Endocrinology, 2008-09-18;150(1):314-23.
    Species: Mouse
    Sample Types: Plasma
    Applications: Western Blot

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