Rat CXCL1/CINC-1 DuoSet ELISA Summary
* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.
This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant rat CINC-1. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet.
Product Features
- Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
- Development protocols are provided to guide further assay optimization
- Assay can be customized to your specific needs
- Economical alternative to complete kits
Kit Content
- Capture Antibody
- Detection Antibody
- Recombinant Standard
- Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)
Other Reagents Required
DuoSet Ancillary Reagent Kit 2 (5 plates): (Catalog # DY008) containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and Reagent Diluent Concentrate 2.
The components listed above may be purchased separately:
PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered
Wash Buffer: (Catalog # WA126), or 0.05% Tween® 20 in PBS, pH 7.2-7.4
Reagent Diluent: (Catalog # DY995), or 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered
Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)
Stop Solution: 2 N H2SO4 (Catalog # DY994)
Microplates: R&D Systems (Catalog # DY990)
Plate Sealers: ELISA Plate Sealers (Catalog # DY992)
Scientific Data
Product Datasheets
Preparation and Storage
Background: CXCL1/GRO alpha/KC/CINC-1
CXCL1/GRO alpha, also known as KC in mouse and CINC-1 in rat, is a member of the CXC family of chemokines. Human CXCL1/GRO alpha is 107 amino acids (aa) in length with a predicted molecular weight of 11 kDa. The mouse and rat orthologs share 57% and 59% amino acid sequence identity with the human protein, respectively. Genome-wide analysis suggests that the protein encoded by the mouse Cxcl3 gene is homologous to human CXCL1/GRO alpha protein. Human CXCL1/GRO alpha can be cleaved into three isoforms CXCL1/GRO alpha (aa 4-73), CXCL1/GRO alpha (aa 5-73), and CXCL1/GRO alpha (aa 6-73) which show higher activity than the full length protein. CXCL1/GRO alpha is secreted by mast cells and macrophages whereupon it acts as a chemoattractant for neutrophils.
Assay Procedure
GENERAL ELISA PROTOCOL
Plate Preparation
- Dilute the Capture Antibody to the working concentration in PBS without carrier protein. Immediately coat a 96-well microplate with 100 μL per well of the diluted Capture Antibody. Seal the plate and incubate overnight at room temperature.
- Aspirate each well and wash with Wash Buffer, repeating the process two times for a total of three washes. Wash by filling each well with Wash Buffer (400 μL) using a squirt bottle, manifold dispenser, or autowasher. Complete removal of liquid at each step is essential for good performance. After the last wash, remove any remaining Wash Buffer by aspirating or by inverting the plate and blotting it against clean paper towels.
- Block plates by adding 300 μL Reagent Diluent to each well. Incubate at room temperature for a minimum of 1 hour.
- Repeat the aspiration/wash as in step 2. The plates are now ready for sample addition.
Assay Procedure
- Add 100 μL of sample or standards in Reagent Diluent, or an appropriate diluent, per well. Cover with an adhesive strip and incubate 2 hours at room temperature.
- Repeat the aspiration/wash as in step 2 of Plate Preparation.
- Add 100 μL of the Detection Antibody, diluted in Reagent Diluent, to each well. Cover with a new adhesive strip and incubate 2 hours at room temperature.
- Repeat the aspiration/wash as in step 2 of Plate Preparation.
- Add 100 μL of the working dilution of Streptavidin-HRP to each well. Cover the plate and incubate for 20 minutes at room temperature. Avoid placing the plate in direct light.
- Repeat the aspiration/wash as in step 2.
- Add 100 μL of Substrate Solution to each well. Incubate for 20 minutes at room temperature. Avoid placing the plate in direct light.
- Add 50 μL of Stop Solution to each well. Gently tap the plate to ensure thorough mixing.
- Determine the optical density of each well immediately, using a microplate reader set to 450 nm. If wavelength correction is available, set to 540 nm or 570 nm. If wavelength correction is not available, subtract readings at 540 nm or 570 nm from the readings at 450 nm. This subtraction will correct for optical imperfections in the plate. Readings made directly at 450 nm without correction may be higher and less accurate.
Citations for Rat CXCL1/CINC-1 DuoSet ELISA
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
17
Citations: Showing 1 - 10
Filter your results:
Filter by:
-
Breaking the symmetry of cell contractility drives tubulogenesis via CXCL1 polarization
Authors: Kuo, CH;Lee, GH;Wu, HL;Huang, JY;Tang, MJ;
Proceedings of the National Academy of Sciences of the United States of America
Species: S. agalactiae
Sample Types: Cell Culture Supernates
-
Efficacy and Safety Evaluation of Mometasone Furoate in Treating Ocular Inflammation
Authors: NA Lage, MRB de Paiva, DV Vasconcelo, RR Machado, SL Fialho, A Silva-Cunh
Pharmaceutics, 2023-01-05;15(1):.
Species: Rat
Sample Types: Tissue Homogenates
-
Human Mesenchymal Stem Cell Secretome from Bone Marrow or Adipose-Derived Tissue Sources for Treatment of Hypoxia-Induced Pulmonary Epithelial Injury
Authors: N Shologu, M Scully, JG Laffey, D O'Toole
Int J Mol Sci, 2018-09-30;19(10):.
Species: Human
Sample Types: Cell Culture Supernates
-
Pyrrolidine dithiocarbamate administered during ex-vivo lung perfusion promotes rehabilitation of injured donor rat lungs obtained after prolonged warm ischemia
Authors: C Francioli, X Wang, R Parapanov, E Abdelnour, J Lugrin, F Gronchi, J Perentes, P Eckert, HB Ris, L Piquilloud, T Krueger, L Liaudet
PLoS ONE, 2017-03-21;12(3):e0173916.
Species: Rat
Sample Types: BALF
-
Food-grade TiO2 impairs intestinal and systemic immune homeostasis, initiates preneoplastic lesions and promotes aberrant crypt development in the rat colon
Authors: S Bettini, E Boutet-Rob, C Cartier, C Com‚ra, E Gaultier, J Dupuy, N Naud, S Tach‚, P Grysan, S Reguer, N Thieriet, M R‚fr‚giers, D ThiaudiŠre, JP Cravedi, M CarriŠre, JN Audinot, FH Pierre, L Guzylack-P, E Houdeau
Sci Rep, 2017-01-20;7(0):40373.
Species: Rat
Sample Types: Tissue Homogenates
-
Intra-articular blockade of P2X7 receptor reduces the articular hyperalgesia and inflammation in the knee joint synovitis especially in female rats
Authors: Cláudia Herrera Tambeli
J Pain, 2016-11-04;0(0):.
Species: Rat
Sample Types: Synovial Lavage Fluid
-
Activation of brain endothelial cells by interleukin-1 is regulated by the extracellular matrix after acute brain injury.
Authors: Summers L, Kangwantas K, Rodriguez-Grande B, Denes A, Penny J, Kielty C, Pinteaux E
Mol Cell Neurosci, 2013-10-24;57(0):93-103.
Species: Rat
Sample Types: Cell Lysates
-
Action at a distance: systemically administered adult stem/progenitor cells (MSCs) reduce inflammatory damage to the cornea without engraftment and primarily by secretion of TNF-alpha stimulated gene/protein 6.
Authors: Roddy GW, Oh JY, Lee RH, Bartosh TJ, Ylostalo J, Coble K, Rosa RH, Prockop DJ
Stem Cells, 2011-10-01;29(10):1572-9.
Species: Rat
Sample Types: Tissue Homogenates
-
TNF-alpha, IL-1beta, IL-6, and cinc-1 levels in rat brain after meningitis induced by Streptococcus pneumoniae.
Authors: Barichello T, dos Santos I, Savi GD, Simoes LR, Silvestre T, Comim CM, Sachs D, Teixeira MM, Teixeira AL, Quevedo J
J. Neuroimmunol., 2010-03-03;221(1):42-5.
Species: Rat
Sample Types: Tissue Homogenates
-
Effect of fudosteine on mucin production.
Authors: Rhee CK, Kang CM, You MB, Yoon HK, Kim YK, Kim KH, Moon HS, Park SH, Song JS
Eur. Respir. J., 2008-06-25;32(5):1195-202.
Species: Rat
Sample Types: BALF
-
Novel role for the liver X nuclear receptor in the suppression of lung inflammatory responses.
Authors: Birrell MA, Catley MC, Hardaker E, Wong S, Willson TM, McCluskie K, Leonard T, Farrow SN, Collins JL, Haj-Yahia S, Belvisi MG
J. Biol. Chem., 2007-08-31;282(44):31882-90.
Species: Rat
Sample Types: Tissue Homogenates
-
Sirtuin regulates cigarette smoke-induced proinflammatory mediator release via RelA/p65 NF-kappaB in macrophages in vitro and in rat lungs in vivo: implications for chronic inflammation and aging.
Authors: Yang SR, Wright J, Bauter M, Seweryniak K, Kode A, Rahman I
Am. J. Physiol. Lung Cell Mol. Physiol., 2006-10-13;292(2):L567-76.
Species: Rat
Sample Types: BALF
-
Utility of exhaled nitric oxide as a noninvasive biomarker of lung inflammation in a disease model.
Authors: Birrell MA, McCluskie K, Hardaker E, Knowles R, Belvisi MG
Eur. Respir. J., 2006-09-27;28(6):1236-44.
Species: Rat
Sample Types: BALF
-
Inhibition of angiogenesis by interleukin-4 gene therapy in rat adjuvant-induced arthritis.
Authors: Haas CS, Amin MA, Allen BB, Ruth JH, Haines GK, Woods JM, Koch AE
Arthritis Rheum., 2006-08-01;54(8):2402-14.
Species: Rat
Sample Types: Tissue Homogenates
-
Alveolar macrophages contribute to alveolar barrier dysfunction in ventilator-induced lung injury.
Authors: Frank JA, Wray CM, McAuley DF, Schwendener R, Matthay MA
Am. J. Physiol. Lung Cell Mol. Physiol., 2006-07-28;291(6):L1191-8.
Species: Rat
Sample Types: Plasma
-
Cardiac inflammation contributes to right ventricular dysfunction following experimental pulmonary embolism in rats.
Authors: Watts JA, Zagorski J, Gellar MA, Stevinson BG, Kline JA
J. Mol. Cell. Cardiol., 2006-06-30;41(2):296-307.
Species: Rat
Sample Types: Tissue Homogenates
-
Differential effects of ebselen on neutrophil recruitment, chemokine, and inflammatory mediator expression in a rat model of lipopolysaccharide-induced pulmonary inflammation.
Authors: Haddad el-B, McCluskie K, Birrell MA, Dabrowski D, Pecoraro M, Underwood S, Chen B, De Sanctis GT, Webber SE, Foster ML, Belvisi MG
J. Immunol., 2002-07-15;169(2):974-82.
Species: Rat
Sample Types: Tissue Homogenates
FAQs
No product specific FAQs exist for this product, however you may
View all ELISA FAQsReviews for Rat CXCL1/CINC-1 DuoSet ELISA
Average Rating: 4.3 (Based on 3 Reviews)
Have you used Rat CXCL1/CINC-1 DuoSet ELISA?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
Filter by:
