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Recombinant Human CXCL17/VCC-1 Protein

Catalog #: 4207-DM
3 Citations Datasheet / COA / SDS

Carrier Free

Catalog # Availability Size / Price Qty
4207-DM-025/CF

With Carrier

Catalog # Availability Size / Price Qty
4207-DM-025
R&D Systems Recombinant Proteins and Enzymes
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Product Details
Citations (3)
FAQs
Reviews
Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human CXCL17/VCC-1 Protein Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to induce VEGF expression in mouse endothelial cells. Weinstein, E.J. et al. (2006) BBRC 350:74. The ED50 for this effect is 1-5 µg/mL.
Source
E. coli-derived human CXCL17/VCC-1 protein
Leu24-Leu119
Accession #
NP_940879
N-terminal Sequence
Analysis
Leu24
Predicted Molecular Mass
11.3 kDa

Product Datasheets

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4207-DM (with carrier)

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4207-DM/CF (carrier free)

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COA
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Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

4207-DM

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

4207-DM/CF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CXCL17/VCC-1

Dendritic cell and monocyte chemokine-like protein (DMC), also known as VEGF-correlated chemokine-1 (VCC-1) and CXCL17, is a novel secreted molecule that belongs to the intercrine alpha (or CXC) family of chemokines (1, 2). It has no predicted N‑glycosylation sites, so cleavage of a 22 amino acid (aa) signal sequence likely results in a mature human DMC of 97 aa and 11 kDa. DMC is constitutively produced by bronchiolar, mammary, and intestinal epithelium, vascular endothelial cells, and multiple tumor types (1, 2, 3). It induces the chemotaxis of quiescent, but not LPS-activated, peripheral blood monocytes and dendritic cells, and also is noted to specifically bind to these cells (1). DMC expression is increased in endothelial cells when they are induced to form tubes in vitro (2). Transgenic overexpression in NIH3T3 cells causes up‑regulation of proteins such as VEGF and FGF basic, and increases cell growth rate and tumorigenicity (2). DMC and two other chemokines that play roles in angiogenesis, CXCL1/GRO and CXCL8/IL-8, show significantly correlated expression with that of VEGF in primary lung, breast and esophageal tumors (2). DMC is therefore suggested to play a role in tumor angiogenesis. Mature human DMC shares 73%, 71% and 64% amino acid sequence identity with bovine, mouse and rat DMC, respectively.

References
  1. Pisabarro, M. T. et al. (2006) J. Immunol. 176:2069.
  2. Weinstein, E. J. et al. (2006) Biochem. Biophys. Res. Commun. 350:74.
  3. Mu, X. et al. (2009) Acta Biochim. Biophys. Sin 41:632.
Entrez Gene IDs
284340 (Human); 232983 (Mouse)
Alternate Names
Chemokine (C-X-C Motif) Ligand 17; C-X-C Motif Chemokine 17; CXCL17; Dcip1; Dendritic Cell And Monocyte Chemokine-Like Protein; DMC; UNQ473; VCC1; VCC-1; VEGF Coregulated Chemokine 1; VEGF Co-Regulated Chemokine 1

Citations for Recombinant Human CXCL17/VCC-1 Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Lysophosphatidic Acid-Mediated GPR35 Signaling in CX3CR1+ Macrophages Regulates Intestinal Homeostasis
    Authors: B Kaya, C Doñas, P Wuggenig, OE Diaz, RA Morales, H Melhem, PP Hernández, T Kaymak, S Das, P Hruz, Y Franc, F Geier, CK Ayata, EJ Villablanc, JH Niess
    Cell Rep, 2020-08-04;32(5):107979.
    Species: Chinese Hamster, Hamster
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Cutting edge: GPR35/CXCR8 is the receptor of the mucosal chemokine CXCL17.
    Authors: Maravillas-Montero J, Burkhardt A, Hevezi P, Carnevale C, Smit M, Zlotnik A
    J Immunol, 2014-11-19;194(1):29-33.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors.
    Authors: Wilson TR, Fridlyand J, Yan Y, Penuel E, Burton L, Chan E, Peng J, Lin E, Wang Y, Sosman J, Ribas A, Li J, Moffat J, Sutherlin DP, Koeppen H, Merchant M, Neve R, Settleman J
    Nature, 2012-07-26;487(7408):505-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay

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