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Recombinant Human His6UBE2S Protein, CF

Catalog #: E2-690
4 Citations Datasheet / COA / SDS

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Discontinued Product

E2-690 has been discontinued.
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Citations (4)
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Recombinant Human His6UBE2S Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.
Activity
Recombinant Human His6-UBE2S is a member of the Ubiquitin-conjugating (E2) enzyme family that receives Ubiquitin from a Ubiquitin-activating (E1) enzyme and subsequently interacts with a Ubiquitin ligase (E3) to conjugate Ubiquitin to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human His6-UBE2S concentration of 0.1-1 μM.
Source
E. coli-derived human UBE2S protein
Met1 - Leu222 with a N-terminal 6-His tag
Accession #
NP_055316.2
Predicted Molecular Mass
26 kDa

Product Datasheets

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E2-690

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

E2-690

Formulation Supplied as a 0.2 μm filtered solution in HEPES, NaCl, TCEP and Glycerol.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: UBE2S

Ubiquitin-conjugating Enzyme E2S (UBE2S), also known as E2-EPF, is a widely expressed protein with a predicted molecular weight of 26 kDa (1). Human UBE2S shares 92% and 93% amino acid sequence identity with mouse and rat UBE2S, respectively. UBE2S expression oscillates in a cell cycle-dependent fashion, and it influences cell cycle progression by mediating the Ubiquitin-dependent proteolysis of mitotic regulators (2-4). UBE2S interacts with APC/C, a Ubiquitin ligase (E3), to catalyze the elongation of Lys11-linked Ubiquitin chains on APC/C substrates that have been mono-ubiquitinated by UbcH10/UBE2C or UbcH5/UBE2D (3-7). UBE2S is overexpressed in multiple human cancers and targets the tumor suppressor pVHL for proteasomal degradation, which stabilizes HIF-1 alpha and promotes tumor progression (8).

References
  1. Liu, Z. et al. (1992) J. Biol. Chem. 267:15829.
  2. Tedesco, D. et al. (2007) Neoplasia 9:601.
  3. Williamson, A. et al. (2009) Proc. Natl. Acad. Sci. USA 106:18213.
  4. Dimova, N.V. et al. (2012) Nat. Cell Biol. 14:168.
  5. Garnett, M.J. et al. (2009) Nat. Cell Biol. 11:1363.
  6. Wu, T. et al. (2010) Proc. Natl. Acad. Sci. USA 107:1355.
  7. Bremm, A. & D. Komander (2012) Methods Mol. Biol. 832:219.
  8. Jung, C.R. et al. (2006) Nat. Med. 12:809.

Long Name
Ubiquitin-conjugating Enzyme E2S
Entrez Gene IDs
27338 (Human); 292588 (Rat)
Alternate Names
E2-24K; E2-EPF; E2-EPF5; E2-EPF52; E2-EPFEC 6.3.2.19; E2EPFEPF5; EPF5; UBE2S; Ubiquitin carrier protein S; ubiquitin-conjugating enzyme E2 S; ubiquitin-conjugating enzyme E2-24 kD; Ubiquitin-conjugating enzyme E2-24 kDa; Ubiquitin-conjugating enzyme E2-EPF5; ubiquitin-conjugating enzyme E2S; Ubiquitin-protein ligase S

Citations for Recombinant Human His6UBE2S Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Proteasomal degradation of the tumour suppressor FBW7 requires branched ubiquitylation by TRIP12
    Authors: OM Khan, J Almagro, JK Nelson, S Horswell, V Encheva, KS Keyan, BE Clurman, AP Snijders, A Behrens
    Nature Communications, 2021-04-06;12(1):2043.
    Species: Human
    Sample Types: Protein
    Applications: Bioassay
  2. piRNA-independent function of PIWIL1 as a co-activator for anaphase promoting complex/cyclosome to drive pancreatic cancer metastasis
    Authors: F Li, P Yuan, M Rao, CH Jin, W Tang, YF Rong, YP Hu, F Zhang, T Wei, Q Yin, T Liang, L Wu, J Li, D Li, Y Liu, W Lou, S Zhao, MF Liu
    Nat. Cell Biol., 2020-03-16;0(0):.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Bioassay
  3. Site-specific ubiquitylation and SUMOylation using genetic-code expansion and sortase
    Authors: M Fottner, AD Brunner, V Bittl, D Horn-Ghetk, A Jussupow, VRI Kaila, A Bremm, K Lang
    Nat. Chem. Biol., 2019-02-15;15(3):276-284.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Enzyme Assay
  4. Neddylation E2 UBE2F promotes the survival of lung cancer cells by activating CRL5 to degrade NOXA via the K11 linkage
    Clin Cancer Res, 2016-09-02;0(0):.
    Species: Human
    Sample Types: Protein
    Applications: Ubiquitination

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