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Recombinant Human UBE2K/E2-25K Protein, CF

Catalog #: SP-200
1 Citation Datasheet / COA / SDS

Discontinued Product

SP-200 has been discontinued.
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Recombinant Human UBE2K/E2-25K Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain
Activity
Recombinant Human UBE2K/E2-25K is ideal for use as a control substrate for in vitro SUMO conjugation. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human UBE2K/E2-25K concentration of 1-5 μM.
Source
E. coli-derived human Miro1/Rhot1 protein
Accession #
P61086
Predicted Molecular Mass
22 kDa

Product Datasheets

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SP-200

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

SP-200

Formulation X mg/ml (X μM) in 50 mM HEPES pH 7.5, 150 mM NaCl, 1 mM TCEP
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Miro1/Rhot1

Ubiquitin-conjugating Enzyme E2K (UBE2K), also known as E2-25K, HIP2, and LIG, is a 200 amino acid (aa) protein with a predicted molecular weight of 25 kDa. Human and mouse UBE2K share 100% aa sequence identity. Similar to other Ubiquitin-conjugating (E2) enzymes, UBE2K/E2-25K contains a conserved Ubiquitin-conjugating domain but is unique in that it also has a C-terminal, non-covalent Ubiquitin binding surface termed the Ubiquitin-associated domain between aa residues 160 and 200 (1,2). UBE2K/E2-25K mediates the elongation of Lys48-linked poly-Ubiquitin chains (3).UBE2K/E2-25K catalytic activity can be modulated by the post-translational addition of SUMO at Lys14 (4). Substrates include the Huntingtin protein and the tumor suppressor RB1 (5,6). UBE2K/E2-25K is widely expressed, with highest levels found in the brain cortex and striatum, and dysregulated UBE2K/E2-25K is implicated in polyglutamine diseases and Alzheimer's disease (7-9).

References
  1. Wilson, R.C. et al. (2011) Biochem. Biophys. Res. Commun. 405:662.
  2. Haldeman, M.T. et al. (1997) Biochemistry 36:10526.
  3. Chen, Z.J. et al. (1991) J. Biol. Chem. 266:15698.
  4. Pickler, A. et al. (2005) Nat. Struct. Mol. Biol. 12:264.
  5. Kalchman, M.A. et al. (1996) J. Biol. Chem. 271:19385.
  6. Oh, K.J. et al. (2010) Virology 396:118.
  7. de Pril, R. et al. (2007) Mol. Cell. Neurosci. 34:10.
  8. Skibinski, G.A. & L. Boyd (2012) BMC Cell Biol. 13:10.
  9. Song, S. et al. (2003) Mol. Cell 12:553.
Long Name
Mitochondrial Rho (MIRO) GTPase 1
Entrez Gene IDs
55288 (Human)
Alternate Names
ARHT1; EC 3.6.5; EC 3.6.5.-; FLJ11040; FLJ12633; hMiro-1; Miro1; MIRO-1mitochondrial Rho GTPase 1; mitochondrial Rho 1; rac-GTP binding protein-like protein; Rac-GTP-binding protein-like protein; Ras homolog gene family member T1; ras homolog gene family, member T1; Rhot1

Citation for Recombinant Human UBE2K/E2-25K Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Using glycyrrhizic acid to target sumoylation processes during Epstein-Barr virus latency
    Authors: GL Bentz, AJ Lowrey, DC Horne, V Nguyen, AR Satterfiel, TD Ross, AE Harrod, ON Uchakina, RJ McKallip
    PLoS ONE, 2019-05-24;14(5):e0217578.
    Species: Human
    Sample Types: Cell Lysates

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