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Canine/Equine CD44 Antibody

Catalog #: MAB5449
8 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB5449
MAB5449-SP
Detection of CD44 in Canine Monocytes by Flow Cytometry.
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Citations (8)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Canine/Equine CD44 Antibody Summary

Species Reactivity
Canine, Equine
Specificity
Detects canine and equine CD44 in Flow Cytometry.
Source
Monoclonal Mouse IgG1 Clone # 69-S5
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Canine marrow cells
Accession # Q28284
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Purity
Protein A or G purified from hybridoma culture supernatant

Applications

Recommended Concentration
Sample
Flow Cytometry
2.5 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of CD44 antibody in Canine Monocytes antibody by Flow Cytometry. View Larger

Detection of CD44 in Canine Monocytes by Flow Cytometry. Canine blood-derived monocytes were stained with Mouse Anti-Canine/Equine CD44 Monoclonal Antibody (Catalog # MAB5449, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (Catalog # F0101B).

Flow Cytometry Detection of CD44 antibody in Equine PBMCs antibody by Flow Cytometry. View Larger

Detection of CD44 in Equine PBMCs by Flow Cytometry. Equine peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Canine/Equine CD44 Monoclonal Antibody (Catalog # MAB5449, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD44

Canine CD44 is a ubiquitously expressed 85‑90 kDa transmembrane glycoprotein that binds to hyaluronan and is involved in matrix adhesion, lymphocyte activation, and lymph node homing. The CD44 protein is expressed as a family of molecular isoforms generated by alternate splicing and variable posttranslational modification. Within the N‑terminal invariant portion of the ECD (aa 14‑191), canine CD44 shares 90%, 83%, and 82% identity with human, mouse, and rat CD44, respectively.

Entrez Gene IDs
960 (Human); 12505 (Mouse); 25406 (Rat); 100126860 (Porcine)
Alternate Names
CD44 antigen; CD44 molecule (Indian blood group); CD44; CD44R; CDw44; cell surface glycoprotein CD44; chondroitin sulfate proteoglycan 8; CSPG8; ECMR-III; epican; Extracellular matrix receptor III; GP90 lymphocyte homing/adhesion receptor; HCAM; HCELL; hematopoietic cell E- and L-selectin ligand; Heparan sulfate proteoglycan; Hermes antigen; homing function and Indian blood group system; HUTCH-I; Hyaluronate receptor; IN; LHR; MC56; MDU2; MDU2CD44 antigen (homing function and Indian blood group system); MDU3; MDU3CDW44; MIC4; MIC4MGC10468; MUTCH-I; Pgp1; PGP-1; PGP-I; Phagocytic glycoprotein 1; Phagocytic glycoprotein I

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Citations for Canine/Equine CD44 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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  1. Cladophora glomerata methanolic extract promotes chondrogenic gene expression and cartilage phenotype differentiation in equine adipose-derived mesenchymal stromal stem cells affected by metabolic syndrome
    Authors: L Bourebaba, I Michalak, M Baouche, K Kucharczyk, K Marycz
    Stem Cell Res Ther, 2019-12-17;10(1):392.
    Species: Equine
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. 5-Azacytidine and Resveratrol Enhance Chondrogenic Differentiation of Metabolic Syndrome-Derived Mesenchymal Stem Cells by Modulating Autophagy
    Authors: K Marycz, JMI Houston, C Weiss, M Röcken, K Kornicka
    Oxid Med Cell Longev, 2019-05-12;2019(0):1523140.
    Species: Equine
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. 5-Azacytydine and resveratrol reverse senescence and ageing of adipose stem cells via modulation of mitochondrial dynamics and autophagy
    Authors: K Kornicka, J Sz?apka-Ko, A ?mieszek, K Marycz
    J. Cell. Mol. Med., 2018-10-28;23(1):237-259.
    Species: Equine
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Combination of resveratrol and 5-azacytydine improves osteogenesis of metabolic syndrome mesenchymal stem cells
    Authors: K Marycz, K Kornicka, JM Irwin-Hous, C Weiss
    J. Cell. Mol. Med., 2018-07-12;0(0):.
    Species: Equine
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Evaluation of Oxidative Stress and Mitophagy during Adipogenic Differentiation of Adipose-Derived Stem Cells Isolated from Equine Metabolic Syndrome (EMS) Horses
    Authors: K Marycz, C Weiss, A ?mieszek, K Kornicka
    Stem Cells Int, 2018-06-06;2018(0):5340756.
    Species: Equine
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. Macroautophagy and Selective Mitophagy Ameliorate Chondrogenic Differentiation Potential in Adipose Stem Cells of Equine Metabolic Syndrome: New Findings in the Field of Progenitor Cells Differentiation
    Authors: K Marycz, K Kornicka, J Grzesiak, A ?mieszek, J Sz?apka
    Oxid Med Cell Longev, 2016-12-08;2016(0):3718468.
    Applications: Flow Cytometry
  7. Equine metabolic syndrome impairs adipose stem cells osteogenic differentiation by predominance of autophagy over selective mitophagy
    J Cell Mol Med, 2016-09-14;0(0):.
    Species: Equine
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  8. Canine and Equine Mesenchymal Stem Cells Grown in Serum Free Media Have Altered Immunophenotype.
    Authors: Clark K, Kol A, Shahbenderian S, Granick J, Walker N, Borjesson D
    Stem Cell Rev, 2016-04-01;12(2):245-56.
    Species: Canine
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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