FAI

Catalog # Availability Size / Price Qty
8011/50
FAI | CAS No. 1415238-77-5 | DNA, RNA and Protein Synthesis Compounds
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Description: Reagent for RNA SHAPE-MaP experiments in live cells

Chemical Name: 1H-Imidazol-1-yl(2-methyl-3-furanyl)methanone

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Biological Activity

FAI is a reagent for RNA SHAPE-MaP experiments in live cells. Can be used to map RNA structures in vivo by forming stable 2'-OH adducts with RNA, blocking reverse transcriptase elongation. In vitro, FAI activity can be quenched with Dithiothreitol (Cat. No. 3154) and can be used to probe the secondary structure of mouse embryonic stem cell 5S rRNA. At elevated temperatures, FAI forms more stable adducts and exhibits less hydrolysis than NAI (Cat. No 7004).

Technical Data

M.Wt:
176.17
Formula:
C9H8N2O2
Solubility:
Soluble to 100 mM in DMSO
Storage:
Store at -20°C
CAS No:
1415238-77-5

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.

Background References

  1. RNA SHAPE analysis in living cells.
    Spitale et al.
    Nat.Chem.Biol., 2013;9:18

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