NAI

Biological Activity

NAI is a reagent for RNA SHAPE-MaP experiments in live cells. Acylates the 2'-OH in RNA, labels single stranded or flexible regions more than folded regions (SHAPE methodology). Enables analysis of RNA secondary structures at single nucleotide resolution. Exhibits greater signals with lower background than 1M7 for in vivo measurement of the RNA structurome. Also more stable than 1M7 in water. β-mercaptoethanol terminates reaction. Cell permeable.

Technical Data

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.

Background References

1. Comparison of SHAPE reagents for mapping RNA structures inside living cells.
   Lee et al.
   RNA, 2017;23:169
2. Determination of in vivo RNA structure in low-abundance transcripts.
   Kwok et al.
   Nat.Commun., 2013;4:2971
3. RNA SHAPE analysis in living cells.
   Spitale et al.
   Nat.Chem.Biol., 2013;9:18

Product Datasheets