Human ACE-2 DuoSet ELISA

Catalog #: DY933-05
11 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
DY933-05

Ancillary Products Available

DY008B: DuoSet ELISA Ancillary Reagent Kit 2

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All ACE-2 ELISAs

Product Details

Citations (11)

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Summary Product Features Kit Content Other Reagents Required Data Examples Product Datasheets Preparation and Storage Background

Human ACE-2 DuoSet ELISA Summary

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Sample Volume Required

100 µL

Assay Range

0.3 - 20 ng/mL

Sufficient Materials

For five 96-well plates*

Specificity

Please see the product datasheet

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human Angiotensin Converting Enzyme 2 (ACE-2). The suggested diluent is suitable for the analysis of most cell culture supernate, cell lysate, serum, plasma, and urine samples. Diluents for complex matrices, such astissue lysates, serum, plasma, and urine, should be evaluated prior to use in this DuoSet.

Product Features

Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
Development protocols are provided to guide further assay optimization
Assay can be customized to your specific needs
Economical alternative to complete kits

Kit Content

Capture Antibody
Detection Antibody
Recombinant Standard
Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

DuoSet Ancillary Reagent Kit 2 (5 plates): (Catalog # DY008) containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and Reagent Diluent Concentrate 2.

The components listed above may be purchased separately:

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na₂HPO₄, 1.5 mM KH₂PO₄, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Reagent Diluent*

Blocking Buffer*

Substrate Solution: 1:1 mixture of Color Reagent A (H₂O₂) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H₂SO₄ (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990), or equivalent

Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent

*For the Reagent Diluent and Blocking Buffer recommended for a specific DuoSet ELISA Development Kit, please see the product .

Scientific Data

N/A

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Human ACE-2 Standard Curve

Product Datasheets

Product Datasheet

COA

SDS

Preparation and Storage

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: ACE-2

Angiotensin converting enzyme 2 (ACE-2), also known as ACEH (ACE homolog), is an integral membrane protein and a zinc metalloprotease of the ACE family. The predicted human ACE-2 protein sequence consists of 805 amino acids, including a N-terminal signal peptide, a single catalytic domain, a C-terminal membrane anchor, and a short cytoplasmic tail. ACE-2 cleaves angiotensins I and II as a carboxypeptidase. ACE-2 has been shown to be a functional receptor of the human coronaviruses SARS-CoV and SARS-CoV-2 . Amongst ongoing research into the cellular entry mechanism of coronavirus via ACE-2, an anti-human ACE-2 antibody (R&D Systems Catalog # AF933) has blocked this interaction.

Long Name:

Angiotensin I Converting Enzyme 2

Entrez Gene IDs:

59272 (Human); 70008 (Mouse); 302668 (Rat); 100144303 (Porcine); 480847 (Canine); 418623 (Chicken); 102130864 (Cynomolgus Monkey); 554349 (Feline); 101673097 (Ferret); 101823817 (Hamster); 108390919 (Malayan Pangolin)

Alternate Names:

ACE2; ACE-2; ACEH; ACEHangiotensin I converting enzyme 2; ACE-related carboxypeptidase; angiotensin I converting enzyme (peptidyl-dipeptidase A) 2; angiotensin-converting enzyme 2; Angiotensin-converting enzyme homolog; DKFZp434A014; EC 3.4.17; EC 3.4.17.23; Metalloprotease MPROT15

Citations for Human ACE-2 DuoSet ELISA

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

11 Citations: Showing 1 - 10
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Neutrophil Elastase decreases SARS-CoV-2 Spike protein binding to human bronchial epithelia by clipping ACE-2 ectodomian from the epithelial surface
Authors: Kummarapurugu, AB;Hawkridge, AM;Ma, J;Osei, S;Martin, RK;Zheng, S;Voynow, JA;
The Journal of biological chemistry
Species: Human
Sample Types: Plasma, Sputum Supernates
Thraustochytrid hosts for expression of proteins relevant to SARS-CoV-2 intervention
Authors: J Dahmen, A Vermeulen, S Payne, C Lippmeier
PLoS ONE, 2023-04-12;18(4):e0283592.
Species: Human
Sample Types: Cell Culture Supernates
Thraustochytrid hosts for expression of proteins relevant to SARS-CoV-2 intervention
Authors: J Dahmen, A Vermeulen, S Payne, C Lippmeier
PLoS ONE, 2023;18(4):e0283592.
Species: Human
Sample Types: Cell Culture Supernates
Long Chain N3-PUFA Decreases ACE2 Protein Levels and Prevents SARS-CoV-2 Cell Entry
Authors: S Huang, CG Taylor, P Zahradka
International Journal of Molecular Sciences, 2022-11-10;23(22):.
Species: Human
Sample Types: Plasma
Metalloprotease-Dependent S2'-Activation Promotes Cell-Cell Fusion and Syncytiation of SARS-CoV-2
Authors: JV Harte, SL Wakerlin, AJ Lindsay, JV McCarthy, C Coleman-Va
Viruses, 2022-09-21;14(10):.
Species: Human
Sample Types: Cell Culture Supernates
Endothelial dysfunction and altered endothelial biomarkers in patients with post-COVID-19 syndrome and chronic fatigue syndrome (ME/CFS)
Authors: M Haffke, H Freitag, G Rudolf, M Seifert, W Doehner, N Scherbakov, L Hanitsch, K Wittke, S Bauer, F Konietschk, F Paul, J Bellmann-S, C Kedor, C Scheibenbo, F Sotzny
Journal of Translational Medicine, 2022-03-22;20(1):138.
Species: Human
Sample Types: Serum
Oxidative stress-induced endothelial dysfunction and decreased vascular nitric oxide in COVID-19 patients
Authors: V Montiel, I Lobysheva, L Gérard, M Vermeersch, D Perez-Morg, T Castelein, JB Mesland, P Hantson, C Collienne, D Gruson, MA van Dievoe, A Persu, C Beauloye, M Dechamps, L Belkhir, A Robert, M Derive, PF Laterre, AHJ Danser, X Wittebole, JL Balligand
EBioMedicine, 2022-02-23;77(0):103893.
Species: Human
Sample Types: Serum
Whole Exome Sequencing Identifies a Rare Mutation in NACAD as a Possible Cause of COVID Orchitis in Brothers
Authors: R Reddy, I Efimenko, W Chertman, T Kohn, P Diaz, D Seetharam, K Khodamorad, E Kresch, R Ramasamy
Urology, 2021-10-20;0(0):.
Species: Human
Sample Types: Plasma
Clinical grade ACE2 as a universal agent to block SARS-CoV-2 variants
Authors: G Wirnsberge, V Monteil, B Eaton, E Postnikova, M Murphy, B Braunsfeld, I Crozier, F Kricek, J Niederhöfe, A Schwarzböc, H Breid, A Sanchez Ji, A Bugajska-S, A Dohnal, C Ruf, R Gugenberge, A Hagelkruys, N Montserrat, MR Holbrook, C Oostenbrin, RH Shoemaker, A Mirazimi, JM Penninger
bioRxiv : the preprint server for biology, 2021-09-10;0(0):.
Species: Human
Sample Types: Virus
Applications: ELISA Capture
Circulating Soluble ACE2 and Upstream microRNA Expressions in Serum of Type 2 Diabetes Mellitus Patients
Authors: NM Elemam, H Hasswan, H Aljaibeji, N Sulaiman
International Journal of Molecular Sciences, 2021-05-17;22(10):.
Species: Human
Sample Types: Serum

Hypoxia induces expression of angiotensin-converting enzyme II in alveolar epithelial cells: Implications for the pathogenesis of acute lung injury in COVID-19
Authors: A Sturrock, E Zimmerman, M Helms, TG Liou, R Paine
Physiological Reports, 2021-05-01;9(9):e14854.
Species: Human
Sample Types: Cell Lysates