DuoSet ELISA Ancillary Reagent Kit 2
DuoSet ELISA Ancillary Reagent Kit 2 Summary
DuoSet Ancillary Kits provide a comprehensive collection of reagents and plates to perform five 96-well assays. DuoSet Ancillary Kit 2 contains Reagent Diluent 2. Each analyte presents varying requirements for optimal assay performance. Please consult the product datasheet for each DuoSet ELISA Development System to determine the required Reagent Diluent formulation.
- Complete kit of supplemental supplies provides an economical way to assay five 96-well ELISA plates.
- For use with DuoSets requiring the following Reagent Diluent formulation: 1% BSA in PBS, pH 7.2-7.4, 0.2 micron filtered.
- Pre-made buffers reduce preparation time and ensure optimal results.
- Clear Polystyrene Microplates (five 12x8-well strip microplates)
- ELISA Plate Sealers (24 plate sealers)
- Color Reagent A (1X, three 12.5 mL vials)
- Color Reagent B (1X, three 12.5 mL vials)
- Stop Solution (1X, three 11 mL vials)
- ELISA Plate-coating Buffer (1X, one 60 mL vial)
- Reagent Diluent Concentrate 2 (10X, two 21 mL vials)
- Wash Buffer (25X, six 21 mL vials)
Specifications
Product Datasheets
Citations for DuoSet ELISA Ancillary Reagent Kit 2
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Kallistatin deficiency exacerbates neuronal damage after cardiac arrest
Authors: Kim, H;Suh, GJ;Kwon, WY;Kim, KS;Jung, YS;Kim, T;Park, H;
Scientific reports 2024-02-21
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Crosstalk of protein clearance, inflammasome, and Ca2+ channels in retinal pigment epithelium derived from age-related macular degeneration patients
Authors: Karema-Jokinen, V;Koskela, A;Hytti, M;Hongisto, H;Viheri�l�, T;Liukkonen, M;Torsti, T;Skottman, H;Kauppinen, A;Nymark, S;Kaarniranta, K;
The Journal of biological chemistry 2023-05-01
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Identifying a selective inhibitor of autophagy that targets ATG12-ATG3 protein-protein interaction
Authors: Nuta, GC;Gilad, Y;Goldberg, N;Meril, S;Bahlsen, M;Carvalho, S;Kozer, N;Barr, H;Fridmann Sirkis, Y;Herc�k, K;B?ehov�, P;Nencka, R;Bialik, S;Eisenstein, M;Kimchi, A;
Autophagy 2023-02-22
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Increased Serum Levels of Tumor Necrosis Factor-like Ligand 1A in Atopic Dermatitis
Authors: T Hisamoto, H Suga, A Yoshizaki-, S Sato, A Yoshizaki
International Journal of Molecular Sciences, 2023-01-17;24(3):. 2023-01-17
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Nesfatin-1 in Human Milk and Its Association with Infant Anthropometry
Authors: KD Honoré, S Bruun, LN Jacobsen, M Domellöf, KF Michaelsen, S Husby, G Zachariass
Nutrients, 2022-12-30;15(1):. 2022-12-30
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RANKL neutralisation prevents osteoclast activation in a human in vitro ameloblastoma-bone model
Authors: J Pape, D Bakkalci, RA Hosni, BS Simpson, K Heikinheim, S Fedele, U Cheema
Journal of tissue engineering, 2022-12-24;13(0):2041731422114. 2022-12-24
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TRAIL receptors promote constitutive and inducible IL-8 secretion in non-small cell lung carcinoma
Authors: F Favaro, F Luciano-Ma, J Moreno-Cac, M Hernández-, D Both, C Montironi, F Püschel, E Nadal, E Eldering, C Muñoz-Pine
Cell Death & Disease, 2022-12-15;13(12):1046. 2022-12-15
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Investigation of the Exometabolomic Profiles of Rat Islets of Langerhans Cultured in Microfluidic Biochip
Authors: A Essaouiba, R Jellali, F Gilard, B Gakière, T Okitsu, C Legallais, Y Sakai, E Leclerc
Metabolites, 2022-12-15;12(12):. 2022-12-15
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Identification of Potential microRNA Panels for Male Non-Small Cell Lung Cancer Identification Using Microarray Datasets and Bioinformatics Methods
Authors: A Harangu?, R Lajos, L Budisan, O Zanoaga, C Ciocan, C Bica, R Pirlog, I Simon, M Simon, C Braicu, I Berindan-N
Journal of personalized medicine, 2022-12-13;12(12):. 2022-12-13
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Intermittent fasting increases growth differentiation factor 15 in females with overweight or obesity but not associated with food intake
Authors: K Liu, B Liu, GA Wittert, CH Thompson, AT Hutchison, LK Heilbronn
Obesity research & clinical practice, 2022-12-09;0(0):. 2022-12-09
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Eribulin normalizes pancreatic cancer-associated fibroblasts by simulating selected features of TGFbeta inhibition
Authors: T Luong, E Cukierman
BMC Cancer, 2022-12-02;22(1):1255. 2022-12-02
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On the Bioactivity of Echinacea purpurea Extracts to Modulate the Production of Inflammatory Mediators
Authors: SF Vieira, VMF Gonçalves, CP Llaguno, F Macías, ME Tiritan, RL Reis, H Ferreira, NM Neves
Oncogene, 2022-11-06;23(21):. 2022-11-06
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IV-1 gp120-CXCR4 recognition probed with synthetic nanomolar affinity D-peptides containing fragments of gp120 V3 loop
Authors: R Zhu, Q Meng, H Zhang, G Zhang, LSM Huang, Y Xu, RT Schooley, J An, Z Huang
European Journal of Medicinal Chemistry, 2022-09-28;244(0):114797. 2022-09-28
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The serum irisin response to prolonged physical activity in temperate and hot environments in older men with hypertension or type 2 diabetes
Authors: JJ McCormick, KE King, SR Notley, N Fujii, P Boulay, RJ Sigal, T Amano, GP Kenny
Journal of thermal biology, 2022-09-24;110(0):103344. 2022-09-24
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Examination of the TIGIT-CD226-CD112-CD155 Immune Checkpoint Network during a Healthy Pregnancy
Authors: M Meggyes, DU Nagy, T Feik, A Boros, B Polgar, L Szereday
International Journal of Molecular Sciences, 2022-09-15;23(18):. 2022-09-15
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Paeoniflorin, a constituent of Kami-shoyo-san, suppresses blood glucose levels in postmenopausal diabetic mice by promoting the secretion of estradiol from adipocytes
Authors: K Kobayashi, YT Tang, K Sasaki
Oncogene, 2022-09-05;32(0):101335. 2022-09-05
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Periostin Augments Vascular Smooth Muscle Cell Calcification via beta-Catenin Signaling
Authors: I Alesutan, LA Henze, B Boehme, TTD Luong, D Zickler, B Pieske, KU Eckardt, A Pasch, J Voelkl
Biomolecules, 2022-08-21;12(8):. 2022-08-21
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SIRT1 suppresses in vitro decidualization of human endometrial stromal cells through the downregulation of forkhead box O1 expression
Authors: SL Yu, SI Lee, HW Park, SK Lee, TH Kim, J Kang, SR Park
Oncogene, 2022-07-12;22(3):100672. 2022-07-12
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The performance of soluble CD163 as a non-invasive biomarker of liver damage in chronically HCV and HCV/HIV infected subjects
Authors: V Cairoli, E De Matteo, P Casciato, B Ameigeiras, MV Preciado, P Valva
PLoS ONE, 2022-07-07;17(7):e0270911. 2022-07-07
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VEGF-C serum level is associated with response to bevacizumab maintenance therapy in primary ovarian cancer patients
Authors: Y Ding, L Oliveira-F, E Vettorazzi, K Legler, K Milde-Lang, L Woelber, A Jaeger, K Prieske, V Mueller, B Schmalfeld, S Kuerti
PLoS ONE, 2022-06-10;17(6):e0269680. 2022-06-10
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Association between vascular endothelial growth factor-mediated blood-brain barrier dysfunction and stress-induced depression
Authors: H Matsuno, S Tsuchimine, K O'Hashi, K Sakai, K Hattori, S Hidese, S Nakajima, S Chiba, A Yoshimura, N Fukuzato, M Kando, M Tatsumi, S Ogawa, N Ichinohe, H Kunugi, K Sohya
Molecular Psychiatry, 2022-05-26;0(0):. 2022-05-26
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Chitin-glucan supplementation improved postprandial metabolism and altered gut microbiota in subjects at cardiometabolic risk in a randomized trial
Authors: H Ranaivo, Z Zhang, M Alligier, L Van Den Be, M Sothier, S Lambert-Po, N Feugier, C Cuerq, C Machon, AM Neyrinck, B Seethaler, J Rodriguez, M Roumain, GG Muccioli, V Maquet, M Laville, SC Bischoff, J Walter, NM Delzenne, JA Nazare
Scientific Reports, 2022-05-25;12(1):8830. 2022-05-25
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Stratification of radiosensitive brain metastases based on an actionable S100A9/RAGE resistance mechanism
Authors: C Monteiro, L Miarka, M Perea-Garc, N Priego, P García-Góm, L Álvaro-Esp, A de Pablos-, N Yebra, D Retana, P Baena, C Fustero-To, O Graña-Cast, K Troulé, E Caleiras, P Tezanos, P Muela, E Cintado, JL Trejo, JM Sepúlveda, P González-L, L Jiménez-Ro, LM Moreno, O Esteban, Á Pérez-Núñe, A Hernández-, J Mazarico G, I Ferrer, R Suárez, EM Garrido-Ma, L Paz-Ares, C Dalmasso, E Cohen-Jona, A Siegfried, A Hegarty, S Keelan, D Varešlija, LS Young, M Mohme, Y Goy, H Wikman, J Fernández-, G Blasco, L Alcázar, C Cabañuz, SI Grivenniko, A Ianus, N Shemesh, CC Faria, R Lee, P Lorigan, E Le Rhun, M Weller, R Soffietti, L Bertero, U Ricardi, J Bosch-Barr, E Sais, E Teixidor, A Hernández-, A Calvo, J Aristu, SM Martin, A Gonzalez, O Adler, N Erez, RENACER, M Valiente
Nature Medicine, 2022-04-11;28(4):752-765. 2022-04-11
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The role of neutrophil elastase in aortic valve calcification
Authors: Y Liu, P Jiang, L An, M Zhu, J Li, Y Wang, Q Huang, Y Xiang, X Li, Q Shi, Y Weng
Journal of Translational Medicine, 2022-04-09;20(1):167. 2022-04-09
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Asthmatic bronchial smooth muscle increases rhinovirus replication within the bronchial epithelium
Authors: P Esteves, B Allard, A Celle, I Dupin, E Maurat, O Ousova, M Thumerel, JW Dupuy, T Leste-Lass, R Marthan, PO Girodet, T Trian, P Berger
Cell Reports, 2022-03-29;38(13):110571. 2022-03-29
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Hyaluronidase-1-mediated glycocalyx impairment underlies endothelial abnormalities in polypoidal choroidal vasculopathy
Authors: KX Wu, NJY Yeo, CY Ng, FWJ Chioh, Q Fan, X Tian, B Yang, G Narayanan, HM Tay, HW Hou, NR Dunn, X Su, CMG Cheung, C Cheung
Bmc Biology, 2022-02-15;20(1):47. 2022-02-15
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Exercise in the heat induces similar elevations in serum irisin in young and older men despite lower resting irisin concentrations in older adults
Authors: JJ McCormick, KE King, SR Notley, N Fujii, P Boulay, RJ Sigal, GP Kenny
Journal of thermal biology, 2022-01-15;104(0):103189. 2022-01-15
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DENV-Mimetic Polymersome Nanoparticles Bearing Multi-Epitope Lipopeptides Antigen as the Next-Generation Dengue Vaccine
Authors: NA Abdul Rahm, AS Mohamad No, ML Nordin, MCI Mohd Amin, AA Ahmad Fuaa, NA Muhammad A, N Marasini, F Azmi
Pharmaceutics, 2022-01-10;14(1):. 2022-01-10
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Increased Plasma Soluble PD-1 Concentration Correlates with Disease Progression in Patients with Cancer Treated with Anti-PD-1 Antibodies
Authors: R Ohkuma, K Ieguchi, M Watanabe, D Takayanagi, T Goshima, R Onoue, K Hamada, Y Kubota, A Horiike, T Ishiguro, Y Hirasawa, H Ariizumi, J Tsurutani, K Yoshimura, M Tsuji, Y Kiuchi, S Kobayashi, T Tsunoda, S Wada
Biomedicines, 2021-12-16;9(12):. 2021-12-16
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Streptozotocin-induced hyperglycemia alters the cecal metabolome and exacerbates antibiotic-induced dysbiosis
Authors: JI Wurster, RL Peterson, CE Brown, S Penumutchu, DV Guzior, K Neugebauer, WH Sano, MM Sebastian, RA Quinn, P Belenky
Cell Reports, 2021-12-14;37(11):110113. 2021-12-14
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Nordic Walking Rather Than High Intensity Interval Training Reduced Myostatin Concentration More Effectively in Elderly Subjects and the Range of This Drop Was Modified by Metabolites of Vitamin D
Authors: K Micielska, M Flis, JA Kortas, E Rodziewicz, J Antosiewic, K Wochna, G Lombardi, E Ziemann
Nutrients, 2021-12-08;13(12):. 2021-12-08
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Clinical and biological markers for predicting ARDS and outcome in septic patients
Authors: J Villar, R Herrán-Mon, E González-H, M Prieto-Gon, A Ambrós, A Rodríguez-, A Muriel-Bom, R Solano, C Cuenca-Rub, A Vidal, C Flores, JM González-M, MI García-Lao, Genetics o
Scientific Reports, 2021-11-22;11(1):22702. 2021-11-22
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Zinc Ameliorates the Osteogenic Effects of High Glucose in Vascular Smooth Muscle Cells
Authors: LA Henze, M Estepa, B Pieske, F Lang, KU Eckardt, I Alesutan, J Voelkl
Cells, 2021-11-09;10(11):. 2021-11-09
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NR4A family members regulate T cell tolerance to preserve immune homeostasis and suppress autoimmunity
Authors: R Hiwa, HV Nielsen, JL Mueller, R Mandla, J Zikherman
JCI Insight, 2021-09-08;0(0):. 2021-09-08
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Latent TGFbeta-binding proteins regulate UCP1 expression and function via TGFbeta2
Authors: D Halbgebaue, J Roos, JB Funcke, H Neubauer, BS Hamilton, E Simon, EZ Amri, KM Debatin, M Wabitsch, P Fischer-Po, D Tews
Molecular Metabolism, 2021-09-01;53(0):101336. 2021-09-01
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Structural Deformation of MTX Induced by Nanodrug Conjugation Dictate Intracellular Drug Transport and Drug Efficacy
Authors: JY Park, JS Hyun, JG Jee, SJ Park, D Khang
International Journal of Nanomedicine, 2021-07-22;16(0):4943-4957. 2021-07-22
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Bioengineered Nanoparticles Loaded-Hydrogels to Target TNF Alpha in Inflammatory Diseases
Authors: IM Oliveira, DC Fernandes, FR Maia, RF Canadas, RL Reis, JM Oliveira
Pharmaceutics, 2021-07-21;13(8):. 2021-07-21
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Interleukin-13 rs1800925/-1112C/T promoter single nucleotide polymorphism variant linked to anti-schistosomiasis in adult males in Murehwa District, Zimbabwe
Authors: ET Choto, T Mduluza, MJ Chimbari
PLoS ONE, 2021-05-28;16(5):e0252220. 2021-05-28
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Protection against SARS-CoV-2 infection by a mucosal vaccine in rhesus macaques
Authors: Y Sui, J Li, R Zhang, SK Prabhu, HA Elyard, D Venzon, A Cook, RE Brown, E Teow, J Velasco, J Greenhouse, T Putmon-Tay, TV Campbell, L Pessaint, IN Moore, LA Lagenaur, JD Talton, MW Breed, J Kramer, KW Bock, M Minai, BM Nagata, MG Lewis, LX Wang, JA Berzofsky
JCI Insight, 2021-04-28;0(0):. 2021-04-28
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Serial transplantation unmasks galectin-9 contribution to tumor immune escape in the MB49 murine model
Authors: V Baloche, J Rivière, TBT Tran, A Gelin, O Bawa, N Signolle, MBK Diop, P Dessen, S Beq, M David, P Busson
Scientific Reports, 2021-03-04;11(1):5227. 2021-03-04
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Macrophage migration inhibitory factor may play a protective role in osteoarthritis
Authors: M Liu, Z Xie, G Sun, L Chen, D Qi, H Zhang, J Xiong, A Furey, P Rahman, G Lei, G Zhai
Arthritis Research & Therapy, 2021-02-20;23(1):59. 2021-02-20
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Analysis of the behavior of 2D monolayers and 3D spheroid human pancreatic beta cells derived from induced pluripotent stem cells in a microfluidic environment
Authors: A Essaouiba, R Jellali, M Shinohara, B Scheidecke, C Legallais, Y Sakai, E Leclerc
Journal of biotechnology, 2021-02-19;0(0):. 2021-02-19
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Palladin isoforms 3 and 4 regulate cancer-associated fibroblast pro-tumor functions in pancreatic ductal adenocarcinoma
Authors: JI Alexander, DB Vendramini, R Francescon, T Luong, J Franco-Bar, N Shah, JC Gardiner, E Nicolas, KS Raghavan, E Cukierman
Scientific Reports, 2021-02-15;11(1):3802. 2021-02-15
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Protein Digests and Pure Peptides from Chia Seed Prevented Adipogenesis and Inflammation by Inhibiting PPAR&gamma and NF-&kappaB Pathways in 3T3L-1 Adipocytes
Authors: M Grancieri, HSD Martino, E Gonzalez d
Nutrients, 2021-01-08;13(1):. 2021-01-08
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Association of PARP1-specific polymorphisms and haplotypes with non-small cell lung cancer subtypes
Authors: J Jin, H Robeson, P Fagan, MS Orloff
PLoS ONE, 2020-12-07;15(12):e0243509. 2020-12-07
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New insights in gene expression alteration as effect of doxorubicin drug resistance in triple negative breast cancer cells
Authors: CA Ciocan-Car, A Jurj, O Zanoaga, R Cojocneanu, LA Pop, A Moldovan, C Moldovan, AA Zimta, L Raduly, C Pop-Bica, M Buse, L Budisan, P Virag, A Irimie, SMG Diaz, I Berindan-N, C Braicu
J Exp Clin Cancer Res, 2020-11-13;39(1):241. 2020-11-13
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Differentially expressed circular RNAs in a murine asthma model
Authors: H Bao, Q Zhou, Q Li, M Niu, S Chen, P Yang, Z Liu, L Xia
Molecular Medicine Reports, 2020-10-20;22(6):5412-5422. 2020-10-20
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Anti-Inflammatory Properties of Injectable Betamethasone-Loaded Tyramine-Modified Gellan Gum/Silk Fibroin Hydrogels
Authors: IM Oliveira, C Gonçalves, ME Shin, S Lee, RL Reis, G Khang, JM Oliveira
Biomolecules, 2020-10-17;10(10):. 2020-10-17
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Network analysis of the progranulin-deficient mouse brain proteome reveals pathogenic mechanisms shared in human frontotemporal dementia caused by GRN mutations
Authors: M Huang, E Modeste, E Dammer, P Merino, G Taylor, DM Duong, Q Deng, CJ Holler, M Gearing, D Dickson, NT Seyfried, T Kukar
Acta Neuropathol Commun, 2020-10-07;8(1):163. 2020-10-07
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Selective Increment of Synovial Soluble TYRO3 Correlates with Disease Severity and Joint Inflammation in Patients with Rheumatoid Arthritis
Authors: J Vullings, JP Vago, CEJ Waterborg, RM Thurlings, MI Koenders, PLEM van Lent, PM van der Kr, FA Amaral, FAJ van de Loo
J Immunol Res, 2020-09-11;2020(0):9690832. 2020-09-11
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NR4A nuclear receptors restrain B cell responses to antigen when second signals are absent or limiting
Authors: C Tan, R Hiwa, JL Mueller, V Vykunta, K Hibiya, M Noviski, J Huizar, JF Brooks, J Garcia, C Heyn, Z Li, A Marson, J Zikherman
Nat. Immunol., 2020-08-31;0(0):. 2020-08-31
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Modulation of Human Adipose Stem Cells' Neurotrophic Capacity Using a Variety of Growth Factors for Neural Tissue Engineering Applications: Axonal Growth, Transcriptional, and Phosphoproteomic Analyses In Vitro
Authors: KM Prautsch, A Schmidt, V Paradiso, DJ Schaefer, R Guzman, DF Kalbermatt, S Madduri
Cells, 2020-08-21;9(9):. 2020-08-21
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Contactin-1 Is Reduced in Cerebrospinal Fluid of Parkinson's Disease Patients and Is Present within Lewy Bodies
Authors: M Chatterjee, I van Steeno, E Huisman, L Oosterveld, H Berendse, WM van der Fl, M Del Campo, AW Lemstra, WDJ van de Ber, CE Teunissen
Biomolecules, 2020-08-12;10(8):. 2020-08-12
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Phase imaging of transition from classical to quantum plasmonic couplings between a metal nanoparticle and a metal surface
Authors: H Wang, H Yu, Y Wang, X Shan, HY Chen, N Tao
Proc. Natl. Acad. Sci. U.S.A., 2020-07-14;0(0):. 2020-07-14
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Increase of Antitumoral Effects of Cytokine-Induced Killer Cells by Antibody-Mediated Inhibition of MICA Shedding
Authors: X Wu, Y Zhang, Y Li, IGH Schmidt-Wo
Cancers (Basel), 2020-07-07;12(7):. 2020-07-07
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Topical Administration of SLN-Based Gene Therapy for the Treatment of Corneal Inflammation by De Novo IL-10 Production
Authors: M Vicente-Pa, I Gómez-Agua, J Rodríguez-, A Rodríguez-, E Muntoni, L Battaglia, A Del Pozo-R, MÁ Solinís As
Pharmaceutics, 2020-06-23;12(6):. 2020-06-23
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Induction of ASC pyroptosis requires gasdermin D or caspase-1/11-dependent mediators and IFNbeta from pyroptotic macrophages
Authors: C Zhang, C Zhao, X Chen, R Tao, S Wang, G Meng, X Liu, C Shao, X Su
Cell Death Dis, 2020-06-18;11(6):470. 2020-06-18
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Urinary proteomics links keratan sulfate degradation and lysosomal enzymes to early type 1 diabetes
Authors: JAD Van, S Clotet-Fre, AC Hauschild, I Batruch, I Jurisica, Y Elia, FH Mahmud, E Sochett, EP Diamandis, JW Scholey, A Konvalinka
PLoS ONE, 2020-05-26;15(5):e0233639. 2020-05-26
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Increased ratio of mature BDNF to precursor-BDNF in patients with major depressive disorder with severe anhedonia
Authors: C Wu, J Lu, S Lu, M Huang, Y Xu
J Psychiatr Res, 2020-05-12;126(0):92-97. 2020-05-12
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Blocking interaction between SHP2 and PD-1 denotes a novel opportunity for developing PD-1 inhibitors
Authors: Z Fan, Y Tian, Z Chen, L Liu, Q Zhou, J He, J Coleman, C Dong, N Li, J Huang, C Xu, Z Zhang, S Gao, P Zhou, K Ding, L Chen
EMBO Mol Med, 2020-05-11;0(0):e11571. 2020-05-11
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Iron Status in Elderly Women Impacts Myostatin, Adiponectin and Osteocalcin Levels Induced by Nordic Walking Training
Authors: J Kortas, E Ziemann, D Juszczak, K Micielska, M Koz?owska, K Prusik, K Prusik, J Antosiewic
Nutrients, 2020-04-17;12(4):. 2020-04-17
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Characterization of Human NK Cell-Derived Exosomes: Role of DNAM1 Receptor In Exosome-Mediated Cytotoxicity Against Tumor
Authors: ALD Pace, N Tumino, F Besi, C Alicata, LA Conti, E Munari, E Maggi, P Vacca, L Moretta
Cancers (Basel), 2020-03-12;12(3):. 2020-03-12
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Resistance to autosomal dominant Alzheimer's disease in an APOE3 Christchurch homozygote: a case report
Authors: JF Arboleda-V, F Lopera, M O'Hare, S Delgado-Ti, C Marino, N Chmielewsk, KL Saez-Torre, D Amarnani, AP Schultz, RA Sperling, D Leyton-Cif, K Chen, A Baena, D Aguillon, S Rios-Romen, M Giraldo, E Guzmán-Vél, DJ Norton, E Pardilla-D, A Artola, JS Sanchez, J Acosta-Uri, M Lalli, KS Kosik, MJ Huentelman, H Zetterberg, K Blennow, RA Reiman, J Luo, Y Chen, P Thiyyagura, Y Su, GR Jun, M Naymik, X Gai, M Bootwalla, J Ji, L Shen, JB Miller, LA Kim, PN Tariot, KA Johnson, EM Reiman, YT Quiroz
Nat. Med., 2019-11-04;0(0):. 2019-11-04
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A Virus-Derived Immune Modulating Serpin Accelerates Wound Closure with Improved Collagen Remodeling
Authors: L Zhang, JR Yaron, AM Tafoya, SE Wallace, J Kilbourne, S Haydel, K Rege, G McFadden, AR Lucas
J Clin Med, 2019-10-04;8(10):. 2019-10-04
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Basilar Artery Tortuosity Is Associated With White Matter Hyperintensities by TIMP-1
Authors: DP Zhang, YF Peng, HL Zhang, JG Ma, M Zhao, S Yin, TT Wei
Front Neurosci, 2019-08-14;13(0):836. 2019-08-14
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Generation of Human PSC-Derived Kidney Organoids with Patterned Nephron Segments and a De Novo Vascular Network
Authors: JH Low, P Li, EGY Chew, B Zhou, K Suzuki, T Zhang, MM Lian, M Liu, E Aizawa, C Rodriguez, KSM Yong, Q Chen, JM Campistol, M Fang, CC Khor, JN Foo, JC Izpisua Be, Y Xia
Cell Stem Cell, 2019-07-11;0(0):. 2019-07-11
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Apolipoprotein Profiles in Very Preterm and Term-Born Preschool Children
Authors: A Posod, R Pechlaner, X Yin, SA Burnap, SJ Kiechl, J Willeit, JL Witztum, M Mayr, S Kiechl, U Kiechl-Koh
J Am Heart Assoc, 2019-04-16;8(8):e011199. 2019-04-16
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Metabolic effects of leptin receptor knockdown or reconstitution in adipose tissues
Authors: S Pereira, SM O'Dwyer, TD Webber, RK Baker, V So, CE Ellis, JS Yoon, M Mojibian, MM Glavas, S Karunakara, SM Clee, SD Covey, TJ Kieffer
Sci Rep, 2019-03-01;9(1):3307. 2019-03-01
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Potential relation between soluble growth differentiation factor-15 and testosterone deficiency in male patients with coronary artery disease
Authors: H Liu, Y Lyu, D Li, Y Cui, Y Huang, W Dai, Y Li
Cardiovasc Diabetol, 2019-02-28;18(1):21. 2019-02-28
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Intracerebral Delivery of Brain-Derived Neurotrophic Factor Using HyStem�-C Hydrogel Implants Improves Functional Recovery and Reduces Neuroinflammation in a Rat Model of Ischemic Stroke
Authors: K Ravina, DI Briggs, S Kislal, Z Warraich, T Nguyen, RK Lam, TI Zarembinsk, M Shamloo
Int J Mol Sci, 2018-11-28;19(12):. 2018-11-28
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CUDC-907 blocks multiple pro-survival signals and abrogates microenvironment protection in CLL
Authors: Y Chen, C Peubez, V Smith, S Xiong, G Kocsis-Fod, B Kennedy, S Wagner, C Balotis, S Jayne, MJS Dyer, S Macip
J. Cell. Mol. Med., 2018-10-24;0(0):. 2018-10-24
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Salt-Responsive Metabolite, ?-Hydroxybutyrate, Attenuates Hypertension
Authors: S Chakrabort, S Galla, X Cheng, JY Yeo, B Mell, V Singh, B Yeoh, P Saha, AV Mathew, M Vijay-Kuma, B Joe
Cell Rep, 2018-10-16;25(3):677-689.e4. 2018-10-16
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Factor XIIIA-expressing inflammatory monocytes promote lung squamous cancer through fibrin cross-linking
Authors: A Porrello, PL Leslie, EB Harrison, BK Gorentla, S Kattula, SK Ghosh, SH Azam, A Holtzhause, YL Chao, MC Hayward, TA Waugh, S Bae, V Godfrey, SH Randell, C Oderup, L Makowski, J Weiss, MD Wilkerson, DN Hayes, HS Earp, AS Baldwin, AS Wolberg, CV Pecot
Nat Commun, 2018-05-18;9(1):1988. 2018-05-18
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Evidence that Graves' Ophthalmopathy Immunoglobulins Do Not Directly Activate IGF-1 Receptors
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Thyroid, 2018-04-30;0(0):. 2018-04-30
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Protective Role of the MER Tyrosine Kinase via Efferocytosis in Rheumatoid Arthritis Models
Authors: CEJ Waterborg, S Beermann, MGA Broeren, MB Bennink, MI Koenders, PLEM van Lent, WB van den Be, PM van der Kr, FAJ van de Loo
Front Immunol, 2018-04-13;9(0):742. 2018-04-13
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Loss of the Immune Checkpoint CD85j/LILRB1 on Malignant Plasma Cells Contributes to Immune Escape in Multiple Myeloma.
Authors: E Lozano, Lozano E, T Díaz, Diaz T, MP Mena, Mena M, G Suñe, Sune G, X Calvo, M Calderón, Calderon M, L Pérez-Amil, Perez-Amill L, V Rodríguez, P Pérez-Galá, G Roué, MT Cibeira, Cibeira M, L Rosiñol, Rosinol L, I Isola, Isola I, LG Rodríguez-, Rodriguez-Lobato L, B Martin-Ant, J Bladé, Blade J, C Fernández
J Immunol, 2018-03-12;200(8):2581-2591. 2018-03-12
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Combined serum levels of multiple proteins in tPA-BDNF pathway may aid the diagnosis of five mental disorders
Authors: S Chen, H Jiang, Y Liu, Z Hou, Y Yue, Y Zhang, F Zhao, Z Xu, Y Li, X Mou, L Li, T Wang, J Zhao, C Han, Y Sui, M Wang, Z Yang, Y Lu, Y Zhu, J Li, X Shen, F Sun, Q Chen, H Chen, Y Yuan
Sci Rep, 2017-07-31;7(1):6871. 2017-07-31
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Surface Toll-Like Receptor 3 Expression in Metastatic Intestinal Epithelial Cells induces Inflammatory Cytokine Production and Promotes Invasiveness
Authors: M Bugge, B Bergstrom, OK Eide, H Solli, IF Kjønstad, J Stenvik, T Espevik, NJ Nilsen
J. Biol. Chem., 2017-07-17;0(0):. 2017-07-17
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Lipopolysaccharide-Binding Protein Downregulates Fractalkine through Activation of p38 MAPK and NF-?B
Authors: X Huang, Y Zeng, Y Jiang, Y Qin, W Luo, S Xiang, SR Sooranna, L Pinhu
Mediators Inflamm., 2017-05-29;2017(0):9734837. 2017-05-29
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Soluble plasma proteins ST2 and CD163 as early biomarkers of nephropathy in Swedish patients with diabetes, 15-34�years of age: a prospective cohort study
Authors: M Samuelsson, J Dereke, MK Svensson, M Landin-Ols, M Hillman, on the beh
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Urinary kallikrein 10 predicts the incurability of gastric cancer
Authors: T Shimura, M Ebi, T Yamada, T Yamada, T Katano, Y Nojiri, H Iwasaki, S Nomura, N Hayashi, Y Mori, H Kataoka, MA Moses, T Joh
Oncotarget, 2017-04-25;8(17):29247-29257. 2017-04-25
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The serum protein levels of the tPA-BDNF pathway are implicated in depression and antidepressant treatment
Authors: H Jiang, S Chen, C Li, N Lu, Y Yue, Y Yin, Y Zhang, X Zhi, D Zhang, Y Yuan
Transl Psychiatry, 2017-04-04;7(4):e1079. 2017-04-04
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Plasminogen Activator Inhibitor-1 Protects Mice Against Cardiac Fibrosis by Inhibiting Urokinase-type Plasminogen Activator-mediated Plasminogen Activation
Authors: KK Gupta, DL Donahue, MJ Sandoval-C, FJ Castellino, VA Ploplis
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Polarization of macrophages towards M2 phenotype is favored by reduction in iPLA2?
J Biol Chem, 2016-09-20;0(0):. 2016-09-20
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View all ELISA FAQsReviews for DuoSet ELISA Ancillary Reagent Kit 2
Average Rating: 4.9 (Based on 86 Reviews)
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Works very well if protocol is followed. Another high binding plates can also be used optionally
Budget friendly and produces excellent results
Used with Mouse CXCL10 ELISA Kit DY446
Very good kit. It’s reasonable to buy this kit instead of buying everything separately. The result is reproducible.
This kit is very helpful. The protocol is also easy and straight forward
Results are very reproducible and the kit was very helpful and easy to use. This saved a lot of time, with minimal troubleshooting.
The results are reproducible between different Lots of this kit.
Results are reproducible and the kit is easy to follow.
Used for measuring IL2 and IFN-gamma release in T cell cultures.
The DuoSet ELISA Ancillary Reagent Kit 2 was used with the Human TNF-alpha DuoSet ELISA kit (DY210), Human IL-6 DuoSet ELISA (DY206) and Human IL-12/IL-23 p40 DuoSet ELISA (DY124) to detect secreted cytokines in GMCSF/IL4 monocyte-derived human dendritic cells stimulated with LPS (100 ng/ml) for 24 hours.
I use this for several different ELISAs. The duoset has worked well for every ELISA I have run.
Works well!!!
The Ancillary Kit2 that we purchased for in house assay development of our target proteins worked well. We are regularly using this product which is giving us reproducible results.
DuoSet ELISA Ancillary Reagent Kit 2 was used in combination with the Human TNF-alpha DuoSet ELISA kit (DY210). Monocytes were stimulated with zymosan (25 μg/ml) for 24 hours. After 24 hours, TNFa was assessed in the supernatant.
Used for quantifying release of IL2 and IFN gamma
Detection of IFN-g produced by BCMA-specific CAR-T cells in response to BCMA-negative and BCMA-positive hematopoietic cell lines.
Performance and reproducibility of the kit are very good (see graph attached). Also, very good customer service and fast shipment.
On the downside, the protocol is tricky to undstand.The information to perform the test is spread on three documents (the protocol, the certificate of analysis and the list of materials), so that pooling this information gives you quite the extra work.
Contains all the reagents needed for the experiments and works well!Well done!
Good standard curve even for linear form
we used this kit for developing sandwich ELISA's
Contains all the reagents needed and works well, p.s. nice ladder
The perfect companion to DuoSet ELISA kits, everything you need to run five plates.
I used this reagent pack with the Guinea Pig IL1B ELISA from R and D systems and it worked exactly as expected. I even extended the standard by two points and had no problems with the output.
Used for measuring NGF in bovine seminal plasma.
The plates provided are excellent to use; Reagents provided are sufficient and should be well allocated accordingly with preferably proper estimations for each ELISA run. Image attached indicates a sample of the product in use for ELISA
DY008 is used with DY1918-05 to perform fibronectin ELISA for cell culture supernatants in ARPE-19 cells stimulated with or without StemXVivo for 1-5 days.
We have thus far used this over 20 kits of DY008 for a range of different DuoSet ELISA kits and they are performing very good. There is little variation between the kits and they are a great compliment to the DuoSet kits. Highly recommended when app,licable.
Every reagent provided.
This ancillary kit works well as intended and the descriptions on the spec sheet have improved. It is a reliable kit.
The only issue is the volumes given are not very convenient for the DuoSet ELISA kit (at least for DY537). The ELISA requires at least 25 mL of Wash buffer per plate to comfortably use an autowasher and priming the system. However, this kit provides its wash buffer in 21 mL vials and a total of 126 mL for 5 plates when the ELISA would use 125 mL for 5 plates - a very close call that has caused problems.
The box it is provided in is more ergonomic now than its previous versions as well.
This was the first purchased the accessory kit when using the DuoSet ELISA kits. It was perfect, worth every penny and made performing ELISAs that much simpler.
Works very well with all compatible DuoSet kits.