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Human B7-1/CD80 Antibody

Catalog #: MAB140
14 Citations 1 Review Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB140-SP
MAB140-100
MAB140-500
Detection of B7‑1/CD80 in Raji Human Cell Line by Flow Cytometry.
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Product Details
Citations (14)
FAQs
Supplemental Products
Reviews (1)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human B7-1/CD80 Antibody Summary

Species Reactivity
Human
Specificity
Detects human B7‑1/CD80 in ELISAs. In sandwich immunoassays, no cross-reactivity or interference with recombinant human (rh) B7-2, recombinant mouse (rm) B7-1, rmB7-2, rhB7-H1, rhB7-H2, rhB7-H3 or rmPD-L2 is observed.
Source
Monoclonal Mouse IgG1 Clone # 37711
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human B7‑1/CD80
Val35-Asn242 (predicted)
Accession # P33681
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
See below
Immunohistochemistry
5-25 µg/mL
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Dual RNAscope ISH-IHC
5-25 µg/mL
See below

Human B7-1/CD80 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
2-8 µg/mL 

Use in combination with:

Detection Reagent: Human B7‑1/CD80 Biotinylated Antibody (Catalog # BAM1402)

Standard: Recombinant Human B7-1/CD80 Fc Chimera His-tag Protein, CF (Catalog # 140-B1)

Neutralization
Measured by its ability to neutralize B7‑1/CD80 and PHA-induced IL‑2 secretion in the Jurkat human acute T cell leukemia cell line. The Neutralization Dose (ND50) is typically 0.2-1 µg/mL in the presence of 1 µg/mL Recombinant Human B7‑1/CD80 Fc Chimera and 10 µg/mL PHA.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of B7‑1/CD80 antibody in Raji Human Cell Line antibody by Flow Cytometry. View Larger

Detection of B7‑1/CD80 in Raji Human Cell Line by Flow Cytometry. Raji human Burkitt's lymphoma cell line was stained with Mouse Anti-Human B7-1/CD80 Monoclonal Antibody (Catalog # MAB140, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Fluorescein-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0103B). View our protocol for Staining Membrane-associated Proteins.

Neutralization IL‑2 secretion Induced by B7‑1/CD80 and Neutralization by Human B7‑1/CD80 Antibody. View Larger

IL‑2 secretion Induced by B7‑1/CD80 and Neutralization by Human B7‑1/CD80 Antibody. Recombinant Human B7-1/CD80 Fc Chimera (Catalog # 140-B1) co-stimulates IL-2 secretion in the Jurkat human acute T cell leukemia cell line in the presence of PHA in a dose-dependent manner (orange line), as measured by the Human IL-2 Quantikine ELISA Kit (Catalog # D2050). IL-2 secretion elicited by Recombinant Human B7-1/CD80 Fc Chimera (1 µg/mL) and PHA (10 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human B7-1/CD80 Monoclonal Antibody (Catalog # MAB140). The ND50 is typically 0.2-1 µg/mL.

Immunohistochemistry B7-1/CD80 antibody in Human Tonsil by Immunohistochemistry (IHC-P). View Larger

B7‑1/CD80 in Human Tonsil. B7-1/CD80 was detected in immersion fixed paraffin-embedded sections of human tonsil using Mouse Anti-Human B7-1/CD80 Monoclonal Antibody (Catalog # MAB140) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in lymphocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Dual RNAscope ISH-IHC Compatible B7-1/CD80 antibody in Human Tonsil Using Dual RNAscope<sup>®</sup>ISH and IHC. View Larger

B7-1/CD80 in Human Tonsil Using Dual RNAscope®ISH and IHC. B7-1/CD80 mRNA was detected in formalin-fixed paraffin-embedded tissue sections of human tonsil probed with ACD RNAScope®Probe (Catalog # 42471) and stained using ACD RNAscope®2.5 HD Duplex Detection Reagents (red, Catalog # 322500). Adjacent tissue section was processed for immunohistochemistry using R&D Systems Mouse Anti-Human B7-1/CD80 Monoclonal Antibody (Catalog# MAB140) at 5ug/mL with overnight incubation at 4 °C followed by incubation with the Anti-Mouse IgG VisUCyte HRP Polymer Antibody (R&D Systems, Catalog # VC001) and DAB chromogen (lower image, brown). Tissues were counterstained with hematoxylin (blue).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: B7-1/CD80

B7-1 and B7-2, together with their receptors CD28 and CTLA-4, constitute one of the dominant co-stimulatory pathways that regulate T- and B-cell responses. Although both CTLA-4 and CD28 can bind to the same ligands, CTLA-4 binds to B7-1 and B7-2 with a 20‑100 fold higher affinity than CD28 and is involved in the down‑regulation of the immune response. B7-1 is expressed on activated B cells, activated T cells, and macrophages. B7-2 is constitutively expressed on interdigitating dendritic cells, Langerhans cells, peripheral blood dendritic cells, memory B cells, and germinal center B cells. Additionally, B7-2 is expressed at low levels on monocytes and can be up‑regulated through Interferon gamma. B7-1 and B7-2 are both members of the Immunoglobulin superfamily. Human B7-1 is a 288 amino acid (aa) protein containing a 34 aa signal peptide, a 208 aa extracellular domain, a 21 aa transmembrane domain, and a 25 aa cytoplasmic domain. Human B7-1 and B7-2 share 26% aa sequenceidentity. Human and mouse B7-1 share 44% aa sequenceidentity. However, it has been observed that both human and mouse B7-1 and B7-2 can bind to either human or mouse CD28 and CTLA-4, suggesting that there are conserved amino acids which form the B7-1/B7-2/CD28/CTLA-4 critical binding sites.

References
  1. Azuma, M. et al. (1993) Nature 366:76.
  2. Freeman, G.J. et al. (1993) Science 262:909.
  3. Freeman, G. et al. (1991) J. Exp. Med. 174:625.
  4. Selvakumar, A. et al. (1993) Immunogenetics 38:292.
  5. Chen, C. et al. (1994) J. Immunol. 152:4929.
  6. Freeman, G.J. et al. (1993) J. Exp. Med. 178:2185.
Entrez Gene IDs
941 (Human); 12519 (Mouse); 25408 (Rat); 102140078 (Cynomolgus Monkey)
Alternate Names
Activation B7-1 antigen; B7; B71; B7-1; BB1; CD28LG1; CD28LGB7-1 antigen); CD80 antigen; CD80 molecule; CD80; costimulatory factor CD80; costimulatory molecule variant IgV-CD80; CTLA-4 counter-receptor B7.1; T-lymphocyte activation antigen CD80

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Citations for Human B7-1/CD80 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

14 Citations: Showing 1 - 10
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  1. Ex vivo-expanded human CD19+TIM-1+ regulatory B cells suppress immune responses in vivo and are dependent upon the TIM-1/STAT3 axis
    Authors: S Shankar, J Stolp, SC Juvet, J Beckett, PS Macklin, F Issa, J Hester, KJ Wood
    Nature Communications, 2022-06-03;13(1):3121.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  2. Inhibiting MNK kinases promotes macrophage immunosuppressive phenotype to limit CD8+ T cell anti-tumor immunity
    Authors: TN Pham, C Spaulding, MA Shields, AE Metropulos, DN Shah, MG Khalafalla, DR Principe, DJ Bentrem, HG Munshi
    JCI Insight, 2022-05-09;0(0):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  3. B7-1 and programmed cell death-ligand 1 in primary and lymph node metastasis lesions of non-small cell lung carcinoma
    Authors: T Yamada, Y Miki, M Suzuki, O Kondoh, R Saito-Koya, K Ono, Y Okada, H Sasano
    Cancer Medicine, 2021-12-14;0(0):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  4. Tumour heterogeneity and intercellular networks of nasopharyngeal carcinoma at single cell resolution
    Authors: Y Liu, S He, XL Wang, W Peng, QY Chen, DM Chi, JR Chen, BW Han, GW Lin, YQ Li, QY Wang, RJ Peng, PP Wei, X Guo, B Li, X Xia, HQ Mai, XD Hu, Z Zhang, YX Zeng, JX Bei
    Nature Communications, 2021-02-02;12(1):741.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  5. PD-L1 engagement on T cells promotes self-tolerance and suppression of neighboring macrophages and effector T cells in cancer
    Authors: B Diskin, S Adam, MF Cassini, G Sanchez, M Liria, B Aykut, C Buttar, E Li, B Sundberg, RD Salas, R Chen, J Wang, M Kim, MS Farooq, S Nguy, C Fedele, KH Tang, T Chen, W Wang, M Hundeyin, JAK Rossi, E Kurz, MIU Haq, J Karlen, E Kruger, Z Sekendiz, D Wu, SAA Shadaloey, G Baptiste, G Werba, S Selvaraj, C Loomis, KK Wong, J Leinwand, G Miller
    Nat. Immunol., 2020-03-09;21(4):442-454.
    Species: Human, Mouse
    Sample Types: Whole Cells
    Applications: T Cell Activation
  6. The utility of urinary CD80 as a diagnostic marker in patients with renal diseases
    Authors: S Minamikawa, K Nozu, S Maeta, T Yamamura, K Nakanishi, J Fujimura, T Horinouchi, C Nagano, N Sakakibara, H Nagase, H Shima, K Noda, T Ninchoji, H Kaito, K Iijima
    Sci Rep, 2018-11-23;8(1):17322.
    Species: Human
    Sample Types: Urine
    Applications: ELISA Development (Detection)
  7. Comparison of the biological characteristics of human mesenchymal stem cells derived from exfoliated deciduous teeth, bone marrow, gingival tissue, and umbilical cord
    Authors: J Li, SQ Xu, YM Zhao, S Yu, LH Ge, BH Xu
    Mol Med Rep, 2018-09-20;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  8. Endothelial extracellular vesicles modulate the macrophage phenotype: Potential implications in atherosclerosis
    Authors: S He, C Wu, J Xiao, D Li, Z Sun, M Li
    Scand. J. Immunol., 2018-04-01;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  9. Tubular B7-1 expression parallels proteinuria levels, but not clinical outcomes in adult minimal change disease patients
    Authors: SW Lee, SH Baek, JH Paik, S Kim, KY Na, DW Chae, HJ Chin
    Sci Rep, 2017-02-02;7(0):41859.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  10. Autoantibodies against CD80 in patients with COPD
    Authors: Xiongbiao Wang
    Clin Transl Immunology, 2016-10-07;5(10):e103.
    Species: Human
    Sample Types: Serum
    Applications: Western Blot
  11. Tumor-activated TCRgammadelta(+) T cells from gastric cancer patients induce the antitumor immune response of TCRalphabeta(+) T cells via their antigen-presenting cell-like effects.
    Authors: Mao C, Mou X, Zhou Y, Yuan G, Xu C, Liu H, Zheng T, Tong J, Wang S, Chen D
    J Immunol Res, 2014-03-31;2014(0):593562.
    Species: Human
    Sample Types: Whole Cells
    Applications: Functional Assay
  12. B7-CD28 costimulatory signals control the survival and proliferation of murine and human gammadelta T cells via IL-2 production.
    J. Immunol., 2012-06-25;189(3):1202-8.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  13. ICOS-LICOS interaction is critically involved in TGN1412-mediated T-cell activation.
    Blood, 2012-05-10;119(26):6268-77.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  14. A synergistic antitumor effect of interleukin-2 addition with CD80 immunogene therapy for peritoneal metastasis of gastric carcinoma.
    Authors: Kosaka K, Yashiro M, Sakate Y, Hirakawa K
    Dig. Dis. Sci., 2007-04-03;52(8):1946-53.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization

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Human B7-1/CD80 Antibody
By Anonymous on 04/11/2022
Application: ICC/IF Sample Tested: Melanoma tissue Species: Human
Immunocytochemistry/Immunofluorescence Human B7-1/CD80 Antibody MAB140