Home / B7-1/CD80 / Recombinant Human B7-1/CD80 Fc Chimera His-tag Protein, CF

Recombinant Human B7-1/CD80 Fc Chimera His-tag Protein, CF

Catalog #: 140-B1
11 Citations 1 Review Datasheet / COA / SDS
Newer Version Available: 10133-B1
NEW

Discontinued Product

140-B1 has been discontinued and is replaced by 10133-B1. 9050-B1.

R&D Systems Recombinant Proteins and Enzymes
1 Image
Product Details
Citations (11)
FAQs
Reviews (1)
Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human B7-1/CD80 Fc Chimera His-tag Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to induce IL-2 secretion by Jurkat human acute T cell leukemia cells. Freeman, G.J. et al. (1993) Science 262:909. The ED50 for this effect is 0.025‑0.15 µg/mL in the presence of PHA.
Source
Mouse myeloma cell line, NS0-derived human B7-1/CD80 protein
Human B7-1
(Val35-Asn242)
Accession # NP_005182		 IEGRMD Human IgG1
(Pro100-Lys330) 		6-His tag
N-terminus C-terminus
Accession #
NP_005182
N-terminal Sequence
Analysis
Val35
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
51.3 kDa (monomer)
SDS-PAGE
70-90 kDa, reducing conditions

Product Datasheets

You must select a language.

x

140-B1

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

140-B1

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: B7-1/CD80

B7-1/CD80 and B7-2/CD86, together with their receptors CD28 and CTLA-4, constitute one of the dominant co-stimulatory pathways that regulate T- and B-cell responses (1). Although both CTLA-4 and CD28 can bind to the same ligands, CTLA-4 binds to B7-1 and B7-2 with a 20-100 fold higher affinity than CD28 and is involved in the down-regulation of the immune response (2-6). Mature human B7-1 consists of a 208 aa extracellular domain (ECD) with two immunoglobulin-like domains, a 21 aa transmembrane domain, and a 25 aa cytoplasmic domain (7). Within the ECD, human B7-1 shares 50% aa sequence identity with mouse and rat
B7-1. Alternative splicing generates a 30 kDa soluble isoform that lacks the transmembrane segment and retains the ability to bind CD28 and CTLA-4 and an isoform that lacks the second Ig-like domain and the transmembrane segment (8). Both human and mouse B7-1 and B7-2 can bind to either human or mouse CD28 and
CTLA-4 (1). B7-1 is expressed on activated B cells, activated T cells, and macrophages. B7-2 is constitutively expressed on interdigitating dendritic cells, Langerhans cells, peripheral blood dendritic cells, memory B cells, and germinal center B cells (2).

References
  1. Ville, S. et al. (2015) Front. Immunol. 6:411.
  2. Azuma, M. et al. (1993) Nature 366:76.
  3. Freeman, G.J. et al. (1993) Science 262:909.
  4. Chen, C. et al. (1994) J. Immunol. 152:4929.
  5. Freeman, G.J. et al. (1993) J. Exp. Med. 178:2185.
  6. Lanier, L. et al. (1995) J. Immunol. 154:97.
  7. Freeman, G.J. et al. (1989) J. Immunol. 143:2714.
  8. Kakoulidou, M. et al. (2007) Scand. J. Immunol. 66:529.
Entrez Gene IDs
941 (Human); 12519 (Mouse); 25408 (Rat); 102140078 (Cynomolgus Monkey)
Alternate Names
Activation B7-1 antigen; B7; B71; B7-1; BB1; CD28LG1; CD28LGB7-1 antigen); CD80 antigen; CD80 molecule; CD80; costimulatory factor CD80; costimulatory molecule variant IgV-CD80; CTLA-4 counter-receptor B7.1; T-lymphocyte activation antigen CD80

Citations for Recombinant Human B7-1/CD80 Fc Chimera His-tag Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

11 Citations: Showing 1 - 10
Filter your results:

Filter by:

  1. Nonimmune cell-derived ICOS ligand functions as a renoprotective alphavbeta3 integrin-selective antagonist
    Authors: KH Koh, Y Cao, S Mangos, NJ Tardi, RR Dande, HW Lee, B Samelko, MM Altintas, VP Schmitz, H Lee, K Mukherjee, V Peev, DJ Cimbaluk, J Reiser, E Hahm
    J. Clin. Invest., 2019-03-18;129(4):1713-1726.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Surface Plasmon Resonance (SPR
  2. An Activating Janus Kinase-3 Mutation Is Associated with Cytotoxic T Lymphocyte Antigen-4-Dependent Immune Dysregulation Syndrome
    Authors: H Sic, M Speletas, V Cornacchio, M Seidl, M Beibel, B Linghu, F Yang, E Sevdali, AE Germenis, EJ Oakeley, E Vangreveli, AW Sailer, E Traggiai, H Gram, H Eibel
    Front Immunol, 2017-12-15;8(0):1824.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Idelalisib and caffeine reduce suppression of T cell responses mediated by activated chronic lymphocytic leukemia cells
    Authors: BD Hock, SA MacPherson, JL McKenzie
    PLoS ONE, 2017-03-03;12(3):e0172858.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  4. A CD80-Biased CTLA4-Ig Fusion Protein with Superior In Vivo Efficacy by Simultaneous Engineering of Affinity, Selectivity, Stability, and FcRn Binding
    Authors: Lutz Jermutus
    J. Immunol., 2016-11-23;0(0):.
    Applications: Bioassay
  5. Genomic landscape of cutaneous T cell lymphoma.
    Authors: Choi J, Goh G, Walradt T, Hong B, Bunick C, Chen K, Bjornson R, Maman Y, Wang T, Tordoff J, Carlson K, Overton J, Liu K, Lewis J, Devine L, Barbarotta L, Foss F, Subtil A, Vonderheid E, Edelson R, Schatz D, Boggon T, Girardi M, Lifton R
    Nat Genet, 2015-07-20;47(9):1011-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. Establishment of long-term tolerance to SRBC in dogs by recombinant canine CTLA4-Ig.
    Authors: Graves SS, Stone D, Loretz C, Peterson L, McCune JS, Mielcarek M, Storb R
    Transplantation, 2009-08-15;88(3):317-22.
    Species: Canine
    Sample Types: Serum
    Applications: ELISA (Capture)
  7. Endoglycan, a member of the CD34 family of sialomucins, is a ligand for the vascular selectins.
    Authors: Kerr SC, Fieger CB, Snapp KR, Rosen SD
    J. Immunol., 2008-07-15;181(2):1480-90.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  8. Sulfated polysaccharides identified as inducers of neuropilin-1 internalization and functional inhibition of VEGF165 and semaphorin3A.
    Authors: Narazaki M, Segarra M, Tosato G
    Blood, 2008-02-13;111(8):4126-36.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Control
  9. The soluble forms of CD28, CD86 and CTLA-4 constitute possible immunological markers in patients with abdominal aortic aneurysm.
    Authors: Sakthivel P, Shively V, Kakoulidou M
    J. Intern. Med., 2007-04-01;261(4):399-407.
    Applications: ELISA (Standard)
  10. ICOS cooperates with CD28, IL-2, and IFN-gamma and modulates activation of human naive CD4+ T cells.
    Authors: Mesturini R, Nicola S, Chiocchetti A, Bernardone IS, Castelli L, Bensi T, Ferretti M, Comi C, Dong C, Rojo JM, Yagi J, Dianzani U
    Eur. J. Immunol., 2006-10-01;36(10):2601-12.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  11. Ligand-induced internalization selects use of common receptor neuropilin-1 by VEGF165 and semaphorin3A.
    Authors: Narazaki M, Tosato G
    Blood, 2006-01-19;107(10):3892-901.
    Applications: ELISA (Standard)

FAQs

No product specific FAQs exist for this product, however you may

View all Proteins and Enzyme FAQs

Reviews for Recombinant Human B7-1/CD80 Fc Chimera His-tag Protein, CF

Average Rating: 5 (Based on 1 Review)

5 Star
100%
4 Star
0%
3 Star
0%
2 Star
0%
1 Star
0%

Have you used Recombinant Human B7-1/CD80 Fc Chimera His-tag Protein, CF?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Filter by:


Recombinant Human B7-1/CD80 Fc Chimera His-tag Protein, CF
By Sarah Garrett on 11/15/2019
Application: Binding assay/Protein-protein interaction

Cells expressing a negative control or hCTLA-4 as mCherry fusions were treated with 0.25 ug of hB7-1 hIgG1. Cells were analyzed by flow cytometry with binding detected using an anti-human 488 secondary antibody.

Binding assay/Protein-protein interaction Recombinant Human B7-1/CD80 Fc Chimera His-tag Protein, CF 140-B1