Human BDNF Biotinylated Antibody

Catalog # Availability Size / Price Qty
BAM648
Product Details
Citations (3)
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Human BDNF Biotinylated Antibody Summary

Species Reactivity
Human
Specificity
Detects human BDNF in ELISAs. In sandwich ELISAs, no cross-reactivity was observed with  recombinant human (rh) beta -NGF, rhNT-3, or rhNT-4.
Source
Monoclonal Mouse IgG2A Clone # 37141
Purification
Protein A or G purified from ascites
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human BDNF
Arg128-Arg247
Accession # P23560
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample

Human BDNF Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Detection (Matched Antibody Pair)
0.5-2.0 µg/mL 

Use in combination with:

Capture Reagent: Human BDNF Antibody (Catalog # MAB848R)

Standard: Recombinant Human BDNF Protein (Catalog # 248-BD)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BDNF

Brain-derived neurotrophic factor (BDNF) is a member of the NGF family of neurotrophic factors (also named neurotrophins) that are required for the differentiation and survival of specific neuronal subpopulations in both the central as well as the peripheral nervous system. The neurotrophin family is comprised of at least four proteins including NGF, BDNF, NT-3, and NT-4/5. These secreted cytokines are synthesized as prepropeptides that are proteolytically processed to generate the mature proteins. All neurotrophins have six conserved cysteine residues that are involved in the formation of three disulfide bonds and all share approximately 55% sequence identity at the amino acid level. Similarly to NGF, bioactive BDNF is predicted to be a non-covalently linked homodimer.

BDNF cDNA encodes a 247 amino acid residue precursor protein with a signal peptide and a proprotein that are cleaved to yield the 119 amino acid residue mature BDNF. The amino acid sequence of mature BDNF is identical in all mammals examined. High levels of expression of BDNF have been detected in the hippocampus, cerebellum, fetal eye and placenta. In addition, low levels of BDNF expression are also found in the pituitary gland, spinal cord, heart, lung and skeletal muscle. BDNF binds with high affinity and specifically activates the TrkB tyrosine kinase receptor.

References
  1. Eide, F.F. et al. (1993) Exp. Neurol. 121:200.
  2. Snider, W.D. (1994) Cell 77:627.
  3. Barbacid, M. (1994) J. Neurobiol. 25:1386.
Long Name
Brain-derived Neurotrophic Factor
Entrez Gene IDs
627 (Human); 12064 (Mouse); 24225 (Rat)
Alternate Names
Abrineurin; ANON2; BDNF; brain-derived neurotrophic factor; BULN2; MGC34632; Neurotrophin

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Citations for Human BDNF Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Cannabinoids prevent depressive-like symptoms and alterations in BDNF expression in a rat model of PTSD
    Authors: O Burstein, N Shoshan, R Doron, I Akirav
    Prog. Neuropsychopharmacol. Biol. Psychiatry, 2018-02-17;84(0):129-139.
    Species: Rat
    Sample Types: Tissue Homogenates
    Applications: ELISA Detection
  2. Illness severity and biomarkers in depression: Using a unidimensional rating scale to examine BDNF
    Authors: MA Caldieraro, EA Vares, LH Souza, L Spanemberg, TA Guerra, B Wollenhaup, P Ferrari, AA Nierenberg, MP Fleck
    Compr Psychiatry, 2017-03-06;75(0):46-52.
    Species: Human
    Sample Types: Serum
    Applications: ELISA Development (Detection)
  3. BDNF plasma levels in acute stroke.
    Authors: Di Lazzaro V, Profice P, Pilato F, Dileone M, Florio L, Tonali PA, Angelucci F
    Neurosci. Lett., 2007-06-08;422(2):128-30.
    Species: Human
    Sample Types: Plasma
    Applications: ELISA Development

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