Human Isocitrate Dehydrogenase 2/IDH2 Antibody

Catalog #: MAB11462 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB11462-100
MAB11462-SP

Detection of Isocitrate Dehydrogenase 2/IDH2 in Jurkat cells by Flow Cytometry

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All Isocitrate Dehydrogenase 2/IDH2 Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Isocitrate Dehydrogenase 2/IDH2 Antibody Summary

Species Reactivity

Human

Specificity

Detects human IDH-2 in direct ELISA.

Source

Monoclonal Mouse IgG_2B Clone # 1069032

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human IDH-2
Thr376-Gln452
Accession # P48735

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Immunohistochemistry

3-25 µg/mL

Immersion fixed paraffin-embedded sections of human kidney

Immunocytochemistry

3-25 µg/mL

Fixed MCF-7 human breast cancer cell line (Positive) and SK-Mel-28 human malignant melanoma cell line (Positive)

Intracellular Staining by Flow Cytometry

0.25 µg/10⁶ cells

Jurkat human acute T cell leukemia cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry

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Detection of Isocitrate Dehydrogenase 2/IDH2 in Jurkat cells by Flow Cytometry Jurkat cells were stained with Mouse Anti-Human Isocitrate Dehydrogenase 2/IDH2 Monoclonal Antibody (Catalog # MAB11462, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Immunocytochemistry/ Immunofluorescence

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Detection of Isocitrate Dehydrogenase 2/IDH2 in MCF-7 cells (Positive) and SK-MEL-28 cells (Positive). Isocitrate Dehydrogenase 2/IDH2 was detected in fixed MCF‑7 human breast cancer cell line (Positive) and SK‑Mel‑28 human malignant melanoma cell line (Positive) using Mouse Anti-Human Isocitrate Dehydrogenase 2/IDH2 Monoclonal Antibody (Catalog # MAB11462) at 8 µg/ml for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to the cytoplasm and membrane. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunohistochemistry

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Detection of Isocitrate Dehydrogenase 2/IDH2 in Human Kidney. Isocitrate Dehydrogenase 2/IDH2 was detected in immersion fixed paraffin-embedded sections of human kidney using Mouse Anti-Human Isocitrate Dehydrogenase 2/IDH2 Monoclonal Antibody (Catalog # MAB11462) at 5 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm and kidney tubules. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Isocitrate Dehydrogenase 2/IDH2

Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP(+)-dependent isocitrate dehydrogenase found in the mitochondria. It plays a role in intermediary metabolism and energy production. This protein may tightly associate or interact with the pyruvate dehydrogenase complex.

Long Name

Isocitrate Dehydrogenase 2

Entrez Gene IDs

3418 (Human)

Alternate Names

D2HGA2; ICD-M; IDH2; IDHM; IDPM; Isocitrate Dehydrogenase 2