Human Latent TGF-beta bp1 APC-conjugated Antibody

Catalog #: IC388A Datasheet / COA / SDS

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IC388A

Detection of Latent TGF‑ beta bp1 in PC‑3 Human Cell Line by Flow Cytometry.

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Human Latent TGF-beta bp1 APC-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human LTBP-1 from the large latent TGF-beta 1 complex.

Source

Monoclonal Mouse IgG₁ Clone # 35409

Purification

Protein A or G purified from ascites

Immunogen

Human platelet-derived large latent TGF-beta 1 complex

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration

Sample

Intracellular Staining by Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry

Detection of Latent TGF-beta bp1 antibody in PC-3 Human Cell Line antibody by Flow Cytometry.

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Detection of Latent TGF‑ beta bp1 in PC‑3 Human Cell Line by Flow Cytometry. PC-3 human prostate cancer cell line was stained with Mouse Anti-Human Latent TGF-beta bp1 APC-conjugated Monoclonal Antibody (Catalog # IC388A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). View our protocol for Staining Intracellular Molecules.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: Latent TGF-beta bp1

TGF-beta is synthesized as high molecular weight latent complexes. In platelets, the large latent TGF-beta 1 complex is composed of three components: mature TGF-beta 1 dimer; latency associated peptide; and a latent TGF-beta binding protein (LTBP) (1-3).The cDNAs for four binding proteins (LTBP-1, -2, -3 and -4) have now been cloned (4-5).

References

Miyazono, K. et al. (1991) EMBO J. 10:1091.
Moren, A. et al. (1994) J. Biol. Chem. 269:32469.
Yin, W. et al. (1995) J. Biol. Chem. 270:10147.
Kanzaki, T. et al. (1990) Cell 61:1051.
Saharinen, J. et al. (1998) J. Biol. Chem. 273:18459.

Entrez Gene IDs

4052 (Human)

Alternate Names

Latent TGFbeta bp1; Latent TGF-beta bp1; latent transforming growth factor beta binding protein 1; latent-transforming growth factor beta-binding protein 1; LTBP1; LTBP-1; MGC163161; TGF-beta1-BP-1; Transforming growth factor beta-1-binding protein 1