Human/Mouse Brachyury Alexa Fluor® 488-conjugated Antibody

Catalog # Availability Size / Price Qty
IC2085G
Detection of Brachyury in D3 Mouse Cell Line by Flow Cytometry.
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Product Details
Citations (4)
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Human/Mouse Brachyury Alexa Fluor® 488-conjugated Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human Brachyury in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross-reactivity with recombinant human (rh) TBX-6 is observed, and approximately 5% cross-reactivity with rhTBX-2, rhTBX-5, and rhTBX-18 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human Brachyury
Ser2-Glu202
Accession # O15178
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 488 (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration
Sample
Intracellular Staining by Flow Cytometry
5 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry Detection of Brachyury antibody in D3 Mouse Cell Line antibody by Flow Cytometry. View Larger

Detection of Brachyury in D3 Mouse Cell Line by Flow Cytometry. D3 mouse embryonic stem cell line differentiated with mouse serum for 4 days was stained with Goat Anti-Human/Mouse Brachyury Alexa Fluor® 488-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # IC2085G, filled histogram) or isotype control antibody (Catalog # IC108G, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Brachyury

Brachyury is the founding member of the T-box family of transcription factors, which is characterized by the N-terminal conserved DNA-binding T-domain. Brachyury is required in the early determination and differentiation of mesoderms. Human brachyury molecule shares 90% homology with mouse brachyury.

Entrez Gene IDs
6862 (Human)
Alternate Names
brachyury protein; Brachyury; MGC104817; Protein T; SAVA; T brachyury (mouse) homolog; T brachyury homolog; T Brachyury Transcription Factor; T, brachyury homolog (mouse); T-Box Transcription Factor T; TBXT; TF; TFT

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Product Specific Notices


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

Citations for Human/Mouse Brachyury Alexa Fluor® 488-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Bat pluripotent stem cells reveal unusual entanglement between host and viruses
    Authors: Marion Déjosez, Arturo Marin, Graham M. Hughes, Ariadna E. Morales, Carlos Godoy-Parejo, Jonathan L. Gray et al.
    Cell
  2. Connexin 43 Gene Ablation Does Not Alter Human Pluripotent Stem Cell Germ Lineage Specification
    Authors: Grace A. Christopher, Rebecca J. Noort, Jessica L. Esseltine
    Biomolecules
  3. Single-cell qPCR demonstrates that Repsox treatment changes cell fate from endoderm to neuroectoderm and disrupts epithelial-mesenchymal transition
    Authors: Q Li, Q Huang
    PLoS ONE, 2019-10-10;14(10):e0223724.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Generation of heterozygous and homozygous hESC H9 sublines carrying inactivating mutations in RB1
    Authors: L Schipper, D Kanber, L Steenpass
    Stem Cell Res, 2018-09-26;33(0):41-45.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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